We narrowed to 610 results for: gan
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TypeBlog Post... success of CRISPR-Cas9 across multiple model organisms (3) lies in the versatility of its delivery methods...
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Tips for Using BLAST to Verify Plasmids
TypeBlog Post..., enter the common or scientific name into the Organism box. This small piece of information can significantly... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog Post...Resistance Stability Time Saving? Works for multiple organisms? Compatible with most bacteria? No satellite colonies... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog Post...Telesnitsky, A. (2008). Pseudodiploid Genome Organization Aids Full-Length Human Immunodeficiency Virus... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post... such resources in less genetically tractable organisms lags behind. BiFC can also be used to screen for... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog Post...funding. I’ve been aware of these problems since I began my own career over 20 years ago, and I’m sure they... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog Post...of COVID-19 effect on plasmid requests Before we began any sophisticated analyses, we first visualized ... -
Droplet Digital PCR for AAV Quantitation
TypeBlog Post...Since Addgene began producing AAV vector preparations, we have used quantitative PCR to evaluate the ... -
AAVs in Retinal Gene Therapy
TypeBlog Post... as an ideal target. It is an easily accessible organ that can be monitored non-invasively over time. ... -
MXS Chaining
TypeBlog Post...however, be appropriate for cloning genes from organisms with high CpG dinucleotide representation. The... -
Tips for arabidopsis transformation
TypeBlog Post...Stanford University. Arabidopsis is a fantastic model organism for many reasons, not the least of which is ease... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post...just starting a lab, or are looking for a way to organize samples within your lab, we highly recommend using... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog Post... Kendall Morgan... -
Negotiating Work and Life: How to Find the Joy
TypeBlog Post...temple, my kids’ school, and with professional organizations supporting women in science and career development... -
Antibodies 101: Reading a Flow Plot
TypeBlog Post...Vegas, A., Ma, M., Sahay, G., Chiu, A., Bader, A., Langan, E., Siebert, S., Li, J., Greiner, D., & Anderson... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog Post...drosophila, worms and mammals (Browse plasmids by model organism, here). More information on the various delivery... -
Bioinformatics at Addgene
TypeBlog Post... collection of scripts evolved into a single, organized pipeline and accelerated the processing of our... -
Viral Vectors 101: Pseudotyping
TypeBlog Post...more safely. Recently, many SARS-CoV-2 researchers began pseudotyping the SARS-CoV-2 spike protein into a... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog Post...on the escape mechanism of genome editing in microorganisms which could be applied to optimization of genome... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog Post...NGG VQR variant D1135V, R1335Q and T1337R NGAN or NGNG EQR variant D1135E, R1335Q and T1337R...