We narrowed to 731 results for: Sis;

1. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

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   Blog Post

   ...through our Snapgene powered maps and sequence analysis tools.     The main ways we use SnapGene for...

2. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

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   Blog Post

   ... provides unmatched customer service that is consistently timely, informative, friendly, and professional...

3. CRISPR 101: Drug discovery and CRISPR

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   Blog Post

   ..., fast, and easy. In cases where the mutational basis of a disease is known – repeat expansion, gene knock...

4. Cellosaurus: A Cell Line Information Database

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   Blog Post

   ...ever been the target of an ‘-omics’ study or is resistant to specific compounds, links to publication references...

5. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

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   Blog Post

   ...Osuna collated Zika case studies, making a meta analysis available to the community on ReFigure. ReFigure...

6. Oh, The Places You Can Go: Careers in Science Communication - Product Development

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   Blog Post

   ...from home and looking for a job.” But Caitlin’s persistence paid off: “I spent about 6 months just editing...

7. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

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   Blog Post

   ...performance data, protocols, and even certificates of analysis.” Han’s biggest piece of advice for those looking...

8. Bricking Science: Portraying Scientific Reality Through LEGO

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   Blog Post

   ...is the most approachable for LEGOs. Stop motion consists of taking many pictures, one for each position...

9. Hot Plasmids - October 2022

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   Blog Post

   ...high-throughput screening platform to apply saturating mutagenesis on 21 positions in the protein sequence of the...

10. Understanding GPCR Signaling Using TRUPATH

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    Blog Post

    ...(Olsen RHJ et al., 2020). A transducer complex consists of a trimer of three subunits: alpha, beta, and...

11. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

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    Blog Post

    ...for the user. This approach serves to maintain consistency with published screen interpretations and allows...

12. Running for Rare Disease, Running for FOP, Running for AJ

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    Blog Post

    ...put on typical middle age weight and “exercise” consisted of taking walks in the mall. I took action, joined...

13. Management for Scientists: Giving Feedback

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    Blog Post

    ...Feedback is help and it should result in authentic assistance for the recipient to become more effective and...

14. Choosing Your Perfect Empty Backbone

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    Blog Post

    ...with the proper selection marker or antibiotic resistance. Next, consider the expression level of your ...

15. Advice for Choosing a Research Project

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    Blog Post

    ...to use to reach these milestones? Can you get assistance from people who have the required expertise to...

16. The Stingy Scientist: How the Baby Gel Box Was Born

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    Blog Post

    ...before the advent of techniques like PCR and DNA synthesis. Most molecular biologists bought the pricey stuff...

17. Virus Protocol - Generating Stable Cell Lines

    Type

    Protocol

    ...lentiviral vectors deliver mammalian antibiotic resistance (e.g., puromycin, blasticidin), which enables...not all lentiviral vectors deliver antibiotic resistance. This protocol was established using 293T cells...transgene expression. As polyclonal populations of resistant cells start proliferating and the individual wells...

18. Plasmid Cloning by PCR (with Protocols)

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    Protocol

    ...molecular cloning consist of: Leader Sequence: Extra base pairs on the 5' end of the primer assist with restriction...selecting restriction sites, you should use a DNA analysis tool, such as Addgene’s Sequence Analyzer , to...

19. Viral Vectors 101: Transductions

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    Blog Post

    ...non-integrating. Read on to find out more! Molecular basis of transduction Transductions utilize the ‘normal...

20. Hot Plasmids and Viral Preps - September 2021

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    Blog Post

    ...selection marker, Thy1.1, in addition to puromycin resistance.  The MYC-CRISPR library targets E-boxes genome-wide...