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  1. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post
    ...where the gene is expressed. Click here to view Addgene’s collection of empty backbones for constructing... such as FRET, calmodulin, and others. Review Addgene's collection of fluorescent biosensors here. Optogenetics...OpenOptogenetics and find optogeneic actuators and sensors at Addgene. Cell marking/selection: Expression constructs ...microscopy. Read More: All Plasmids 101 Articles Addgene's Fluorescent Protein Guide Note: A. Max Juchheim...
  2. CRISPR Cheat Sheet

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    Blog Post
    ...’re bringing you a brand new third edition of Addgene’s CRISPR 101 eBook. This new edition of the eBook...CRISPR nuclease. Note: Most CRISPR plasmids from Addgene produce either a gRNA, a nuclease, or both. Applications...
  3. Antibody Data Repositories and Search Engines

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    Blog Post
    ...Human Cell Differentiation Molecules group Addgene Data Hub Any (see notes) Any Data repository...repository Only accepts data for antibodies in Addgene’s repository  IBEX Knowledge-Base  Any IBEX...
  4. New and Upcoming Viral Vectors - June 2019

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    ...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...These vectors listed below should be released at Addgene in the next 6 months, pending quality control. ....1-WPRE-SV40  Additional resources on the Addgene blog Read all of our viral vectors blog posts ...item is available for order please email help@addgene.org with the vector name and serotype you’d like ...
  5. In Vivo Biotinylation of Bacterial Fusion Proteins

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    Blog Post
    ...the biotin ligase BirA backbone (Eric Campeau, Addgene plasmid #26624) was unsuccessful. Nothing more ...pMal-T-Avi-His/BirA plasmid now available through Addgene. We have successfully used this plasmid to biotinylate...cell signaling.     Additional Resources on the Addgene Blog Check out other Hot Plasmids Find Additional...Posts on Plasmid Technologies Resources at Addgene.org Learn about Popular Plasmids in the Repository...
  6. Management for Scientists: Delegating is Key

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    Blog Post
    ...For example, the many resources available on the Addgene website didn't come into being through the actions... Managerial Skill" Additional Resources on the Addgene Blog Read the Rest of the Management for Scientists... Individual Development Plan Resources on the Addgene Website Are You a Molecular Biologist? Find Plasmids...
  7. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

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    Blog Post
    ...received matches the expected sequence. Here at Addgene, we use NGS-based quality control to confirm the...necessary but also an easy process. Please vist Addgene's resource for plasmid verification to find additional...DNA gel.  More Plasmid Eductional Resources: Addgene's Molecular Biology Reference Find More Protocols...
  8. Plasmids 101: Codon usage bias

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    Blog Post
    ...ordering plasmids containing them from Addgene. If a plasmid at Addgene contains a gene that’s been codon ... for example). As many plasmids available from Addgene now have full sequence data, we recommend directly...PMCID: PMC2840511. Additional resources on the Addgene blog Plasmids 101: protein expression Plasmids...Plasmids 101 featured topic page Resources on Addgene.org Molecular biology reference pages Genetic code...
  9. Antibodies 101: Immunoprecipitation

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    Blog Post
    ...resources on the Addgene blog: Introduction to Immunofluorescence View all of Addgene's antibody posts  ...Resources on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene  ... found this blog helpful be sure to check out Addgene’s blog for other antibody topics.     References...
  10. Fluorescent Proteins 101: Aptamer Fluorophores

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    Blog Post
    ...Resources at Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All ...and used in assays for over half a century; the Addgene blog has an excellent primer and tips from experts..., Pages 1348-1353. Additional Resources on the Addgene Blog Tips for Using FRET in Your Experiments Learn...
  11. Hot Plasmids: Winter 2025

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    Blog Post
    ...contributing to the Addgene Antibody Data Hub.  Figure 5: Immunohistochemistry with Addgene's Anti-APP [...Immunohistochemistry (Human). Addgene Report, doi: https://doi.org/10.57733/addgene.6ncijt.  ...
  12. Great Results Start with Great Standard Curves

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    Blog Post
    ...resources on addgene.org Addgene's Antibody Guide Addgene's Ready-to-Use Antibody collection Addgene's Recombinant...R2 never dip below 0.95! More resources on the Addgene blog Antibodies 101: ELISAs The Four ELISAs and...
  13. Transferable Skills Guide: Career Planning Resources

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    Blog Post
    ... Joanne Kamens Resources at Addgene.org Find Open Positions at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...that person before ever asking them for work. Addgene’s very own Joanne Kamens, a strong advocate for ...Scientists on docjobs.com Additional Resources on the Addgene Blog Check Out Career Topic Page Tips on Writing...
  14. Career Insights: Technical Support Specialist

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    Blog Post
    ...Technical Support Calls Resources at Addgene Visit the Addgene Careers Page Check Out Our Career Videos...post. Klaus Wanisch is an Outreach Scientist at Addgene. These days he's doing a lot of science communication...cellular therapies.     Additional Resources on the Addgene Blog Read What Do I Do Now? Academic v. Non-Academic...this overall rewarding role. Learn about Other Addgenies and Their Careers in Our Career Videos What's ...
  15. To Codon Optimize or Not: That is the Question

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    Blog Post
    ...synthetic? How Addgene;s dataset reveals trends in biological innovation Resources on Addgene.org Check out...silence neurons in vivo (Slimko and Lester, 2003).  Addgene has a large selection of codon optimized genes,...embo-reports/kvd001 Additional resources on the Addgene blog Read more codon usage bias in Plasmids 101...out Addgene's Molecular Biology Reference Read about genetic code expansion Browse our plasmid collection...
  16. Plasmids 101: Multicistronic Vectors

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    Blog Post
    ...released: Addgene's Plasmids 101 eBook And now for some exciting news... You can now download Addgene's new ...depending on the placement of the reporter gene). Addgene's collection offers a variety of plasmids to express...PubMed.   Read more Plasmids 101 posts or browse Addgene's Plasmid Guide for more molecular biology and cloning...
  17. Antibodies 101: The Basics of Western Blotting

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    Blog Post
    ...antibodies Resources on Addgene.org Find written and video protocols Visit Addgene's antibodies collection...plasmid encoding your protein of interest from Addgene, transfected it into your target cells, now what...may even find plasmids encoding antibodies from Addgene that you can make on your own.  To narrow your ... found this blog helpful be sure to check out Addgene’s blog for overviews of other antibody topics. ...10.4103/1947-2714.100998 Additional resources on the Addgene blog Find other tips and tricks for molecular ...
  18. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

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    Blog Post
    ...being deposited at Addgene. Other plasmids and pAAVs can be ordered directly from Addgene. The Podgorski ...References and Resources Additional Resources on the Addgene Blog Learn about other Fluorescent Biosensors ...Fluorescent Protein Featured Topic Page Resources at Addgene.org Find Fluorescent Protein Biosensors Browse All...
  19. Antibodies 101: Monoclonal Antibodies

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    Blog Post
    ...Resource on Addgene.org Check out Addgene’s antibody plasmid collection Read the latest on Addgene's new antibody...www.ncbi.nlm.nih.gov/books/NBK100203/ Additional resource on the Addgene blog Learn more about research applications for...
Showing: 541 - 560 of 1077 results