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We narrowed to 1,076 results for: TOR;

Showing: 541 - 560 of 1076 results

  1. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...with CRISPR–Cas nucleases, base editors, transposases and prime editors. Nat Biotechnol 38:824–844 . https... are even more exquisite for the so-called base editor Cas9, which makes DNA changes in the absence of...breaks (Rees et al., 2018). For C>T and A>G base editors, the intended edit must be in a 5 - 10 nt window... guest blogger John Doench! John Doench is the Director of R&D in the Genetic Perturbation Platform at...

  2. A Control for All Seasons

    Type
    Blog Post
    ...studying the effect of a new tau protein kinase inhibitor on slowing diseases progression in an Alzheimer..., the experimental group, with the new kinase inhibitor and a second group, the positive control group...protocol but does not receive either protein kinase inhibitor. Since the negative control group does not receive...If one were studying the effects of tau kinase inhibitors on mice, for example, it would be better to treat...available materials, lethality of knockdown, or other factors, consider using one of the alternative, albeit ...

  3. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    ...and viral vectors containing genetic elements from rabies plasmids to international requestors. We also...rabies materials! Some of the materials in our repository, including rabies plasmids, are export-controlled...approved, allows Addgene to ship plasmids or viral vectors containing sequences from rabies and all other ...

  4. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    ...Joanne Kamens, Addgene’s executive director, said during our celebratory lunch, it’s hard to picture one ... the scientists who deposited plasmids to the repository and for the scientific sharing community (check... in 2016 and we just reached our 10,000th viral vector shipped! In the upcoming months, we will be moving...

  5. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    ...ground and what the founders hope to see in the repository’s future. If you’ve ever been curious about what...be successful and the importance of lentiviral vectors in ensuring that success Chapter 6 - Developing...distribution!) Chapter 8 - Opportunities to create repositories with other materials Chapter 9 - The Founders...computer systems and networks. Benjie was Addgene's Director of Technology for 7 years, and is now Chief Software...

  6. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    ...? 11:51 - 13:28 : How do you define what a good mentor is? 13:29 - 14:31 : What drives you to do science...the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research Looks to Improve... mentorship... mentor...

  7. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...including vector-insert joining for recombinant plasmid construction (restriction cloning), adaptor ligation...reliably detect single-base differences. The original creators of this technique visualized the ligation product...specific base sequences, but are sensitive to distortions in helix shape (22). Large purine:purine mismatches... the Thermostable Ligase Reaction Temperature Calculator can be used to estimate an appropriate incubation...

  8. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...Meselson-Stahl, Hershey-Chase...Who was I to dispute history? And boy did I make the right decision. If I’d ...kit creation and quality control efforts with depositors; Gizela, the lab member who knows how the physical...responsible for guiding new deposits into the repository, performing quality control, and providing technical...Jefferson dined alone.” There was a time in human history when an intelligent, motivated person really could...it alone. The help of fellow lab members and collaborators will not only get your paper out the door much...

  9. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...your community, think hard about picking a good mentor, and begin considering career options early. Tune...Depace's Talk on "Precision and Plasticity in Mentoring" We'd like to thank Niroshi and Ben for taking...the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research Looks to Improve... mentor...

  10. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ... the party going! At Addgene, we’ve set-up an exhibitor booth in order to help scientists find the plasmids...benefit from depositing their constructs to the repository, and getting feedback from our community. It ...Zhang was awarded one of this year’s SfN Young Investigator Awards and we couldn’t wait to congratulate ... to help their labs save time and resources by storing and distributing their plasmids with the scientific...

  11. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...editing (SECURE) base editors Keith Joung’s lab recently found that a cytosine base editor (CBE) with rat APOBEC1... tethering of DNA template to Cas9 SECURE base editors Nme2Cas9 CRISPR interference in Candida albicans...Read the paper in Nature Find the SECURE base editors All-in-one AAV delivery of Nme2Cas9 with gRNA ...

  12. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ...resources. In 2022 alone, our website saw 2.2 million visitors from 231 countries. Guides and protocols were ...beloved blog received 1.3 million page views from visitors in over 200 countries. We, like everyone around...have many successes to reflect upon, a wealth of history to learn from, and a compelling future to plan ...forward to working with Addgene colleagues, collaborators, partners, and the larger scientific community...

  13. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    ...published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates. Scientists must do science ...in the Netherlands. There are many academic administrators who are finding ways to provide new types of...designing career exploration programs or peer mentoring groups. But for now, you can click here to register...October 2-3, 2014 Boston University Jacob Sleeper Auditorium, Boston, MA Register on Eventbrite (This is a...

  14. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

    Type
    Blog Post
    ...the same page with the potential PI in terms of mentoring style and expectations. Make sure you will enjoy... Tsang, Science Communications and Marketing Coordinator Networking “Many, perhaps most of the most interesting...about what's out there.”- Joanne Kamens, Executive Director Career exploration “You can't wait for opportunities...my team at Addgene was created.”- Eric Perkins, Director of Science Product Management “I knew very early...

  15. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ... Best Way to Elute and Store Your Plasmid DNA? by Paolo Colombi Should I store my plasmid in TE, Tris...posts on COVID-19 featuring plasmid tools from depositors who quickly shared their reagents: detection ...expression, and more. We also highlighted reagent repositories and other collaborative efforts in the scientific...

  16. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...   Alexa Fluor 700APC-H7APC-Cy7   Many factors can influence the level of brightness beyond the...detected through a cell surface label. The viral vector also encodes for eGFP, which serves as a transduction...example, point at issues with the double-expression vector.) In our flow experiment, we'll want to identify.... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity... Guide Ready-to-use antibodies in the Addgene repository Antibody Protocols ...

  17. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    ...member of the Addgene Advisory Board, and Associate Director of the Genetic Perturbation Platform at the Broad...per gene. Further, the pools are available in two vector formats: all-in-one (lentiCRISPRv2, expressing ...both Cas9 and an individual gRNA from this single vector) and guide-only (lentiGuide expressing only an ...blogger, John Doench! John Doench is the Associate Director of the Genetic Perturbation Platform at the Broad...

  18. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...other researchers, and cloned them into lentiviral vectors to generate a kit which is now available at Addgene...cDNAs into a single consensus lentiviral expression vector and attached a DNA barcode to each to make the ...environments, for example. “We’ve been contacted by investigators all over the world who are interested to use...researchers have plans to expand the library to include activators for more cancer-related pathways and more nodes...

  19. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...yeast. These tagging vectors are based on previously described pFA6a-link vectors and include a Kan, SpHIS5...from these yeast-optimized fluorophore tagging vectors for your single or multi-color imaging experiments...protease site)-6xGly linker-(epitope tag)-ADH1 terminator-(selection marker). Additionally, Dr. Formosa...

  20. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    ... created the IBEX Knowledge-Base, a community repository and resource for spatial biology enthusiasts....includes 1049 community-validated reagents from 20 contributors, including 61 fluorescent probes, representing... showing the IBEX Knowledge-Base, with every contributor listed as an author.  The IBEX Knowledge-...Imaging Community’s vision is an open, global repository for reagents, protocols, panels, publications...
Showing: 541 - 560 of 1076 results