We narrowed to 599 results for: arl

1. Viral Vectors 101: Preparing Pooled Libraries

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   ...our more popular libraries, you might receive a similarly-sized aliquot from an amplification done by Addgene...

2. Plasmids 101: Stringent Regulation of Replication

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   ...replication of the plasmid proceeds (and terminates) similarly to the bacterial chromosome. Since replication...

3. Antibiotic Resistance: An Old Solution but a New Problem

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   ...are partially resistant. If you stop a treatment early, you may be un-wittingly selecting for less susceptible...

4. AAV Titers: Where Do They Come From And What Do They Mean?

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   ...generating a standard curve of known concentration. We regularly make and validate fresh plasmid standards. We...

5. Transferable Skills Guide: Career Planning Resources

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   ...to live in your lab, right? Wrong! While it is clearly a good idea to be diligent in your research, remember...

6. Plasmids 101: Control Plasmids

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   ...control, the internal control vector, was described earlier. Once you are sure your conditions are conducive...

7. Replacing Paper: Tips for Choosing an Electronic lab Notebook

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   ...Reproducibility Project: Cancer Biology have shown similarly disappointing rates of reproducibility. These ...

8. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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   ...signal is significantly greater than miniTurbo at earlier time points but miniTurbo exhibits less labeling...

9. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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   ...25167058. PubMed Central PMCID: PMC4412028. 5. Araya, Carlos L., and Douglas M. Fowler. "Deep mutational scanning...

10. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    ...several split vector approaches developed in the early 2000’s which allow researchers to deliver oversized...

11. Transferable Skills Guide: Conflict Resolution

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    ...identify and address these types of harmful conflict early, because time frequently does not heal these types...

12. Plasmids 101: Expressing shRNAs for RNA Interference

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    ... G., Babina, M., Bertin, N., Burroughs, A. M., Carlisle, A. J., Daub, C. O., Detmar, M., Deviatiiarov,...

13. An Integrin Antibody Toolkit from IPI

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    ...intra- and extracellular environments. Found on nearly every cell in the human body, integrins are key...

14. CRISPR 101: Homology Directed Repair

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    ...vertically at the orange arrows). If resolved dissimilarly (e.g. one junction is resolved on the crossing...

15. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    ...peptides (Santana et al., 2020) and plants viruses (barley yellow striate mosaic virus (Gao et al., 2019) ...

16. Multiple Plasmids at a Low Price: Kits from Addgene

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    ...: 2388-2393. Huang, Shijie, Xuechen Zhu, and Charles E. Melançon III. "Detection and Quantification...

17. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    ...systems. Both approaches may prove more successful early in the course of infection, when HIV-1 is limited...

18. Technologies Enabled by NanoLuc® Luciferase

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    ...orange fluorescent protein, CyOFP1. CyOFP1 was molecularly evolved from mNeptune2 through 33 mutations and...

19. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    ...al., 2023; Wu, Asokan, et al., 2006). AAV6 is particularly well-suited for transduction of lung cells via...

20. Viral Vectors 101: Optogenetic Tools

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    ...over when and where the receptor is activated.  Similarly, PTLs are special, covalently linked molecules...