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We narrowed to 797 results for: Tec

Showing: 541 - 560 of 797 results

  1. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...use DNA sequencing or other molecular cloning techniques to determine CRISPR/sgRNA efficiency of an experiment...editing, regulating and targeting genomes. Nature Biotechnology 32:347–355 . https://doi.org/10.1038/nbt.2842...

  2. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...Tim Miles, PhD, Director of the CLOVER Center at CalTech? If so, you may have submitted a question that ...with no more ado, the questions!  AAV Prep and Technique Q1: Is there any way to deliver a long gene (>...

  3. Clear and Accessible Writing

    Type
    Blog Post
    ...most helpful to use a lot of jargon and have a technical, detailed approach. Other times, it is more helpful...thought it would be most helpful to highlight the technical details of collecting the purified AAV–that the...

  4. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...also activating or inhibiting gene expression, detecting neuronal signaling, or manipulating any other ...Others have noted a lack of transduction in catecholamine pathways from the spinal cord (Wang, 2018, Metcalfe...

  5. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...restriction modification systems that prokaryotes use to protect themselves from foreign DNA. The other component...Addgene Blog Keep up with the Latest Cloning Techniques Learn to Troubleshoot Your Sequencing Results...

  7. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...overview of general molecular biology knowledge and techniques. If you are interested in reading more, you can...Addgene Website Choose the Best Molecular Cloning Technique for You Browse Addgene's curated list of Bacterial...

  8. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...involved “plates,” and our scientists and lab technicians always knew from context what a “plate” was. ... the lab to be using words for their field or technology in a consistent way. Just creating a lexicon ...

  10. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...the steps you will have to take. Decide which technique you are going to adopt (i.e. restriction cloning...Find how-to videos for molecular biology and lab techniques ...

  12. dTAG - You're it!

    Type
    Blog Post
    ...exciting strategy, it is difficult to apply this technique to oncoproteins that lack selective small molecule...author on the recent paper describing the dTAG technology. Follow him on twitter @behnamnabet.   References...

  13. RNA Extraction Without A Kit

    Type
    Blog Post
    ...laboratory situation or tissue collection method, so biotechnology companies have marketed several products that...These products, namely RNAlater®  from Thermo and RNAProtect® from Qiagen, are ammonium sulfate-based solutions...

  15. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ... will not recognize a Fab fragment. In order to detect a Fab fragment, it either needs to be directly ...identified by a double-label immunofluorescence technique for monoclonal antibodies.  J Histochem Cytochem...

  16. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ... the open windows in the middle of your talk. Technical difficulties turn your slide deck into a chalk...everyone! Everybody makes mistakes, encounters technical difficulties, and runs into unexpected stumbling...

  17. To Each HIS Own

    Type
    Blog Post
    ...Proteins with a Novel Metal Chelate Adsorbent. Bio/Technology. 1988; 6: 1321–1325. Janknecht R, de Martynoff...growth factor I from culture medium of E.coli. Bio/Technology. 1987, 5:379-382. Bornhorst JA and Falke JJ. ...

  18. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...error rate of current NGS approaches allows for a detection limit of roughly 0.1%. Thus, rare, pre-existing...prior to treatment, there are small (previously undetectable) populations of cancer cells that are resistant...

  19. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...hexon proteins. The viral genome DNA is further protected by histone-like proteins forming the nucleocapsid... assays are commercially available for faster detection of RCA. 2nd Gen AdVs were designed to have increased...

  20. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...control. But even with the advent of CRISPR/Cas9 technology, inserting large tags into a precise location...plasmid into the cells we use electroporation, a technique that briefly destabilizes the cell’s outer membrane...
Showing: 541 - 560 of 797 results