Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 952 results for: cha;

Showing: 541 - 560 of 952 results

  1. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...found in bacteria, but also exist naturally in archaea and eukaryotes such as yeast and plants. In nature...manipulate gene expression in target cells. Characteristics such as flexibility, versatility, safety, and...Produce enzymes that will make specific, controlled changes to an organism’s genome (genome engineering) Produce...

  2. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...provide new opportunities for investigating genetic mechanisms related to morphogenesis, cell fate specification...enzymatic receptor DAD2. SLs induce a conformational change of the receptor which modulates the fluorescence... a major economic impact.   Design and mechanism of action of the receptor-based SL fluorescent...

  3. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    ...widely available imaging platform: ultrasound. Challenges of in vivo imaging with existing bacterial reporters... by water-dwelling photosynthetic bacteria and archaea to regulate their buoyancy. The Shapiro lab recently...genes for in vivo imaging Following in vitro characterization, Bourdeau et al performed a proof-of-concept...

  4. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    ...Sometimes DNA sequences need to be “cleaned up” or changed in some way before you can analyze them. These ...complement sequence.  Filter DNA tool: Removes non-DNA characters, such as numbers and blank spaces, from the text... primer dimer estimation, and other primer characteristics. NEB Data-optimized Assembly Design (DAD): ...

  5. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...are distributed. Next month, we are making two changes to the policy that will make the process a bit ... the published-paper-celebration checklist as champagne or cupcakes, or maybe the lab member originally...updating our Hold for Publication policy with two changes that will help us ensure that plasmids don’t get...

  6. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ... for giving feedback Use formal and informal mechanisms for feedback (more on this below). Make sure...positive outcome if the employee sees that real change is possible in your eyes so their confidence is...feedback is a very effective way to bring about change in behaviors and habits in a nonthreatening way...

  7. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ... of freedom and diversity built into their jobs Chance to do/learn new things, diversity of responsibilities...it, we are special. Below are some special characteristics of scientists in the work place as described...identify the work style of each team member) Need challenging work, opportunities to pursue and problems to...

  8. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...single-wavelength sensors with intensities that change in response to calcium binding. There are a few...AAV5  AAV SYN flex PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation 119742  AAV5  AAV ...AAV SYN PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation Recombinases Plasmid Serotype...

  9. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...for the measurement of autophagic flux, or the changes in autophagic activity, and are a more reliable...autophagy-related microtubule-associated proteins 1A/1B light chain 3B, or LC3. LC3 is a commonly used marker for measuring...functions as an autophagy bionsensor via the same mechanism as Rosella: GFP is quenched in acidic environments...

  11. What the HEK?

    Type
    Blog Post
    ...cancer-specific research. Membrane receptors and ion channels are often exogenously expressed and studied in...proteins and its relatively low expression of native channels.  No matter what your research question, HEK-...research interests include receptor biology, ion channels, or involve human protein preparations with PTMs...

  12. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    ...independently” and “the power or opportunity to act or take charge before others do”. Candidates who have “started...including those that find events stressful. Video chatting makes it pretty easy to make 30 minute appointments...being able to just look at a paragraph and do a character count in your head.  Here are some sites that ...

  13. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ...one or two roadblocks that sparked creativity, changed the outcome of the project, or illustrates the ... and what can be left out, which can be a big challenge!  The resolution Throughout your conflict, you... and driver of your story gives your audience a chance to get curious and invested in the research itself...

  14. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...nice to have, not a need to have Must be ready to change projects frequently with little notice or choice...someone else who can do better, no grant writing Job changes can happen regularly (every 4-6 years is common... create interpersonal issues (one bad apple can change entire culture) Specific requirements for hiring...

  15. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...against invading DNA. In 2014, Swarts et al. characterized Argonaute from Thermus thermophilus (TtAgo),...expressed from a plasmid or in vitro transcribed, and changes in gRNA secondary structure can affect editing ... 3. Swarts, Daan C., et al. “Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that...

  16. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...you don’t take this past the chatting stage you will rarely make any change in your skill sets or activate...formality to mentoring Just getting together and chatting with another person about their career path is...

  18. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ... Ploidy Usually diploid, but spontaneously changes Usually haploid Usually haploid Pathology...their peroxisomes will proliferate upon a media change into methanol and peroxisomal autophagy can be ...Resources References Liu, Wei, et al., From Saccharomyces cerevisiae to human: The important gene co‑expression...

  19. Hot Plasmids - January 2023

    Type
    Blog Post
    ...Susanna Stroik The Doudna lab’s recent work characterized a new tool for RNA targeting, with a leg up ...were compared head-to-head for gene expression changes of non-target genes and RNA-seq of a nuclear (MALAT1...denaturing conditions, high temperatures, and other chaotropic conditions better than most FPs. The group also...

  20. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    ...absorb best at 280 nm and organic compounds and chaotropic salts maximally absorb at 230 nm. The A260/A280...doesn’t tell you about contaminating proteins or chaotropic salts. Capillary electrophoresis Quantifying...fragments move through a micro- or nanofluidic channel and the sample is separated by electrophoresis....
Showing: 541 - 560 of 952 results