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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 750 results for: Uty

Showing: 541 - 560 of 750 results

  4. Lentivirus ddPCR Titration

    Type
    Protocol
    ...transfer 200 µL of the first dilution into the next dilution. Repeat for all dilutions as indicated in the chart... virus dilutions were used to transduce cells: Initial dilution factor of virus stock Dilution factor ... µL 5 200 µL of 2.5-fold dilution 200 µL 400 µL 10 200 µL of 5-fold dilution 200 µL 400 µL 20 200 µL of... of 10-fold dilution 200 µL 400 µL 40 200 µL of 20-fold dilution 200 µL 400 µL 80 200 µL of 40-fold dilution... 160 200 µL of 80-fold dilution 200 µL 400 µL Add 150 µL of each viral dilution to a well of a 6-well ... viral dilution). Remember, this is an additional 10-fold dilution of the virus stock! Don’t forget to...~2 as you progress from the higher dilutions to the lower dilutions (blue). As shown in the image (Figure...

  5. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...EDTA Store Solution I at 4°C Solution II - Denaturing Solution 0.2 N NaOH 1.0% SDS Store Solution II at room...plasmid Resuspension buffer Denaturing solution Renaturing solution 2 mg/mL RNase A TE or water-saturated...that is compatible with the next solution. Add a denaturing solution to the resuspended bacteria. Note...contents, including plasmid DNA, into solution. Add a renaturing solution to the denatured bacteria. Note: ...Lysis Plasmid Miniprep Prepare the following solutions: Solution I - Resuspension Buffer 25 mM Tris-HCl (pH... room temperature Solution III - Renaturing Solution (Potassium Acetate) 120 mL 5M Potassium acetate 23...Resuspend the pellet in 100 μL of cold Solution I. Vortex the solution for 2 min or until all bacteria are...
Showing: 541 - 560 of 750 results