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Addgene
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  1. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ... cells (for example, plasmid with a puromycin resistance cassette, such as PX459). After you co-transfect...
  2. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...like... Bacterial Transformation Agarose Gel Electrophoresis Agarose Gel DNA Purification Streaking and ...purity click here . Top Protocol: Kit-free Alkaline Lysis Plasmid Miniprep Prepare the following solutions...phase Middle phase - A white layer may appear, consisting of precipitated protein particles Bottom phase...
  3. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...non-integrating. Read on to find out more! Molecular basis of transduction Transductions utilize the ‘normal...
  4. What the HEK?

    Type
    Blog Post
    ...many immature neuron-like qualities, which is inconsistent with kidney cells (Shaw et al.). Since then,...
Showing: 541 - 560 of 729 results