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  1. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ...Children’s Hospital and Harvard Medical School. Addgene is proud to present a video reprint of the CRISPR...the full protocol from the JOVE article here on Addgene's website and find more CRISPR resources.    Thank...Browse CRISPR Plasmids CRISPR Information at Addgene More CRISPR-Cas Blog Posts    ...

  2. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    ...and Dr. Emmanuelle Charpentier. I was joined by Addgene Scientist Matt Ferenc (one of our resident CRISPR... to a year or greater with older technologies. Addgene is proud to be helping get CRISPR reagents out ...  New to CRISPR/Cas9 Technology? Learn More: Addgene's CRISPR/Cas9 Guide History of CRISPR Cas - A tale...

  3. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    ...EikeMahlandt @AnoeskaMoosdijk @Saskia_deMan @SILS_UvA @addgene #SciArt #fluorescence #LCAM #DeckTheLab pic.twitter.com...holiday season. Santa frog is coming to town. @Addgene @UMich_MCDB #christmastaco #frogsnowflake #xenopus...

  4. Twenty Years of Sharing Science!

    Type
    Blog Post
    ...Today marks Addgene's 20th anniversary! We've done so much in the past two (2!) decades that it's hard...publication by pasting the paper's title into the Addgene website search bar?   We'll be celebrating all ...protocols, and of course, the friendly folks at help@addgene.org! Fun fact! Did you know you can search for plasmids...

  5. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...resources on Addgene.org IPI Antibody Collection Antibody Guide Addgene Antibody Collection Addgene Antibody...especially when sharing with other scientists. Addgene partners with IPI to provide recombinant antibodies...data from IPI and other labs easily viewed in Addgene’s Data Hub. The diversity of antibody reagents is...button_text" is_json="true" %}{% raw %}"Visit Addgene's Data Hub"{% endraw %}{% end_module_attribute %...0471142727.mb0803s51 Additional resources on the Addgene blog All Antibody Topics Antibody Data Repositories..."href":"https://datahub.addgene.org/","href_with_scheme":"https://datahub.addgene.org/","type":"EXTERNAL...

  6. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ... of the vector you are using. Contact Addgene (help@addgene.org), or the supplier of your vector, to obtain...Technologies Resources on Addgene.org Find ready to use AAV vectors at Addgene. Find AAV plasmids to use...one of the most common concerns we get here at Addgene about this system is off-target or spurious expression... cells that do not express Cre or Flp. Here at Addgene we quantify the amount of transgene recombination...2.4x10e13 GC/mL) for this study were packaged at Addgene and had a relatively low recombination rate (0.1%.../fnmol.2020.618020 Additional resources on the Addgene blog Plasmids 101: FLEx Vectors Important Considerations...

  7. Finding Your Perfect Job After University

    Type
    Blog Post
    ...perfect job.   Resources at Addgene Read Additional Career Posts on the Addgene Blog Browse Our Careers ...applied anyway. I later found out the job was with Addgene. I had used plasmids from this company before and...also get the chance to contribute content to the Addgene’s Website and travel to meet scientists, making ...develop my own projects, but working remotely (Addgene’s main offices are in Cambridge MA, while I’m based...Careers Page to See What Positions Are Open at Addgene Learn How to Choose a Good Mentor for Your Professional...

  8. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...mammalian brain (Wickersham et al., 2007; plasmids at Addgene). AAV vectors: A platform for delivering neuronal...their capsid proteins. For a brief overview see Addgene’s AAV guide. AAV particles are relatively small ... production, purification and titration on the Addgene website).  Tissue Optimal Serotype CNS AAV1...Serotypes and their target tissues (adapted from Addgene's AAV guide)  Commonly used serotypes include AAV1...learning and memory (Kohara et al., 2014; plasmids at Addgene). The Gradinaru Lab at the California Institute...the brain based on its unique hue (plasmids at Addgene). A large-scale example of neuronal tracing with...other AAV serotype (Tervo et al., 2016; plasmid at Addgene). Its efficiency is comparable to conventional ...

  9. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...Communication Additional Resources on Addgene.org Find Job Openings at Addgene ...started:  Subscribe to Career Blog Posts from Addgene 1) Twitter – Get your voice out there Here’s one...today, Soapbox Science (hosted by Nature), and Addgene are open to guest contributions. If you decide ...BananarootsBlog.   Additional Resources on the Addgene Blog Check out Other Science Communication Posts...

  10. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...generations of lentiviral packaging systems please see Addgene’s Lentiviral Guide. While the third generation packaging... with the unconcentrated prep. For example, at Addgene we typically concentrate 100-fold meaning that ...out the lentiviral protocols and blog posts on Addgene’s website for additional resources. References ...PMCID: PMC4562269. Additional Resources on the Addgene Blog Your lentiviral FAQs answered Tips for titering...viral vector featured topic page Resources on Addgene.org Lentiviral Guide Find lentivirus plasmids Browse...

  11. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    ...Resources on the Addgene Blog Learn about Synthetic Biology with BioBuilder Use Addgene Videos to Learn...School Students Explore Careers at Addgene Resources on Addgene.org Find Yeast Expression Vectors Check...these plasmids, which have been deposited with Addgene, to successfully analyze Met protein complementation...

  12. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...plasmid prepping is a breeze. You get your stab from Addgene, streak for single colonies, sub-culture, and prep...use a different strain. For most applications, Addgene uses and recommends DH5𝝰! This strain consistently... strain instructions from the manufacturer. On Addgene plasmid pages, you may see depositor recommendations...writing of this post. Additional Resources on the Addgene Blog Visit our Plasmids 101 Topic Page Learn How...Lab E. coli Strains Additional Resources on Addgene.org Find Info on How to Quantify Your DNA Learn ...

  14. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    ...Blugene and I represented Addgene at the recent Keystone meeting on Precision Genome Editing with Programmable...desired edit, and I want to make sure that I (and Addgene) don’t forget this in the future. Here’s a list... to keep current with the latest and greatest! Addgene resources 3 Tips to Improve HDR Efficiency for ...

  15. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...you pick the best AAV for your experiment. The Addgene viral service has now expanded to offer many of...pEF1a-DIO-FLPo-WPRE-hGHpA    Additional resources on the Addgene blog Read all of our viral vectors blog posts ...

  16. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    ...When I started writing for the Addgene blog, I was focused on writing about new scientific techniques...first program I used to create graphics for the Addgene blog. However, it has a limited number of drawing...comments section!  Additional resources on the Addgene blog Read our Early Career Researcher Toolbox ...

  17. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    ... Resources on Addgene.org Check out the Molecular Biology Reference Download Addgene's Plasmids 101 eBook...and Khammash's lab have created and shared with Addgene. You can find all the plasmids deposited from Di...s41589-021-00787-6 Additional resources on the Addgene blog Learn more about inducible promoters Visit...

  18. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    ...in flies Resources on Addgene.org Find fluorescent protein plasmids at Addgene Read our Molecular Biology...recombination outcome. Find SPARC plasmids at Addgene To more precisely control the amount of labeling...s41592-018-0184-y Additional resources on the Addgene blog Learn about Gateway cloning Read about genome...

  19. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    ...diversely applied to many different experiments. Addgene was founded in order to store, QC, curate, and ...cloning and gene expression. Just released: Addgene's Plasmids 101 (2nd Ed.) eBook And now for some ..... You can now download the newest version of Addgene's Plasmids 101 eBook! In keeping with our goal of...

  20. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...new to Addgene this year. Feel the love with MTRIA sensors One of the latest additions to Addgene’s catalog...on the Addgene blog Fluorescent Proteins 101: Fluorescent Biosensors Resources on Addgene.org Fluorescent...Here at Addgene’s headquarters, the skies are getting dark and wintry with the end of year approaching...   There are hundreds of other biosensors in Addgene’s catalog — from the recently developed to the tried-and-true...
Showing: 541 - 560 of 1102 results