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Viral Vectors 101: Producing Your rAAV

Type

Blog Post

... Pro tip! An optional media exchange can be performed 12–18 hours post-transfection to help remove toxic...
Pooled Library Amplifications

Type

Blog Post

...hundreds of nanograms of DNA, you will likely need to perform several individual transformations, such as splitting...
Antibodies 101: Flow Compensation

Type

Blog Post

...corrected flow plot in 2A, the populations are not perfectly straight, but the center of each population is...
Plasmids 101: Protein Expression

Type

Blog Post

...cells. Baculovirus expression systems are very powerful for high level recombinant protein expression....
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

Type

Blog Post

...PMCID: PMC4777285. Further Reading Microscopy U. Generfal Considerations when Using Fluorescent Proteins...
Addgene's Cancer Collection Pages Connect the Research Community

Type

Blog Post

...growth and proliferation, while tumor suppressors perform the opposite task, keeping cells from dividing ...
Light Sheet Fluorescence Microscopy

Type

Blog Post

...scanning microscopy . Since image acquisition is performed using cameras with electron multiplying couple-charged...
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

Type

Blog Post

...starving. I think plant biology is massively underfunded considering how important it is. I would really...
Which Fluorescent Protein Should I Use?

Type

Blog Post

...it is translated to a few hours. For example, superfolder GFP (sfGFP) and mNeonGFP can fold in <10min at...
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

Type

Blog Post

...should be free to use and used for good. It's wonderful to have Addgene as a professional public-benefit...
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

Type

Blog Post

... PCR/sequencing While CRISPR-transposons are powerful and simple to use, users should keep aware of ...
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Type

Blog Post

...of your PIPs in which the protein-interaction interface has been compromised (Kodama and Hu, 2012). Such...
SciComm: Taking Science to Elementary and Middle Schools

Type

Blog Post

...students each, and each group went on its own wonderful tangents. Some groups asked about vaccines, others...
AAVs CREATed for Gene Delivery to the CNS and PNS

Type

Blog Post

...neurons (figure 4). These capsids can serve as powerful cell-type-restricted gene vectors for neurons,...
MXS Chaining

Type

Blog Post

...of fluorescent proteins and the availability of powerful imaging technologies, the combination of multiple...
Getting to Know Expansion Microscopy

Type

Blog Post

...can achieve. This means that regardless of how powerful of an objective you have, you can’t resolve two...
CRISPR 101: Making a Knock-In Cell Line

Type

Blog Post

...increase HDR frequency by committing a break to performing HR before other factors were loaded onto the ...
Great Results Start with Great Standard Curves

Type

Blog Post

...trendline. R2 ranges from 0 to 1 with 1 being a perfect fit and 0 indicating that there is no linear relationship...
AAVs in Retinal Gene Therapy

Type

Blog Post

... the cell’s nucleus. But since nothing’s ever perfect, neither is AAV. Its main limitation is the small...
Uncovering Ancestral Bias in CRISPR Research

Type

Blog Post

... screens they analyzed were designed to have a perfect match to the reference genome – even single mismatches...

Showing: 541 - 560 of 631 results

Results per page:

Viral Vectors 101: Producing Your rAAV

Pooled Library Amplifications

Antibodies 101: Flow Compensation

Plasmids 101: Protein Expression

Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

Addgene's Cancer Collection Pages Connect the Research Community

Light Sheet Fluorescence Microscopy

Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

Which Fluorescent Protein Should I Use?

Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

SciComm: Taking Science to Elementary and Middle Schools

AAVs CREATed for Gene Delivery to the CNS and PNS

MXS Chaining

Getting to Know Expansion Microscopy

CRISPR 101: Making a Knock-In Cell Line

Great Results Start with Great Standard Curves

AAVs in Retinal Gene Therapy

Uncovering Ancestral Bias in CRISPR Research

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