We narrowed to 581 results for: SUB

1. Viral Vectors 101: Pseudotyping

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   ...rabies dG enters this neuron, it cannot infect any subsequent neurons because it does not express rabies glycoprotein...

2. CRISPR-based Genome Editing Systems For Editing in E. coli

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   ...resulting in cutting of the plasmid. At this point, subsequent introductions of pTargetF containing another ...

3. Supporting Reproducibility with a Connected ELN

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   ...significantly enhances the ability of others to subsequently reproduce the research. A third, powerful example...

4. Fluorescent Proteins 101: Aptamer Fluorophores

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   ...quencher from the proximity of the fluorophore and subsequently leading to a positive signal. The same effect...

5. Changing Labor Laws Bring Increased Postdoc Wages

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   ...surprising. Consortia of higher education institutions submitted letters to the Department of Labor (for example...

6. Healthcare Consulting: A Door to the Business of Life Sciences

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   ...decision determines whether the client invests substantial monetary and human resources towards the development...

7. Tips for Using FRET in Your Experiments

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   ... Constructs to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian...

8. Plasmids 101: The Promoter Region – Let's Go!

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   ...polymerases which are each responsible for a specific subset of RNAs. To gain this specificity, the eukaryotic...

9. Hot Plasmids: Summer 2024

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   ...nervous system is composed of myriad cell types and subtypes, and a familiar challenge for anyone who has done...

10. CRISPR 101: Homology Directed Repair

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    ... ssDNA 3’ overhang. This will serve as both a substrate for proteins required for strand invasion and ...

11. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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    ... of Ccd42 (Bendezú et al., 2015) and the alpha subunit of heterotrimeric G-proteins (Janetopoulos et al...

12. Multiple Plasmids at a Low Price: Kits from Addgene

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    ...used to purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific...

13. Evolution of Lab Techniques

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    ...screen multiple colonies before selecting a smaller subset to sequence. Sequencing gels and next-generation...

14. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    ...course of infection, when HIV-1 is limited to a subset of T-cells, but it is unclear if enough T-cells...

15. Thoughts on the Future of Research 2015 Boston Symposium

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    ...had been more cognizant of this during my PhD. Submitting a publication doesn’t have to be like throwing...

16. Hot Plasmids: Spring 2025

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    ... to target and manipulate distinct interneuron subtypes. Neuron, 113(10), 1525–1547.e15. doi: https://...

17. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    ...designs and administration doses. Don't forget to submit your validation data after your experiment! Thanks...

18. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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    ...efficiently integrate into the DNA through an HDR subpathway. It's traditionally been difficult to produce...

19. Antibodies 101: Multiplex Immunofluorescence

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    ... J., & Trimmer, J. S. (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

20. PiggyBac-ing Through the Genome Editing Field

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    ...marker such as thymidine kinase, a researcher may subsequently kill off any cells that still contain the donor...