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  1. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...homology arm, but keep in mind these numbers may vary based on your locus of interest and experimental...ssODNs in mouse models. Although HDR efficiency varies across loci and experimental systems, ssODN templates...

  2. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...and red fluorescence. The paper reports a wide variety of applications of the Enhanced Nano-Lanterns including...PMC4942401. 5. Susuki, K., et al. (2016) Five colour variants of bright luminescent protein for real-time multicolour...

  3. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...to the intended edit rather than some passenger variant that came along with the single cell clone (although...SaCas9, NmeCas9, Cas12a enzymes, and engineered variants thereof offers additional PAM options that can...

  4. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...demethylation and induce transcription at CpG regions of various promoters. Other researchers additionally fused...applications direct catalytically dead dCas9 fused to a variety of epigenetic modifiers to specific loci without...

  5. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...responsive to light in combination with retinal) variations designed to give researchers flexibility in using...photoswitches can be used in a single pathway to activate various protein regulators at different points in the pathway...

  6. Finding Your Science Policy Path

    Type
    Blog Post
    ... worked for seven different organizations in a variety of science policy roles including fellowships, ...folks who work in science policy come from a wide variety of backgrounds with technical expertise that ranges...

  7. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...curing diseases caused due to single nucleotide variations in the genome.    Due to the rise of CRISPR in...engineering and open advertisement of the technology on various non-scientific platforms, CRISPR has also transcended...

  8. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...investigator (PI) Runs the lab Writes a lot of grants Varying degrees of engagement with what happens in the ...Undergraduate student Roles and responsibilities can vary "Lab assistants" usually wash glassware, prep media...

  10. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...et al. A percentage is not listed because many variables (cell type, criteria used, etc.) can effect reprogramming...insertional mutagenesis and random integration creates variation between iPSC clones. Retroviruses are not considered...

  11. Cloning

    Type
    Guide
    ...fusing it with different tags, putting it under a variety of promoters and into backbones with different ...vector yourself. Additionally, the efficiency can vary depending on the polymerase used, and the single...

  14. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...thoroughly before use.  For efficient mixing, add the various reagents to a single tube and simply flick the ...
Showing: 541 - 560 of 767 results