.... Digestion with enzyme “B” detects primer multimerization (purple) which is evident by an increase in...manufacturers’ PCR protocol. Be sure to adjust the extension time to match the size of your template. Amplification...CRISPR-induced genome modifications. Occasionally, multimerization of the PCR primers can cause duplication of...

...tweets from the Symposium under #FORBOS15) - my first time live tweeting: a fun and surprisingly exhausting...why they are paid relatively low wages (Stephan estimates that postdocs are paid $16.15/h before fringe ... is certainly true that not all PIs can take the time to do this, you should always reach out to your ...

... IF, where one target is labeled and imaged at a time, inactivating the fluorescent secondary antibodies...antibody may be binding non-specifically – try optimizing your blocking and washing conditions or consider...-reactivity. Keep in mind that you may need to optimize the conditions and concentrations of all the different...

...that sometimes it is necessary or desirable to control or look at multiple genes at the same time.  The...transcription of a target gene, it therefore determines the timing of gene expression and largely defines the amount...

...same pace and enter the resolving gel at the same time where they are then separated by size. Gradient ...concentrations, blocking buffers, and incubation times. If the vendor includes publications that cited ...   Analyzing the western blot Once visible, it’s time to analyze your protein of interest. Analysis typically...

...evidence of this, you needn’t look any further than testimonials from the students who participated in the program...mentee’s personality and professional needs at any time. In its Pilot Program 2014-15, IMP provided mentorship... monthly progress video conferences and had real-time correspondence via e-mail throughout the entire ...

...silencing efficacy, thereby requiring a substantial time investment. Once the target mRNA is cleaved, the...and robust gene silencing (following initial optimization of the amiRNA). Although downstream work is ... manually extracted. This process is tedious and time consuming, as RNAi mutants with a reduced growth...

...SNAP-OMP25 with Atto590-BG (green), imaged with stimulated emission depletion (STED) nanoscopy. Scale bar...updating your labels easy! Instead of taking the time to clone a new construct for every new-and-improved...switching between different colored ligands at different time points.   Figure 3: Single-molecule tracking...

...of protein function. In addition, the level and timing of expression can also be affected by the tag.  ...immune complexes will no longer recognize them. Sometimes, users will combine methods and fix with formaldehyde...health and limit debris, keep samples on ice at all times and use gentle pipetting instead of vortexing. Include...

...a large majority of neuronal cells are labeled overtime due to the continued replication of the virus....have been widely used by neuroscientists for some time due to their advantageous characteristics for brain... titration on the Addgene website).  Tissue Optimal Serotype CNS AAV1, AAV2, AAV4, AAV5, AAV8, ...

...It’s a very thankful time of year for those of us at Addgene! And we can’t think of any better way to...Addgene blog? We'd love to hear from you! Email us anytime at [email protected]. ...

...Plasmid technologies are constantly evolving, but sometimes a technology is so useful it forever enhances ....G This plasmid has been requested nearly 6,000 times (over 800 orders in 2017) and expresses the VSV-...

... have amazing stories to share. I spend a lot of time telling scientists about Addgene’s repository, and... and request plasmids, but I also spend a lot of time listening. I’ve had hundreds of conversations with...

...Sleigh bells, please! It's time to announce the winners of the 2022 #DeckTheLab contest!  and it's.....up your pipettes, and seal up those plates – it’s time to turn your attention to decorating! To join the...

...world. In the years since, our scientists have optimized many steps in the viral production and quality...virus-related protocol videos that may help to save you time and improve your experiment results! Subscribe to...

...Listening! Click here to listen! Podcast breakdown: (Time : Topic) 0:00-1:00 : Introduction 1:01-9:04 : What...We'd like to thank Professor Cepko for taking the time to talk to us and look forward to hear about more...

...of gene drives. Listen here! Podcast breakdown (Time : Topic) 0:00 - 2:00 : Introduction to the podcast...outreach. Many thanks to Alex Chavez for taking the time to sit down and talk with us.  Alejandro (Alex) ...

...to find published plasmids? Do you spend a lot of time in PubMed searching for research articles? Well ...list” to the left of your screen. That’s it! Next time you log-in to your PubMed account and are browsing...

... The team with the best costume takes home the ultimate prize – a glorious trophy. Sure it’s plastic and... changing seasons – and they also invited Father Time to the party. They won over our judges with this...

...download their plasmid request data, as a CSV file, anytime. The CSV is a rich source of information and has...of requested plasmids, or trends of requests over time. Visualizing the data So, after downloading my plasmid...