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Showing: 541 - 560 of 1090 results
  1. Using AAV for Neuronal Tracing

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    ...mammalian brain (Wickersham et al., 2007; plasmids at Addgene). AAV vectors: A platform for delivering neuronal...their capsid proteins. For a brief overview see Addgene’s AAV guide. AAV particles are relatively small ... production, purification and titration on the Addgene website).  Tissue Optimal Serotype CNS AAV1...Serotypes and their target tissues (adapted from Addgene's AAV guide)  Commonly used serotypes include AAV1...learning and memory (Kohara et al., 2014; plasmids at Addgene). The Gradinaru Lab at the California Institute...the brain based on its unique hue (plasmids at Addgene). A large-scale example of neuronal tracing with...other AAV serotype (Tervo et al., 2016; plasmid at Addgene). Its efficiency is comparable to conventional ...
  2. Overcoming the Challenges of Lentiviral Production

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    ...generations of lentiviral packaging systems please see Addgene’s Lentiviral Guide. While the third generation packaging... with the unconcentrated prep. For example, at Addgene we typically concentrate 100-fold meaning that ...out the lentiviral protocols and blog posts on Addgene’s website for additional resources. References ...PMCID: PMC4562269. Additional Resources on the Addgene Blog Your lentiviral FAQs answered Tips for titering...viral vector featured topic page Resources on Addgene.org Lentiviral Guide Find lentivirus plasmids Browse...
  3. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    Blog Post
    ...Resources on the Addgene Blog Learn about Synthetic Biology with BioBuilder Use Addgene Videos to Learn...School Students Explore Careers at Addgene Resources on Addgene.org Find Yeast Expression Vectors Check...these plasmids, which have been deposited with Addgene, to successfully analyze Met protein complementation...
  4. Science communication: 9 Strategies to Get Your Foot in the Door

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    Blog Post
    ...Communication Additional Resources on Addgene.org Find Job Openings at Addgene ...started:  Subscribe to Career Blog Posts from Addgene 1) Twitter – Get your voice out there Here’s one...today, Soapbox Science (hosted by Nature), and Addgene are open to guest contributions. If you decide ...BananarootsBlog.   Additional Resources on the Addgene Blog Check out Other Science Communication Posts...
  5. Plasmids 101: Optimizing Plasmid Yields

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    ...plasmid prepping is a breeze. You get your stab from Addgene, streak for single colonies, sub-culture, and prep...use a different strain. For most applications, Addgene uses and recommends DH5𝝰! This strain consistently... strain instructions from the manufacturer. On Addgene plasmid pages, you may see depositor recommendations...writing of this post. Additional Resources on the Addgene Blog Visit our Plasmids 101 Topic Page Learn How...Lab E. coli Strains Additional Resources on Addgene.org Find Info on How to Quantify Your DNA Learn ...
  6. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

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    ... Resources on Addgene.org Check out the Molecular Biology Reference Download Addgene's Plasmids 101 eBook...and Khammash's lab have created and shared with Addgene. You can find all the plasmids deposited from Di...s41589-021-00787-6 Additional resources on the Addgene blog Learn more about inducible promoters Visit...
  7. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

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    Blog Post
    ...in flies Resources on Addgene.org Find fluorescent protein plasmids at Addgene Read our Molecular Biology...recombination outcome. Find SPARC plasmids at Addgene To more precisely control the amount of labeling...s41592-018-0184-y Additional resources on the Addgene blog Learn about Gateway cloning Read about genome...
  8. CRISPR Challenges: Standardization and Homology Directed Repair

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    ...Blugene and I represented Addgene at the recent Keystone meeting on Precision Genome Editing with Programmable...desired edit, and I want to make sure that I (and Addgene) don’t forget this in the future. Here’s a list... to keep current with the latest and greatest! Addgene resources 3 Tips to Improve HDR Efficiency for ...
  9. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

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    ...When I started writing for the Addgene blog, I was focused on writing about new scientific techniques...first program I used to create graphics for the Addgene blog. However, it has a limited number of drawing...comments section!  Additional resources on the Addgene blog Read our Early Career Researcher Toolbox ...
  10. New and Upcoming Viral Vectors - December 2019

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    ...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...you pick the best AAV for your experiment. The Addgene viral service has now expanded to offer many of...pEF1a-DIO-FLPo-WPRE-hGHpA    Additional resources on the Addgene blog Read all of our viral vectors blog posts ...
  11. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

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    ...diversely applied to many different experiments. Addgene was founded in order to store, QC, curate, and ...cloning and gene expression. Just released: Addgene's Plasmids 101 (2nd Ed.) eBook And now for some ..... You can now download the newest version of Addgene's Plasmids 101 eBook! In keeping with our goal of...
  12. Hot Biosensors 2022: Year-End Roundup

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    ...new to Addgene this year. Feel the love with MTRIA sensors One of the latest additions to Addgene’s catalog...on the Addgene blog Fluorescent Proteins 101: Fluorescent Biosensors Resources on Addgene.org Fluorescent...Here at Addgene’s headquarters, the skies are getting dark and wintry with the end of year approaching...   There are hundreds of other biosensors in Addgene’s catalog — from the recently developed to the tried-and-true...
  13. An Integrin Antibody Toolkit from IPI

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    Blog Post
    ...partnered with Addgene to make our collection of integrin antibodies available through Addgene’s repository...10.1038/s41467-022-35173-w More resources from Addgene Addgene's Antibody Guide Antibodies 101: Flow Cytometry...on these cells. What’s next IPI is working with Addgene to accelerate biomedical research by distributing...
  14. Celebrating the 2024 Nobel Prize in Chemistry

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    Blog Post
    ...We’re big fans of all our depositors here at Addgene — your contributions make the repository what it...Baker Lab has deposited multiple Top7 plasmids at Addgene, along with other tools like high resolution Ras...
  15. 28 Hot Plasmid Technologies from 2015

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    ...article can be obtained through Addgene: Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885...At Addgene we're continually impressed with the amazing plasmid technologies developed by our community... Rapamycin-inducible dCas9-VP64 activator set (Addgene plasmids 62887 & 62888) Zetsche et al., Nat ...brilliant SunTag system plasmids are available through Addgene and are already very popular. Use these plasmids...pathways (Figure 2). They are all available now at Addgene. 1 Lungu et al., Chem Biol. 2012 Apr 20;19(4..., and other plasmids from this article through Addgene. Nihongaki et al., Chemistry and Biology. 2015...human genes. The plasmids are all available at Addgene ready to light up the genome of your favorite cells...
  16. Live and Let Dye: Self-Labeling Protein Tags

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    ... & Small Molecule Fluorophores. Addgene Blog. https://blog.addgene.org/better-dyeing-through-chemistry-small-molecule-fluorophores... is now the most popular self-labeling tag in Addgene’s collection. CLIP-tag: Also in 2008, Kai Johnsson...are the most popular self-labeling enzymes in Addgene’s collection, so you may be able to get a plasmid...ligand to use for your experiment, and explore Addgene's collection to find self-labeling fusion proteins...10.1021/acs.biochem.1c00258 More resources from Addgene Better Dyeing Through Chemistry Illuminating ...GCE4All: Making Genetic Code Expansion Accessible Addgene's Genetic Code Expansion Collection ...
  17. Transferable Skills: Negotiation

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    ...partner with Addgene. What are the most important issues for our partners and how can Addgene work with ...training in a variety of transferable skills. At Addgene, we've set out to fill this gap by highlighting...Richard D. Lewis Additional resources on the Addgene blog More from the transferable skills guide: ...management Managing a team Leadership Resources on Addgene.org Check out our careers page Watch our career ...
  18. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    ... and references Resources on addgene.org Addgene's CRISPR Guide Addgene's CRISPR 101 eBook CRISPR resources...nearby genes (Figure 1A-C). Find CRISPR plasmids at Addgene! Knock-in: Introducing tags or stop signals Once...resources More resources on the Addgene blog RNA Editing with Cas13 Targeting RNA with Cas13a (C2c2) Cas13d...
  19. Hot Plasmids February 2024

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    ...informed this section.  Find mStayGold plasmids at Addgene here!   Ando, R., et al. (2023). StayGold variants...primate brains with brilliance By Hridaya Patel (Addgene co-op) Researchers in the Gradinaru lab have developed...good option for essentially all applications. Addgene’s plasmid collection includes over 10,000 plasmids...both of which are also available in trial sizes. Addgene recommends these recombinant antibodies for use...
Showing: 541 - 560 of 1090 results