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  1. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...introduction of DNA into bacterial cells); however, the techniques and reagents vary. Plasmid transfection into ...positive selection; however, negative selection techniques can be used in conjunction with positive selection...

  2. Clear and Accessible Writing

    Type
    Blog Post
    ...most helpful to use a lot of jargon and have a technical, detailed approach. Other times, it is more helpful...thought it would be most helpful to highlight the technical details of collecting the purified AAV–that the...

  3. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...use DNA sequencing or other molecular cloning techniques to determine CRISPR/sgRNA efficiency of an experiment...editing, regulating and targeting genomes. Nature Biotechnology 32:347–355 . https://doi.org/10.1038/nbt.2842...

  7. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...Without these scientists and the support of their technology transfer offices, Addgene would not be able to...educational materials, and provides viral vector technical service (details about virus production protocols...

  9. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...Tim Miles, PhD, Director of the CLOVER Center at CalTech? If so, you may have submitted a question that ...with no more ado, the questions!  AAV Prep and Technique Q1: Is there any way to deliver a long gene (>...

  10. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...also activating or inhibiting gene expression, detecting neuronal signaling, or manipulating any other ...Others have noted a lack of transduction in catecholamine pathways from the spinal cord (Wang, 2018, Metcalfe...

  11. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...restriction modification systems that prokaryotes use to protect themselves from foreign DNA. The other component...Addgene Blog Keep up with the Latest Cloning Techniques Learn to Troubleshoot Your Sequencing Results...

  13. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...overview of general molecular biology knowledge and techniques. If you are interested in reading more, you can...Addgene Website Choose the Best Molecular Cloning Technique for You Browse Addgene's curated list of Bacterial...

  14. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ... will not recognize a Fab fragment. In order to detect a Fab fragment, it either needs to be directly ...identified by a double-label immunofluorescence technique for monoclonal antibodies.  J Histochem Cytochem...

  16. RNA Extraction Without A Kit

    Type
    Blog Post
    ...laboratory situation or tissue collection method, so biotechnology companies have marketed several products that...These products, namely RNAlater®  from Thermo and RNAProtect® from Qiagen, are ammonium sulfate-based solutions...

  17. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...involved “plates,” and our scientists and lab technicians always knew from context what a “plate” was. ... the lab to be using words for their field or technology in a consistent way. Just creating a lexicon ...

  19. dTAG - You're it!

    Type
    Blog Post
    ...exciting strategy, it is difficult to apply this technique to oncoproteins that lack selective small molecule...author on the recent paper describing the dTAG technology. Follow him on twitter @behnamnabet.   References...

  20. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...the steps you will have to take. Decide which technique you are going to adopt (i.e. restriction cloning...Find how-to videos for molecular biology and lab techniques ...
Showing: 541 - 560 of 796 results