... responsible for establishing a new report and routine across the marketing and content team. Initially...

... induce?  Fc receptors are intriguing from a therapeutic standpoint. Engineered monoclonal antibodies ...

...proficient and deficient for the target. CRISPR has revolutionized the generation of knockout cell lines which...

... you’re interested in (i.e. biotechnology, pharmaceuticals, etc.) Do their jobs sound interesting? Is ...

...well known and, as a result, a target of many pharmaceutical drugs, there is still only a partial understanding...

...Since the start of the CRISPR revolution, Addgene has distributed over 100,000 CRISPR plasmids. But that...

...transformation. While restriction cloning is still routinely performed by many labs, a variety of other cloning...

... to find out more about our free deposit and distribution service! Did you see a must-have plasmid during...

...take the initiative by bringing and effecting solutions to both known and unidentified issues. There are...

...expression at an opportune time or go all out with constitutive high level expression? Finally, you can add ...

... your fingers in during a run or risk minor electrocution.  These days you can buy small gel boxes, but...

...stable in powder form, but quickly breakdown in solution. Storing aliquots at -20oC and avoiding repeated...

...in the dilution BSC. Prepare 1X dilution buffer (see recipe above). Save ~25 µL of 1X dilution buffer ... carefully add the dilution buffer to the dilution plate according to the dilution scheme listed in step...Pro-Tip Prepare the viral dilutions in a separate biological safety cabinet (dilution BSC) than that used for...touching the screen. Prepare the Serial Dilution Place a 48-well dilution plate in a chilled 96-well freezer... add 5 µL of each viral sample to Dilution 1 in the 48-well dilution plate and pipette 5–10 times to mix...change tips each time. Dilution 1 (20X): 5 µL in 95 µL 1X PCR buffer (1:20) Dilution 2 (20X): 5 µL in 95 ...PCR buffer (1:400) Dilution 3 (20X): 5 µL in 95 µL 1X PCR buffer (1:8,000) Dilution 4 (20X): 5 µL in 95...

... Molecular basis of transduction Transductions utilize the ‘normal’ mechanism of viral infiltration into...

...cabbage and kale to name a few. Despite Arabidopsis’ utility to science, and its well-known edible relatives...

...respond to blue light. We recently released a constitutive (pAAV-Syn-Archon1-KGC-GFP-ER2) and cre-dependent...

... on another cell or organism. This pipeline is utilized to transduce cell lines for basic science research...

...instability can cause partial loss of an ITR during routine plasmid propagation, with complete ITR loss abolishing...

...scripts with laboratories and viral vector cores routinely producing AAV. We believe that this simplified...

...to expand upon the work and continue to make contributions towards this COVID-19 pandemic,” says Balazs...