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Showing: 541 - 560 of 960 results

  1. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    ...official document listing materials and pricing information that is sent from a buyer to a seller. However...your order online has some benefits. All of the information that is entered at checkout is used to generate... process, the Addgene Help Center is full of information about how to deposit plasmids, store your plasmids...

  2. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ...out an updated AAV Data Hub to share curated information about AAV usage in research applications. Scientists...what challenges remain in materials sharing, information sharing, and research reproducibility while expanding... and distributing high-quality materials and information. As a scientist, I have seen both the potential...

  3. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    ... list of citing articles under the "Resource Information" heading in the right column of the plasmid page... the "# References" link under the "Resource Information" heading, you'll be directed to a page listing...also included a feature to help save you from performing any unnecessary cloning - the list of "Associated...

  5. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ...your AAV plasmid of choice for more information on its performance.   Titers We expect titers for plasmids... and serotype. Please note that Addgene cannot perform functional or in vivo testing. We recommend you...

  6. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    ...retrograde tracing: visualizing neurons that received information from a region. In the example shown in Figure...by sharing not only reagents, but also method information. To complement these efforts, we’re beginning...through our recently launched AAV Data Hub, an open platform where researchers using AAV from our viral service...

  7. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ... extracellular surface of the GPCR causes a conformational change in the GPCR, leading to the activation...that the potency and efficacy is typically non-uniform across the transducerome for a specific receptor-ligand...Strachan RT (2020) TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome. Nat ...

  8. Fostering communication in remote collaborations

    Type
    Blog Post
    ...and improves overall communication.  Maintain informal communication In between recurring meetings, you...this casual communication reflects the type of informal communication you’d likely have with members of...equipment, or ensure that techniques are being performed the same way at different sites. I did a lab exchange...

  9. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    ...includes an antibiotic resistance marker. After transformation, plate the cells on medium containing the corresponding...selection will select for cells that have been transformed with the plasmid backbone (with or without your...scientists are finding more and more ways to creatively perform selections in plasmid cloning. Additional resources...

  10. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    ...a Biorender watermark, which is usually ok for informal settings like lab meeting presentations. You can...collections of images are easy to download in a .png file format. Images are under a Creative Commons 3.0 license...good idea to double-check the image’s license information before using it. Have a tip for using one of ...

  11. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    ...how we use next generation sequencing results to perform quality control on deposited plasmids. We’ve also...verify that our sequencing results match sequence information contributed by the depositor, published backbone...region is present in the plasmid, we might need to perform a restriction digest to confirm the size of the...

  12. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...isolate single-cell colonies, grow them up, then perform individual genotyping (using Sanger sequencing,.... You could then confirm this purification by performing a genotyping assay (such as Sanger sequencing...Cong Read Addgene's CRISPR guide for background information on CRISPR/Cas9 systems Peruse the most recent...

  13. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ...will spend 10 or more years with a small number of formal advisors learning how to be a scientist. It is ...willing to talk by phone. Don’t use email to get information if you can help it. Observe as much as you can...If lab members, neighbors or alumni give you information about the PI that concerns you, don’t just brush...

  14. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...multiple plasmids used for various platform applications. For more information on CRISPR/Cas, check out Addgene...creatively to deliver a highly specific, programmable platform well-suited for various innovative biological ...

  15. What the HEK?

    Type
    Blog Post
    ...1970s by Alex van der Eb in the Netherlands. Transformation of HEK cells with adenovirus 5 DNA was postdoc...Resources References Shaw G., et al., Preferential transformation of human neuronal cells by human adenoviruses...0995fje Tan, E., et al., HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral Vectors...

  16. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...collaboration without having to worry about proprietary information Work hours are long, but there is more flexibility...opportunities—projects end and layoffs occur, but good performers move around easily  Table 1: Taking it a Step... career with advancement in one company, good performers in for the long haul Limited movement in one...

  17. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ...these pieces aren't always appropriate for your formal scientific communications, they are fantastic fodder...Second, the introduction needs to provide enough information so that the audience can understand the conflict...scientific problem) will also help you determine what information is necessary and what can be left out, which ...

  19. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...Perhaps you have a paper, protocol, or other information that may be helpful or of interest to them. Don...you to someone who you need to speak to for informational or career purposes.  Don’t only talk about work... conference soon? Get tips here Learn about informational interviews ...
Showing: 541 - 560 of 960 results