Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 850 results for: met

Showing: 541 - 560 of 850 results

  2. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...sense ssRNA, or negative sense ssRNA) and varying methods of replication. In fact, an entire classification...single particle creates many copies of itself, sometimes up to millions of new copies, which are released...

  3. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...Isotype matters when constraining using the indirect method since a secondary antibody reactive to a certain... not be used for multiplexed indirect staining methods since the secondary antibody used will react to...

  5. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...surprisingly more rigorous in industrial settings Tight (sometimes impossible) timelines—others are usually waiting...people) -> How can you make a decision about something like this without sufficient data? Starting to...

  6. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...limited expression levels of XFPs in the original method, we used the Tetracycline trans-activator system...Improved tools for the Brainbow toolbox." Nature methods 10.6 (2013): 540. PubMed PMID: 23817127. PubMed...

  7. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...Topoisomerase based cloning (TOPO cloning) is a DNA cloning method that does not use restriction enzymes or ligase...to decrease error rate, please use one of these methods to ensure your insert retains the 3' A overhang...

  9. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...unwavering devotion to seeing your ideas become something tangible.  When you think of entrepreneurship ...heavy price tag, especially if you're working on something other than software.  If you are a graduate student...

  10. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...description of the lab’s iTOP protein-only transduction method and its potential applications to CRISPR/Cas9 genome...osmocytosis and propanebetaine) to describe this method of protein transduction. Later studies showed that...

  11. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...in college that I really fell in love with it. Something about being able to actually hold all those seemingly...educational uses. We believe that biology education is something that should be available to everyone, and hope...

  13. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...Staining. https://serc.carleton.edu/microbelife/research_methods/microscopy/gramstain.html Smith, A. & Hussey...medicine and more successful experiments. It’s something that all scientists should understand when stepping...

  14. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...visualizing glutamate neurotransmission.” Nature Methods (2013) PubMed PMID: 23314171. PubMed Central PMCID...variants of the glutamate sensor iGluSnFR.” Nature Methods (2018) PubMed PMID: 30377363 Aggarwal, Abhi, et...

  15. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...is because the host lab or institute provides something that you can’t obtain at your current institute...notes, and ask questions if you don’t understand something. Offer to help out wherever you can and don’t ...

  18. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...vesicles or microvesicles (approx. 40 - 150 nm diameter) that are secreted from the cellular membrane ...Overexpression of the Tetraspanin CD9." Molecular Therapy-Methods & Clinical Development 9 (2018): 278-287. PubMed...

  20. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...Ribosome profiling Ribosome profiling, a biochemical method developed in 2009, takes a “snapshot” of all of...UTR were translationally silent. Both of these methods increase our understanding of translation dynamics...
Showing: 541 - 560 of 850 results