We narrowed to 581 results for: SUB
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TypeBlog Post.... It is important to note that the person who submitted the idea did not have to be in charge of implementing...
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Antibodies 101: Immunoprecipitation
TypeBlog Post...antibody that will be used for detection in a subsequent Western blot. When choosing beads, there are ... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog Post... the General Meeting seven other times I had subsequently changed fields and my cohort of friends and ... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post...patients from whom the blood is obtained and the subsequent reprogramming success [1]. Fibroblasts need to... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post...surprising. Consortia of higher education institutions submitted letters to the Department of Labor (for example... -
Supporting Reproducibility with a Connected ELN
TypeBlog Post...significantly enhances the ability of others to subsequently reproduce the research. A third, powerful example... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog Post...decision determines whether the client invests substantial monetary and human resources towards the development... -
Tips for Using FRET in Your Experiments
TypeBlog Post... Constructs to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog Post...polymerases which are each responsible for a specific subset of RNAs. To gain this specificity, the eukaryotic... -
Hot Plasmids: Summer 2024
TypeBlog Post...nervous system is composed of myriad cell types and subtypes, and a familiar challenge for anyone who has done... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog Post... of Ccd42 (Bendezú et al., 2015) and the alpha subunit of heterotrimeric G-proteins (Janetopoulos et al... -
CRISPR 101: Homology Directed Repair
TypeBlog Post... ssDNA 3’ overhang. This will serve as both a substrate for proteins required for strand invasion and ... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog Post...used to purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific... -
Evolution of Lab Techniques
TypeBlog Post...screen multiple colonies before selecting a smaller subset to sequence. Sequencing gels and next-generation... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post...course of infection, when HIV-1 is limited to a subset of T-cells, but it is unclear if enough T-cells... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post...had been more cognizant of this during my PhD. Submitting a publication doesn’t have to be like throwing... -
Hot Plasmids: Spring 2025
TypeBlog Post... to target and manipulate distinct interneuron subtypes. Neuron, 113(10), 1525–1547.e15. doi: https://... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog Post...efficiently integrate into the DNA through an HDR subpathway. It's traditionally been difficult to produce... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post... J., & Trimmer, J. S. (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog Post...marker such as thymidine kinase, a researcher may subsequently kill off any cells that still contain the donor...