...proteins. lon protease: Strains where this is completely deleted (designated lon or Δlon) similary reduce...variety of sources, so fear not—there are a few simple troubleshooting measures that can help get you ...will not express in an IPTG induction strain for example, nor will a p15 plasmid be compatible with a pLys...better at 30°C or room-temperature, which is accomplished by growing your culture to the desired density...

...are essential cell surface receptors involved in complex signaling pathways and the linkage of intra- and...development. Integrin structure and conformational complexity There are 24 known mammalian integrins, all heterodimers...demonstrated that anti-integrin αVβ5.9 “essentially completely blocks all adhesion,” inhibiting the function...Leucine-Valine-Aspartate-Proline (LVDP) motif. The Ha and Springer teams employed IPI synthetic antigen-binding fragments (Fabs)...

...scFv sequences have traditionally been cloned by amplifying the VLand VH antibody sequences of hybridomas...applications of scFvs: The SunTag system uses an scFv to amplify the fluorescence intensity of a tagged protein.....0000000000000145  Gąciarz A, Ruddock LW (2017) Complementarity determining regions and frameworks contribute... about the SunTag system which uses an scFv to amplify the fluorescence intensity of a tagged protein....

...protein is cleaved by proteases. Nowadays, with ample structural information available, it’s clear that... better preserves the function of the POI. For example, the APT1-mVenus fusion localizes at the Golgi,... interact with other proteins or biomolecules. Examples of the successful insertion of a fluorescent protein...interactions) can be challenging. If a fusion can complement a knock-down or knock-out of the corresponding...

...important microalgae: intrinsic suicide switch implementation in cyanobacterium Synechocystis sp. PCC 6803...The Gomis-Ruth lab designed the pCri system to simplify the protein purification process by making it ...directly engages the ribosome for translational complex assembly. PhD student Dejan Gagoski used SITS ...Illumina-M13F bridging primer. These bridging primers are amplified and used to generate Illumina sequencing libraries...

...build suspense. Notice the simplicity of their slides and how the slides complement the spoken message. Learn...get people to remember you). What better way to accomplish all of these things naturally than to give a ...cause and effect. Stories can and should be very simple. The goal is to get the listeners to be thinking...

...Gene knockout with CRISPR technology is usually accomplished by Cas9-mediated dsDNA breaks: following a cut... into DNA. This also requires an exogenous DNA template. HDR, however, is a very low-efficiency process... is >30nt from the proximal ends of the repair template (Yang et al., 2013). This means that, for gene...but can often require difficult decisions. For example, would it be better to use a less-active gRNA that...

...Using AAV vectors in complex biological systems can be tricky at best, and downright infuriating at worst...what works great in one system or cell type can completely fail in a different system (Issa et al., 2023...postsynaptic transmission (Oh et al., 2020). For example, AAV1 and AAV9 tend to spread farther from the ...Off-target expression can still occur (Fig. 3) - for example, some neurons can transiently express TH - so you...

...genetic code of living organisms and its societal implications even before the discovery of genes themselves...repaired by homologous recombination with a repair template.  Each zinc finger recognizes a nucleotide triplet... and reverse transcriptase. Using an elongated template + guide RNA called pegRNA (prime editing RNA),...us to always consider the ethics and broader implications of our research.  Want the Genome Editing Trading...

...Bernstein and Boyden, 2011; Mei and Zhang, 2012). For example, blue light activates channelrhodopsins that, in...photo-stimulated through fiberoptic cannulas directly implanted in the injection site and connected to a laser...Abdallah et al., 2018; Deubner et al., 2019). For example, Taylor and colleagues used rAAV FLEx-based vectors... Han, 2016). Not so long ago, however, it was completely unclear which subpopulation of DA neurons could...

...April because devaluing science has alarming implications for our public policies—many of which impact..., challenged us. I worried the march would be a simple, unquestioning celebration of science, at a time...funding, and teaching experience that it takes to complete your resume, without ever thinking that research...research scientist would be a career that could simply cease to exist. But then November hit and panic ensued...

...Fluorescent proteins (FPs) offer scientists a simple yet powerful way to tag cellular proteins and investigate...another (e.g. green to red, or cyan to green). For example, mEosFP (named after the goddess of dawn in Greek...trafficking and dynamics (fission, fusion, etc). For example, by engineering PA-GFP tagged to a mitochondrial...variants are more monomeric than mEos2 (mEos4b is completely monomeric) and display bright and photostable...

...,000 plasmids, kits, and viral service items we employ dozens of pieces of energy-intensive lab equipment...certainly not the only sustainability effort we’ve implemented at Addgene. The kitchen and office areas provide... new programs, we're always striving to set an example of environmental stewardship.  Additional resources...

...in the biological sciences and techniques that simplify and expedite research. We recognize, however, ...Blog is a great way to get your name out there! Completed blog posts are also great to include on CVs and... blog! To sign up to write for the Addgene blog simply email us. We can’t guarantee that all those who...

...be used to cut the DNA sequence. With a repair template, CRISPR can be used to introduce a functional ...single base changes in the DNA sequence without completely cutting the DNA. RNA Editors: CRISPR nucleases...CRISPR nucleases that can’t cut DNA (dCas9, for example) that are used to increase or decrease expression...

.... To start the AAV packaged on request process, simply navigate to your plasmid of interest. If it’s eligible...received, we’ll review for technical and legal compliance, and then send you an email with a link to order...There are no quotes or special processes involved; simply request your plasmid of interest in your desired...

...antibodies, for example through the use of less toxic adjuvants; 2) generating complex antibody chips ... assessment of many protein targets in a single sample; 3) producing recombinant, partially humanized ...

...cultural shifts that can have bigger impacts. For example, if you joined an office where everyone used a ...long-term goals to stay motivated and to feel accomplished. In addition to doing some of the quick things... about this, and even has a list of biological samples that are stored long term at -70 °C. My Green Lab...

...Addgene, we try to make the process as smooth and simple as possible. We are the Customer Support Specialists... Placing an order on Addgene’s website is quite simple, even if you’re using a PO number. Follow these...time, your organization will have net 30 days to complete payment for your order.  Still have questions?...

...visualization in vivo using ultrasound (for an example see the figure below). In addition to low costs...Approaches to Presenting the Microbial World’ showcased complex connections and ideas in various visualized approaches...gifs created by the Amoeba Sisters. Here’s one example of how they “bridge the jargon gap using gifs”:...