We narrowed to 648 results for: ato

1. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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   ...technologies. Message or follow him on twitter @Vopinator.     References: Halpin-Healy TS, Klompe SE, Sternberg...

2. Viral Vectors 101: The Retroviral Lifecycle

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   ... Cullen, B. R. (1992). Mechanism of action of regulatory proteins encoded by complex retroviruses. Microbiological...

3. A Guide to Starting Your Own Journal Club

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   ...level of critique and detail? Who is/are the facilitator(s) of the journal club? Where will the journal...

4. CRISPR 101: Making a Knock-In Cell Line

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   ... one step further, you can inhibit cell cycle regulatory proteins responsible for the transition out of...

5. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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   ... in Parkinson’s disease. Knowing the primary investigator where the plasmid was created can narrow your...

6. MXS Chaining

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   ...construction of plasmids with many components. The creators of the MXS-chaining method have used the technique...

7. Plasmids 101: Protein tags

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   ...proteins can be purified using metal-affinity chromatography, usually using a Ni2+ column. Like other affinity...

8. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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   ...especially for young researchers. It was a really nice incubator for me. In Japan, of course, it is optimized for...

9. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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   ...optogenetics at OpenOptogenetics and find optogeneic actuators and sensors at Addgene. Cell marking/selection...

10. Your Lentiviral Plasmid FAQs Answered

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    ... the proper cell line is used; several common laboratory cell lines including 293 were immortalized with...

11. To Codon Optimize or Not: That is the Question

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    ...an organism that contains expression limiting regulatory elements or use a non-canonical code (Gustafsson...

12. Plasmids 101: Shuttle Vectors

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    ...antibiotic works. Another example is Zeocin, a DNA intercalator, which affects all kingdoms of life. Of course...

13. Twenty Years of Sharing: Expanding Accessibility

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    ...seeing our resources shared via social media, on collaborators’ websites, or as a recommendation in a Reddit...

14. Bioinformatics at Addgene

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    ...much information as possible from depositing laboratories and associated publications, but our quality...

15. New CRISPR Tools: Cas7-11 and PASTE

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    .... Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and experts in CRISPR evolution...

16. Transferable Skills Guide: Creativity

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    ...This is why perfectionists tend to also be procrastinators.  If you fall into this category, get out of...

17. Antibodies 101: Immunoprecipitation

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    ...Staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent for isolation...

18. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    ... screen did fail to identify some known ricin-regulators, but most of these genes are also essential for...

19. CRISPR-based Genome Editing Systems For Editing in E. coli

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    .... The pDonor plasmid pRE57-Ter with multiple terminators can serve to interrupt genes (or gene clusters...

20. Designing Your Chalk Talk for the Academic Job Interview

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    ...Einstein College of Medicine in the Department of Anatomy and Structural Biology, and now serves as the Director...