...you labeled your gene of interest incorrectly on slides 8-16. Your notes disappear. The wrong version of... speaking is hard. Aside from the diving board charades, I doubt anyone but me remembers any of the incidents... biomedical field, with very little text on the slides and notes instead of written speeches. When you...

...is tested following each immunization until the desired level of antibodies is reached - typically within...within 2-4 months. After the animal has the desired level of antibodies in its bloodstream, the antibodies...species. Pre-adsorbed polyclonal antibodies are designed for experiments where antibodies raised in several...

... In frogs, ectopic expression of ion channels, despite having an otherwise normal genomic background, ...Subsequent cutting results in a two-headed worm, despite the fact that it’s genetically wild type  (Oveido...fluorescent voltage indicator with near-complete desensitization in continuous light. ArcLight Q239 - A GEVI...

...GFP-labeled cells. Once Tang and Cepko found a description of GFP-binding nanobodies, the project really...stable inside cells. Nanobodies binding GFP were designed in 2009. Subsequent work showed that nanobodies...green-red Cre-lox systems. T-DDOGs can also be designed with other DNA-binding domains, including the ...

...growing dialogue on the state of a system that, despite all of its successes, has many deep-rooted flaws...all bad, however - there are some signs that the tides may be turning. In 2013, the NIH announced its first...in Scientific Training (BEST) awards, which are designed to support new approaches that address the need...

...non-coding RNAs (lncRNA). lncRNAs are over 200 nucleotides in length and lack an open reading frame, so ...transcription machinery of a target gene or DNA that codes for ncRNA. CRISPRa, on the other hand, recruits ...which targets RNA instead of DNA and selectively degrades RNA molecules. This system is often used to knock...

...throughout the library preparation process and includes Picogreen quantification of various intermediate...sequences for Illumina sequencing as well as unique barcodes per sample so we can assign sequencing reads to... use a modified version of the Python program described by Joung et al. 2017, which we hope to make available...

... There are several calcium reporters specially designed for the aims and requirements of in vivo studies...populations of interest. Many of the calcium sensors described are also already packaged into viral vectors and... L, Campbell RE. Structure-and mechanism-guided design of single fluorescent protein-based biosensors....

...plasmids and pooled libraries were originally described in a peer-reviewed scientific publication. We ...scientists answering their technical support questions (besides Addgene, I know both Abcam and NEB have Ph.D.s ...believe that scientists who contact customer service deserve prompt, courteous responses. No one is likely to...

... try amplifying the viral vector with bacteria designed to minimize rearrangements such as NEB Stable ...production of virus. Alternatively, if the virus encodes a fluorescent protein, the packaging cells could...working with, there’s a lot that goes into virus design, production, and transduction. Once you’ve harvested...

... This vastly increases the possibilities when designing screens. Each serotype has its own tropism, or...experiment. Additional considerations One of the downsides to using AAV is the limited packaging capacity...Adames, A. C., Saur, D., & Rad, R. (2022). Tutorial: design and execution of CRISPR in vivo screens. Nature...

...essence, pluripotent cells can be thought of as descendants of totipotent cells. An example of pluripotent... produced Ethical concern High. Embryos are destroyed during ESC isolation. Low Chances of post-transplantation...R., M.W. Beckmann, and W. Wurfel, Non-embryo-destructive Extraction of Pluripotent Embryonic Stem Cells...

...positive and negative populations, Careful panel design In both conventional and spectral flow cytometry...indistinguishable. This tool is of particular value when designing a panel.     Figure 3: Similarity index...if markers are co-expressed in your cells when designing your panel. If you have two highly overlapping...

... also the easiest: don't use a DNA vector that encodes both Cas9 and sgRNA. What if I want to work with...one mistake can be enough. Reversal drives are designed to overwrite a previous gene drive and thereby... on popular support, which in turn depends on widespread trust in scientists to develop new technologies...

...terrific getting-started guide for BLAST, which includes a simple explanation of the different BLAST programs...matrix BLOSUM62, but you may want to check the description of the other matrices to see if another would...alternatives Depending on your sequencing result and desired analysis, BLAST may not always be your optimal ...

...Origin and purpose of MXS chaining MXS-chaining was designed to create plasmids for fluorescence imaging or... can be assembled. For example, because CpG dinucleotides are underrepresented in most vertebrate genomes...and MluI recognition sites, which contain CpG dinucleotides in their 6-mer sequences, are rarely found in...

...their production requires animal sacrifice and precludes genetic engineering of a tag or fusion with a ... with secondary nanobodies conjugated with the desired fluorophores. In figure 4B below, triple colocalization...review. If you just want to use the nanobodies described in Pleiner et al, the key steps for generating...

... can teach  5-10 year olds in a single evening. Grades K-5 cover a broad range of attention spans, skill...CRISPR work? This section was essentially background slides getting everyone on the same page in terms of the... by running it by the teachers and sharing some slides on Twitter, where I was able to get some great ...

... the data points generated by replicate values provides useful information about the accuracy of the curve...If the unknown sample’s measurement is too low, redesign the standard curve dilution series with lower ...often recommend using advanced graphing software designed to plot complex trendlines. If you don’t have ...

...Microscopy. What is Expansion Microscopy?  ExM provides a straightforward answer to the space problem....improve label density (Mikhaylova, et al. 2015).  Despite these limitations, ExM is a valuable tool, especially...Bournonville L, et al (2021) Expansion microscopy provides new insights into the cytoskeleton of malaria ...