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Showing: 541 - 560 of 577 results

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Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

Type

Blog Post

... Toxicity is likely due to DNA damage and its subsequent repair. Additionally, the distribution of enrichment...
Viral Vectors 101: Pseudotyping

Type

Blog Post

...rabies dG enters this neuron, it cannot infect any subsequent neurons because it does not express rabies glycoprotein...
CRISPR-based Genome Editing Systems For Editing in E. coli

Type

Blog Post

...resulting in cutting of the plasmid. At this point, subsequent introductions of pTargetF containing another ...
Rett Syndrome: A History of Research and Therapeutic Outlooks

Type

Blog Post

...the mutant MECP2 RNA in each of three neuronal subtypes of the hippocampus. This technology has formed...
Transferable Skills Guide: Creativity

Type

Blog Post

.... It is important to note that the person who submitted the idea did not have to be in charge of implementing...
Antibodies 101: Immunoprecipitation

Type

Blog Post

...antibody that will be used for detection in a subsequent Western blot. When choosing beads, there are ...
Overcoming the AAV Size Limitation for CRISPR Delivery

Type

Blog Post

...genome modification, but they did so without a substantial immune response or toxicity. More AAV-based CRISPR...
Tips for Using FRET in Your Experiments

Type

Blog Post

... Constructs to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian...
Plasmids 101: The Promoter Region – Let's Go!

Type

Blog Post

...polymerases which are each responsible for a specific subset of RNAs. To gain this specificity, the eukaryotic...
Multiple Plasmids at a Low Price: Kits from Addgene

Type

Blog Post

...used to purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific...
Hot Plasmids: Summer 2024

Type

Blog Post

...nervous system is composed of myriad cell types and subtypes, and a familiar challenge for anyone who has done...
CRISPR 101: Homology Directed Repair

Type

Blog Post

... ssDNA 3’ overhang. This will serve as both a substrate for proteins required for strand invasion and ...
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Type

Blog Post

... of Ccd42 (Bendezú et al., 2015) and the alpha subunit of heterotrimeric G-proteins (Janetopoulos et al...
Evolution of Lab Techniques

Type

Blog Post

...screen multiple colonies before selecting a smaller subset to sequence. Sequencing gels and next-generation...
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Type

Blog Post

...course of infection, when HIV-1 is limited to a subset of T-cells, but it is unclear if enough T-cells...
Thoughts on the Future of Research 2015 Boston Symposium

Type

Blog Post

...had been more cognizant of this during my PhD. Submitting a publication doesn’t have to be like throwing...
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

Type

Blog Post

...efficiently integrate into the DNA through an HDR subpathway. It's traditionally been difficult to produce...
Antibodies 101: Multiplex Immunofluorescence

Type

Blog Post

... J., & Trimmer, J. S. (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...
PiggyBac-ing Through the Genome Editing Field

Type

Blog Post

...marker such as thymidine kinase, a researcher may subsequently kill off any cells that still contain the donor...
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

Type

Blog Post

...non-homologous sequence or a series of single-nucleotide substitutions along a 1.5 kb tract, all without selectable...

Showing: 541 - 560 of 577 results

Results per page:

Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

Viral Vectors 101: Pseudotyping

CRISPR-based Genome Editing Systems For Editing in E. coli

Rett Syndrome: A History of Research and Therapeutic Outlooks

Transferable Skills Guide: Creativity

Antibodies 101: Immunoprecipitation

Overcoming the AAV Size Limitation for CRISPR Delivery

Tips for Using FRET in Your Experiments

Plasmids 101: The Promoter Region – Let's Go!

Multiple Plasmids at a Low Price: Kits from Addgene

Hot Plasmids: Summer 2024

CRISPR 101: Homology Directed Repair

Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Evolution of Lab Techniques

Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Thoughts on the Future of Research 2015 Boston Symposium

Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

Antibodies 101: Multiplex Immunofluorescence

PiggyBac-ing Through the Genome Editing Field

An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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