We narrowed to 643 results for: ato

1. CRISPR 101: Making a Knock-In Cell Line

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   Blog Post

   ... one step further, you can inhibit cell cycle regulatory proteins responsible for the transition out of...

2. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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   ... in Parkinson’s disease. Knowing the primary investigator where the plasmid was created can narrow your...

3. Plasmids 101: Protein tags

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   ...proteins can be purified using metal-affinity chromatography, usually using a Ni2+ column. Like other affinity...

4. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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   ...especially for young researchers. It was a really nice incubator for me. In Japan, of course, it is optimized for...

5. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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   ...optogenetics at OpenOptogenetics and find optogeneic actuators and sensors at Addgene. Cell marking/selection...

6. Your Lentiviral Plasmid FAQs Answered

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   ... the proper cell line is used; several common laboratory cell lines including 293 were immortalized with...

7. Bioinformatics at Addgene

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   Blog Post

   ...much information as possible from depositing laboratories and associated publications, but our quality...

8. New CRISPR Tools: Cas7-11 and PASTE

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   .... Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and experts in CRISPR evolution...

9. Transferable Skills Guide: Creativity

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   ...This is why perfectionists tend to also be procrastinators.  If you fall into this category, get out of...

10. Antibodies 101: Immunoprecipitation

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    ...Staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent for isolation...

11. CRISPR-based Genome Editing Systems For Editing in E. coli

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    .... The pDonor plasmid pRE57-Ter with multiple terminators can serve to interrupt genes (or gene clusters...

12. Plasmids 101: Shuttle Vectors

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    ...antibiotic works. Another example is Zeocin, a DNA intercalator, which affects all kingdoms of life. Of course...

13. Twenty Years of Sharing: Expanding Accessibility

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    ...seeing our resources shared via social media, on collaborators’ websites, or as a recommendation in a Reddit...

14. To Codon Optimize or Not: That is the Question

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    ...an organism that contains expression limiting regulatory elements or use a non-canonical code (Gustafsson...

15. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    ...fluorophore. The fluorescent signal can be used as an indicator to determine whether the target sequence is present...

16. Mesothelioma - Causes, Symptoms, and Treatment

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    ...several years ago – recently collaborated with Senator Barbara Boxer to introduce the Alan Reinstein Ban...

17. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    ... and is commonly used to enrich adult mouse hematopoietic stem cells. Its ligand(s) has not been identified...

18. Designing Your Chalk Talk for the Academic Job Interview

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    ...Einstein College of Medicine in the Department of Anatomy and Structural Biology, and now serves as the Director...

19. Supporting Reproducibility with a Connected ELN

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    ...researchers and PIs, science librarians and data curators, IT managers, the commercialization office, the...

20. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    ... screen did fail to identify some known ricin-regulators, but most of these genes are also essential for...