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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,052 results for: sta

Showing: 541 - 560 of 1052 results

  1. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...but many throughout the world are affected by devastating diseases that affect far fewer people and therefore...provide hope to patients suffering from these devastating afflictions. Elia Di Schiavi - Spinal muscular...expression in specific cells. With a more concrete understanding of the molecular biology behind SMN1 and its...screens. He plans to work with a partner at IRCCS Besta in Milan to test small molecules targeting SMN1 ...Nabavi is a Postdoctoral Fellow at the Vancouver Prostate Center and is affiliated with the University of...

  2. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ... process will look slightly different from our standard process. We’ll walk you through a brief overview...be able to request Packaged on Request service starting in January 2025.  What will the ordering process...flexibility; while ensuring we can maintain high standards for both our depositors and requestors.  Requesting...specialist will reach out to you during the request stage, before you order.  Timeline Once your order is ...place a request and order if approved.  I want to stay updated!  We want to keep you updated! Subscribe...

  3. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...many of the speakers.  As Dr. Polka said as she started things off, “We are all capable of contributing....  His list is dire:  The current system is unsustainable, hypercompetition is corroding values, we are... away from PI driven basic research, there is resistance to change, public support is weak, government...pretty much said it all.  She cited the oft quoted statistic that only 15% of current postdocs will be academic...drastic change in the way we train scientists to start addressing this problem.  Finally, one point made...

  4. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...post was contributed by Jordan Ward who is an assistant professor at the University of California, Santa...elegans relied on selective markers such as drug resistance (Chen et al., 2013), fluorescence (GFP; Tzur ...unedited animals struck me as a “needle in the haystack” type of problem and led to me to explore alternate...lethal mutation should remove much of the unedited “haystack”, facilitating recovery of edited animals. ...allowing novice researchers to quickly and easily start genome editing (Schwartz et al., 2018). To see more...Thank you to our guest blogger! Jordan Ward is an assistant professor at the University of California, Santa...

  5. Antibodies 101: Validation

    Type
    Blog Post
    ...come together over the past few years to propose standards and recommendations for improving antibody use... is all it will bind to - particularly when you start using complex samples such as whole cell lysate ...be aware of any possible alternative translation start sites or alternative splicing that could lead to...bound to the target protein, so it’s important to understand if protein:protein interactions could be a factor...Addgene.org Find plasmids encoding antibodies at Addgene Stay tuned for ready-to-use antibodies from Addgene Designing...Bradbury A, Plückthun A (2015) Reproducibility: Standardize antibodies used in research. Nature 518:27–9....

  6. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ... longer than necessary before starting your experiment, or if you start an experiment before adequate ... or frt-STOP-frt (FSF) sequence prior to an ATG start codon to prevent transcription in cells where the...Therefore, the first step in any AAV experiment is to understand which serotype best transduces your cells of ...the serotype, except now you will already have a starting “optimal” injection volume and titer to begin ...vectors in your experiments requires a full understanding of vector performance in your system. It is ...

  7. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ... optimization to increase the specificity and stability. Optimizing antibodies for better pharmacokinetics...regions can be altered for specific functions, the constant regions can be altered for different isotypes,...Fab: fragment antigen binding; Fc: fragment crystallizable; IgG: immunoglobin G; ScFv: single-chain fragment...cycle, your best-performing mutant is usually the starting point for the next cycle. Figure 2: General...Instead of leaving sequence generation to chance, you start with a known or predicted structure of your antibody-antigen... more, the best mutant can then be used as the starting point in the next round of mutant library generation...

  8. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ...The biomedical field is often concerned with understanding the cause of diseases and how to treat those...diseases. The “cause of disease” often requires understanding the disease genetics and the “treatment” usually...time and money gets put into them in those later stages. Target identification Not all diseases are fully...that target and validate the hit compound. The standard is to test the drug on models which are genetically...are selected.      CRISPR aids in multiple stages of the drug discovery pipeline.   CRISPR ...

  9. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    ...this experiment? Can you follow and understand the protocol from start to finish?’" says Caitlin. SME’s...well.’” Stay tuned for the next feature in this series. We’ll be interviewing Susan Keown, a staff writer...!'" Working as a subject matter expert Caitlin started out as a subject matter expert (SME) at eScience...

  10. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ...Feedback is help and it should result in authentic assistance for the recipient to become more effective and...successful manager. Great bosses tell people where they stand clearly and routinely. They are clear with each ...questions after a task or period of work. Using this standard format not only enables you to discuss a person's...looking for a new position. One of the most common mistakes I see is managers who hint at or use indirect ...Steven Pinker. As a manager you must be able to state your observations truthfully and in clear terms....

  11. Advice for Choosing a Research Project

    Type
    Blog Post
    ...community. In the long term, the research project establishes your professional brand. It offers colleagues... as a stepping stone into the next professional stage of your career by connecting you with people of ...plan? Have you read the relevant literature to understand where the field is today? Have you talked to ...to use to reach these milestones? Can you get assistance from people who have the required expertise to...Choosing a Research Project Advice for Scientists Starting a Lab    ...

  12. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    ...use, and likes to start at the beginning, shepherding a new product from the idea stage through all of its... days or even weeks. Primer design was done painstakingly by hand. Records of plasmid construction were... plasmids contain features such as antibiotic resistance markers and replication origins, but there is...efforts, such an algorithm has limitations. For instance, it could miss a common feature if the sequence...

  13. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    ...the plasmid here yet? A quick look at your order status on addgene.org identifies the hold-up. Your Material...Golden Stopwatch Award recognizes those TTOs with outstanding material transfer agreement (MTA) turnaround ...request MTAs and deposit agreements to basically instantaneous in many cases.  Suggest that they get an Addgene...possible new awardees, and we’re happy to share stats and strategies with tech transfer offices. Drop ...Sloan-Kettering Cancer Center  Monash University  Montana State University  National Institute of Diabetes & Digestive...

  14. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    ...and all the shortcomings and mistakes pointed out. Therefore, I try to stay away from the loaded and judgemental...ensuring that published work meets the accepted standards of reporting in the discipline and reduces the...underscore the sentences that I found hard to understand or individual words that are not appropriate ...whoever cares to listen. In March 2020 she will start her own research group at Boyce Tompson Institute...

  15. RUBY-Red Siliques

    Type
    Blog Post
    ...antibiotic/herbicide resistance and fluorescent proteins. Selection using resistance genes usually involves... editing, transformation may be identified by resistance to an antibiotic. Successful editing of transformants...requiring the destruction of plants or tissue for GUS staining or are not suited to studying large samples. RUBY...may contribute to the emergence of antibiotic resistant disease. (RUBY-red: You can’t beet it!) About...

  16. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ... and communication. A great approach to fully understand and acquire competitive skill sets in a myriad...with a strong impact, or a journalist does not understand the high-level science discussed in an interview...your project may not advance beyond its initial stages. Help you build a network outside of the academic... incorporating EAs into your routine.  Getting started: Planning your career path and identifying needed...Science writing competitions (e.g. Access to Understanding)   Writing Communication Publicity ...

  17. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...indicating translational repression. During later stages, single RFP-labeled osk and Oskar protein were ...mRNA and keeps it in a single field of view for constant tracking. You can then directly monitor multiple...used harringtonine, a translation inhibitor that stalls ribosomes after initiation, to calculate a ribosome...silent. Both of these methods increase our understanding of translation dynamics, but they come with ...translation in various cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide...

  18. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...out to start the conversation. Many researchers are open to sharing knowledge and establishing collaborations...perform research there temporarily. The length of stay could be from a month to a year. The researcher ...take good notes, and ask questions if you don’t understand something. Offer to help out wherever you can...a really good time! If it sounds right for you, start thinking outside of your current research box, send...

  19. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...profound, and this is a rare instance in which "profound" seems like an understatement. Multiple speakers from...be of secondary importance (or tertiary, or non-existant). On the very first night of the iPSC symposium... Christine Mummery, Lorenz Studer, and Deepak Srivastava--addressed a battery of questions regarding topics...implant human kidney progenitors into a pig. In stark contrast to recent genome engineering technologies...

  20. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...individual fluorescent protein (brightness, photostability, and so on; see the previous blog post for ...CFP and GFP (ex 488 nm / em 507 nm) show some crosstalk between the two fluorescent proteins. If you must... microscope. Ideally, you would like to have substantial overlap between the excitation and emission filters...most recently published ones), they can be a good starting point. In many cases it is sufficient to use a...screenshot from the Chroma Spectra Viewer comparing a standard Cy3 or Rhodamine filter set (Chroma #49004) to...
Showing: 541 - 560 of 1052 results