Using AAV for Neuronal Tracing
Type
Blog Post
...mammalian brain (Wickersham et al., 2007; plasmids at Addgene).
AAV vectors: A platform for delivering neuronal...their capsid proteins. For a brief overview see Addgene’s AAV guide.
AAV particles are relatively small ... production, purification and titration on the Addgene website).
Tissue
Optimal Serotype
CNS
AAV1...Serotypes and their target tissues (adapted from Addgene's AAV guide)
Commonly used serotypes include AAV1...learning and memory (Kohara et al., 2014; plasmids at Addgene).
The Gradinaru Lab at the California Institute...the brain based on its unique hue (plasmids at Addgene).
A large-scale example of neuronal tracing with...other AAV serotype (Tervo et al., 2016; plasmid at Addgene). Its efficiency is comparable to conventional ...