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  4. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...need that free hydroxyl group! You may want to include extra extension time after the last cycle of PCR...

  9. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...can find FLAG antibodies on Addgene’s catalog including a mouse monoclonal (M2) as well as a chimera (...

  13. Plasmid Cloning by PCR

    Type
    Blog Post
    ...similarly, taking the final 18 bases of the ORF, including the stop codon (5'-TGGCATATCTCGAAGTACTGA-3'), ...
Showing: 541 - 560 of 758 results