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We narrowed to 958 results for: tes

Showing: 541 - 560 of 958 results

  1. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...be used to express proteins. Viral expression cassettes typically contain a promoter (inducible or constitutive...lineage, and so much more! Libraries allow you to test thousands of elements simultaneously in your system...dose. Vaccine development strategies. Image courtesy of Gorry et. al, 2007.   Clinical applications...

  2. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...can rapidly and efficiently evaluate Cas9 cutting sites and activity (Knudsen et al., 2018).  To create ...lab compared editing at three different genomic sites in the AAVS1 locus using GEmCherry2. In this experiment...again that sgRNA structure and genomic recognition sites play a large role in CRISPR-Cas9 efficiency. They...

  3. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ... start, the team inserted AsLOV2 into conserved sites in all eight surface-exposed loops of an mCherry...SOS), a guanine nucleotide exchange factor that activates Ras. They expressed this OptoNB-SOS fusion protein...membrane-localized mCherry. With darkness, SOS binds Ras and initiates the extracellular signaling-regulated kinase (...

  5. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...below). Plating the transformation on ampicillin plates containing X-gal allows you to identify cells that...that when a portion of the gene is deleted, it creates a non-functional 𝛽-galactosidase. Complementing...this deleted portion, for example on a plasmid, creates a functional 𝛽-galactosidase. Blue-white screens...

  6. Hot Plasmids - May 2022

    Type
    Blog Post
    ... both on and off target. Across all loci tested, error rates overall ranged from 0.3% to 10%, in comparison...Importantly, this is true in both mice and nonhuman primates (for AAV.CAP-B10, for example).  Given that the...

  7. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ... trans activity. AsCas12a, on the other hand, generates staggered DNA nicks at its target in cis and cleaves... used by Jinek et al. 2012, or browsing for the latest AsCas12a plasmids, Addgene and CasPEDIA have got...from CasPEDIA.   How can I use it? CasPEDIA operates like many search engines, featuring both a general...

  8. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...calculated (details in [2]) and can be used to quantify rates of mutation in a bulk or single cell-derived genomic...instructions on how to design such assays, in addition to testing their performance (2). Remember that although digital...transfection of a novel TAL effector nuclease (TALEN) facilitates efficient knockout of HIV co-receptor CCR5. Nucleic...

  9. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...together. I also prefer the number system to using dates as labels, since I often work on multiple projects...sure that the original code remains in tact! Take notes Comment in your scripts: Comments on what a given...who are still learning the basics. However, these notes take up a lot of space and don’t tell you why you...

  10. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...infancy. A long timeline from transformation to testing and a lack of well-characterized genetic tools ...excited to see how this exchange of genetic tools facilitates plant science. Challenge #3: Intellectual property...in 2004, she accepted a commission in the United States Air Force. She left active duty to pursue a degree...

  12. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...caveolin 1, also known as CAV 1. Usually, caveolin 1 promotes the cell life cycle progression and enhances tumor... TAK 1, an enzyme that controls apoptosis and regulates transcription. Without TKA1, cells proliferate... 2004). In fact, Sanford H. Barsky and his team tested the influence myoepithelial cells had on the conversion...

  13. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...with the desired resolution of prime editing intermediates. Building upon the PE2 and PE3 systems of their...introduce epitope tags and RNA transcription start sites. The lab further demonstrated that PRIME-del can...RT), which induces two double-strand breaks and deletes the intervening sequence. As with other prime editing...

  14. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...keep track of how you met the connection, as these notes are saved in the messaging stream. Unfortunately...LinkedIn is not the platform for politics, family updates, or click bait of any kind. For more on inappropriate...However, do comment on other people’s news and updates. Share your own good ideas, events and favorite...

  15. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...antibiotic in your plate might not work. Check the plates! Streak on them an E. coli  strain known to be ...growing fine, it is probably time to prepare new plates with a fresh stock of antibiotic. Many small colonies... different temperature (30°C) for the growth on plates and in liquid culture. If you don’t get any positive...

  16. Summer SciComm: Preprints

    Type
    Blog Post
    ...Photo courtesy of Henry Mühlpfordt, under a CC BY-SA license. Many scientists and advocates have argued...manuscript is drafted. Preprints were one of the key routes for researchers to share information about the ...

  17. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...large number of targets, and can require specific conjugates. And, of course, once you have them, you’ll need... along with a higher likelihood of aggregation (Bates, 2019). They are small enough to be produced in ...delivery systems. Elsevier Science & Technology Books. Bates A, Power CA. David vs. Goliath: The Structure, Function...

  18. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...options. With limited time and budget you cannot test them all, so how do you know which ones to try?  ...immune complexes are smaller than a primary:secondaries:conjugates complex. If you are trying to visualize....  The 5 pillars include genetic strategies that test expression in knock-out or knock-down down tissues...

  19. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ... oxygen concentration may affect photobleaching rates (Shaner et al, 2008). This brings us to the key ...application (Goedhart et al, 2012). The third issue relates to the environment of the fluorescent protein. ...The issues with quantification of photobleaching rates can be summarized as follows: Instead of determining...

  20. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ... cross-talk with the other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric...individual colors to 6. A gRNA with all three hairpins generates white light. All 6 gRNAs can be expressed in a...separate vector. Figure 2: CRISPRainbow generates 6 unique colors and a white composite. gRNAs with...
Showing: 541 - 560 of 958 results