We narrowed to 1,073 results for: TOR;

...expression of the protein. However, expression of receptors may not need to be as high to achieve an observable...titer may not need to be as high when delivering receptor constructs with AAV. In the case of channelrhodopsins...may not be necessary to concentrate this type of vector to such a high titer when looking for effective... possible, use low-bind or siliconized tubes to store samples as it is more difficult for viral particles... advances in recombinant adeno-associated virus vector production." Kidney international 61.1 (2002): ...Where do they come from and what do they mean? AAV Vector Quality Control: Going the Extra Mile with NGS ...

...Addgene depositors have made it easier to track translation in two different ways: by monitoring the first...complexes. Recent work has adapted this technique to monitor local translation and determine if proteins are...fusions to their reporter mRNAs. Using translation inhibitors cycloheximide and puromycin, Halstead et al. ...view for constant tracking. You can then directly monitor multiple rounds of translation from this single... Yan et al. used harringtonine, a translation inhibitor that stalls ribosomes after initiation, to calculate...

...lentiviral preps of select lentiviral vectors from the repository for you. We perform test infections with...easy process. The cloning capacity of lentiviral vectors (~8.5 kb of insert) and the reduced titer associated... your gene of interest with a variety of Cas9 activators available in lentiviral format. Finally, while...lentiviral format Another popular set of tools in the repository are CRISPR pooled gRNA libraries that can be ...Page Check Out Our CRISPR Guide Pages Find Viral Vectors ...

...devices to our own interests with the content we store in them: music, emails, chats, pictures, and apps...culture and include a counter, lab timer, dilution calculator, and a couple of other nice features. This app...sequence for your enzyme of interest. Lastly, the Tm calculator helps you set up an optimal PCR depending on ...information in the app is restricted to the NEB inventory only, the restriction enzyme sequence resource...acids memorized, but the structures are another story. AMINO has been very helpful when I’ve had to explain...apps: Findings Lab Notebook Cell Culture Split Calculator by DRDP Restriction Enzyme Tool by Promega Material...

...contributed by guest blogger Jessica Polka, a Postdoctoral Research Fellow with Pamela Silver.  Most types...enormous lattice of identical subunits. A brief history of R bodies R bodies have been studied for many...bodies do for you? R bodies are robust linear actuators that could be used in a variety of applications...applications, purified R bodies could also perform as actuators in microelectromechanical systems (MEMS) devices...with their environment.   Jessica Polka is a Postdoctoral Research Fellow with Pamela Silver in the department... Biology Posts  Suggest Plasmid Tools for the Repository Resources on Addgene.org Read our Hot Plasmids...

...resources at Addgene. His background in history and specifically the history of RNAi left him a bit skeptical...to the Advisory Board. John Doench is Associate Director of the Genetic Perturbation Platform at the Broad...Listen to the interview here! Doench majored in history during his undergraduate work at Hamilton College...targets and what confers specificity in the miRNA regulatory process. This early research is still relevant...function really…. Addgene’s role as a central repository for what is useful and what was used once and...

...have perfected processes to curate the data our depositors share and enrich it with sequencing data; as ...categories — catalog, educational resources, and depositors — each with subcategories such as plasmids and...educational resources, and labs and institutions for depositors (see below). Even if the user experience is similar...scoped to plasmids shows filters for expression and vector type, while a search scoped to viral preps shows... match. For example, matches of a name in the depositor field will rank higher than matches of the same...that contains a plasmid with the catalog ID). Depositor suggestions provide shortcuts to matching PI labs...

...define what type of factor you are looking for and what process or outcome that factor functions in. The...there is removal of known factors (such as a genes) and the loss of those factors is assessed. The two most... committed to the daunting task of finding new factors in your biological system of interest, the so-called...selected growth conditions. For screens, there is no obligatory growth advantage – that is, you are not specifically...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles... cassette provides increased simplicity for combinatorial genetic screens, and this optimized toolkit ...50 mLs) or through two polycistronic expression vectors (3570 lanes/50 mLs) coexpressing a set of either...by: Rachel Leeson The large size of CRISPR-Cas effectors and fusion proteins, such as Cas9 and Cas12a, ...by the fusion of Cas12f with a transcriptional activator, CasMINI is able to induce high levels of gene...packaged in AAV. A dCasMini-mediated adenine base editor allows for base editing, while gene editing can...

...cerevisiae expression vectors are available at Addgene. A set of mammalian expression vectors containing various...expression using either TEF1 or GAL1 promoters. These vectors are intended for the high level expression and ...spike protein) cloned into a CMV driven expression vector are also available. These contain no tags and are... genes in E. coli and S. cerevisiae expression vectors in late March. The first set of 383 constructs ...status we call “Archived.” This status allows us to store plasmids and make them available to scientists without...

...this list, that does not necessarily mean the repository does not have it. That said, if you know of a... don’t hesitate to start the process! Addgene depositors who study chromatin The PIs linked below are ...Chromosome Organization. These aren’t the only investigators in the chromatin field who have deposited with...Addgene website. In-exhaustive List of Addgene Depositors Who Study Chromatin Cheryl Arrowsmith James...SNF-like BAF complex to chromatin after T lymphocyte receptor signaling. Zhao K, Wang W, Rando OJ, Xue Y, Swiderek...Bystricky, Ben Martin, and Berkley Gryder. Addgene depositors or not, your time is valuable ...

...textbook, a laboratory kit, and support during the academic year from remote scientist mentors and the BioBuilderClub...effectively contribute as part of a team.” As a seasoned educator, Kuldell also knew that the best days in the classroom...meetings and newsletters, pairs teams with STEM mentors, and provides a nationally recognized platform ...state-of-the-art lab embedded in LabCentral’s community of innovators that opened in August 2017.  This flexible teaching...BioBuilder! Mary Tamer is a Boston-based writer, editor, and consultant whose work is focused on education...

...-introduced breaks are repaired. The following factors are required for NHEJ repair regardless of end ...XRCC4-ligase IV); together with some accessory factors (PAXX, XLF), this complex holds a pair of DNA ends...Accordingly, NHEJ has a vast toolbox of processing factors, including polymerases (Pol μ and Pol λ), nucleases... sequence (“microhomologies”). NHEJ doesn’t obligatorily introduce indels. Given the end structure of...majority of their chromosomes within a day. Another factor expected to impact repair is that the Cas9 protein... loading of Ku and normal NHEJ activity. Other factors can also influence NHEJ activity. For example, ...

...pluripotent stem cells: a transcription activator-like effector nuclease (TALEN) kit consisting of 834 ...stem cells. Addgene: Tell me about the history. How had investigators done this kind of work before? Musunuru...Unfortunately, it is time consuming and difficult for investigators without a lot of expertise to create zinc finger...and puts it in the hands of pretty much all investigators. Our kit makes it extremely simple to design...

...already run several reproducibility workshops as Director of Scientific Outreach at Code Ocean and through...well received, and then when the team started brainstorming ideas for the next workshop, they realized that...workshop at the conference, but because each contributor made the materials accessible, anyone could present...and were presented at the ASPB meeting by the collaborators who could participate. Despite the group not...visibility of these materials, Sonali Roy, a postdoctoral researcher at the Noble Research Institute, ... Schwessinger for helpful conversations on the history of the group, lessons learned, and to Sonali Roy...

...there are many factors that interact with sex, and it’s important to analyze these factors during an experiment...blogger Londa Schiebinger, PhD, Hinds Professor of History of Science, Stanford University. Sex and gender...experiment. In the case of stem cells, these factors may include cell type, the disease being treated, ...other hormonal, immunological, and environmental factors. How to include sex in experimental design Recognizing...variable in all NIH-funded research. Janine Clayton, Director of the Office of Research on Women’s Health, emphasizes...

...ITRs), hairpins, or strong terminators. Detailed instructions on other factors to consider when designing...community. This is no small task, however, at a repository that consistently receives around 200 new DNA...are sequencing from the exact same stock that we store and distribute from. Of course, your culture should...overnight cultures for glycerol stock production and storage, we grow a little extra and transfer 100 µl of ...Pages Get Plasmid Backbone Information from Our Vector Database  ...

...engineered prime editors to expand their PAM flexibility. Find the engineered prime editors here A lentiviral...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...pubmed.ncbi.nlm.nih.gov/24390935/ Hypercompact genome editor CRISPR-Casɸ By Melina Fan The Doudna Lab adds to...discovery of CRISPR-Casɸ, a hypercompact genome editor from the Biggiephage clade of huge phages. In this... Scientists looking for more compact genome editors due to delivery constraints and scientists seeking...

...Acr) proteins are phage-derived small protein inhibitors of CRISPR-Cas systems that help phages evade ...the best time to shut off Cas activity? Using inhibitor timing experiments, the Doudna lab found that ...also be used to reduce off-target edits of base editors, which convert a nucleotide base pair to another... means they can be delivered in vivo using AAV vectors. Having a way to shut off CRISPR-Cas systems prevents...Maxwell KL, Doudna JA (2017) A Broad-Spectrum Inhibitor of CRISPR-Cas9. Cell 170:1224-1233.e15 . https...Papayannopoulou T, Lieber A (2018) HDAd5/35++ Adenovirus Vector Expressing Anti-CRISPR Peptides Decreases CRISPR...

...ask the depositing laboratory to review the discrepancies. If the depositing laboratory confirms that these...reference sequence or a sequence provided by the depositor. Depositing labs often provide insert sequences... actin promoter, and rabbit beta-globin splice acceptor, contains sequence that is over 80% GC. Some IRES...information about our sequencing results in the Depositor Comments section at the bottom of the plasmid ...hope to make available as part of our OpenBio Repository sometime soon. Stay posted!     References ...