We narrowed to 738 results for: Sis;
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TypeBlog Post... away from PI driven basic research, there is resistance to change, public support is weak, government...
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SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog Post...nature, with the ultimate goal to improve the diagnosis of disease, and cancer in particular. They recently... -
9 Tips to Achieve Success in Academia
TypeBlog Post...aware of careers outside of academia. How do PIs assist their mentees who have goals outside of the university... -
Virus Protocol - Generating Stable Cell Lines
TypeProtocol...lentiviral vectors deliver mammalian antibiotic resistance (e.g., puromycin, blasticidin), which enables...not all lentiviral vectors deliver antibiotic resistance. This protocol was established using 293T cells...transgene expression. As polyclonal populations of resistant cells start proliferating and the individual wells... -
Understanding GPCR Signaling Using TRUPATH
TypeBlog Post...(Olsen RHJ et al., 2020). A transducer complex consists of a trimer of three subunits: alpha, beta, and... -
What's the Best Way to Elute and Store Your Plasmid DNA?
TypeBlog Post...mechanism called depurination. In depurination, the hydrolysis of the glycosidic bond leads to the release of... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post...2011) Power tools for gene expression and clonal analysis in Drosophila. Nature Methods 9:47–55 . https:... -
The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place
TypeBlog Post...for the user. This approach serves to maintain consistency with published screen interpretations and allows... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog Post..., fast, and easy. In cases where the mutational basis of a disease is known – repeat expansion, gene knock... -
Cellosaurus: A Cell Line Information Database
TypeBlog Post...ever been the target of an ‘-omics’ study or is resistant to specific compounds, links to publication references... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog Post...investigating genetic mechanisms related to morphogenesis, cell fate specification, and human disease.... -
CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
TypeBlog Post...configuration. In addition, D10A editing efficiency is consistently higher than that of H840A, especially with smaller... -
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog Post...through our Snapgene powered maps and sequence analysis tools. The main ways we use SnapGene for... -
Oh, The Places You Can Go: Careers in Science Communication - Product Development
TypeBlog Post...from home and looking for a job.” But Caitlin’s persistence paid off: “I spent about 6 months just editing... -
Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog Post...performance data, protocols, and even certificates of analysis.” Han’s biggest piece of advice for those looking... -
Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog Post... provides unmatched customer service that is consistently timely, informative, friendly, and professional... -
Bricking Science: Portraying Scientific Reality Through LEGO
TypeBlog Post...is the most approachable for LEGOs. Stop motion consists of taking many pictures, one for each position... -
ReFigure: Save Scientific Figures into Dashboards and Share Your Insights
TypeBlog Post...Osuna collated Zika case studies, making a meta analysis available to the community on ReFigure. ReFigure... -
Hot Plasmids - October 2022
TypeBlog Post...high-throughput screening platform to apply saturating mutagenesis on 21 positions in the protein sequence of the... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog Post...put on typical middle age weight and “exercise” consisted of taking walks in the mall. I took action, joined...