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We narrowed to 962 results for: tes

Showing: 541 - 560 of 962 results

  1. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...infancy. A long timeline from transformation to testing and a lack of well-characterized genetic tools ...excited to see how this exchange of genetic tools facilitates plant science. Challenge #3: Intellectual property...in 2004, she accepted a commission in the United States Air Force. She left active duty to pursue a degree...

  2. Antibody Guide

    Type
    Guide
    ...response has developed, the antibodies are collected, tested against the antigen, packaged, and sold. This is...immortalized B cells). The genetic reorganization that creates variety in the antibody allowing for their extreme...class therefore has a valency, or number of binding sites available per antibody unit, which is typically ...multiplied by two, as each antibody has two binding sites. Isotype Description Configuration and Valency Subclasses...Monomer with a valency of 2 IgE Protects against parasites and is responsible for driving allergic reactions...in the lab. Check for and record any expiration dates. Buffers Buffers are a concern for conjugating and...reaction in the presence of several different substrates. The luminescence produced by the reaction can...

  3. Plan Your Experiment

    Type
    Guide
    ...additional sites throughout the genome. These sites are called off-targets. Be aware of off-target sites when...efficiency. So while testing multiple gRNAs is a good idea for most CRISPR experiments, testing multiple pegRNA...efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing. Scientific...that there could be downstream translation start sites that could allow for expression of a truncated protein...PAM sequences or Cas9 enzymes with flexible PAM sites . Base Editing : Target the site of your desired...complement to your target sequence with no homologous sites elsewhere in the genome. In reality, a given gRNA...genetic cargo (in this case, CRISPR components) integrates into the genome. Lentiviral vectors are a good...

  4. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ... oxygen concentration may affect photobleaching rates (Shaner et al, 2008). This brings us to the key ...application (Goedhart et al, 2012). The third issue relates to the environment of the fluorescent protein. ...The issues with quantification of photobleaching rates can be summarized as follows: Instead of determining...

  5. Summer SciComm: Preprints

    Type
    Blog Post
    ...Photo courtesy of Henry Mühlpfordt, under a CC BY-SA license. Many scientists and advocates have argued...manuscript is drafted. Preprints were one of the key routes for researchers to share information about the ...

  6. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...large number of targets, and can require specific conjugates. And, of course, once you have them, you’ll need... along with a higher likelihood of aggregation (Bates, 2019). They are small enough to be produced in ...delivery systems. Elsevier Science & Technology Books. Bates A, Power CA. David vs. Goliath: The Structure, Function...

  7. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...options. With limited time and budget you cannot test them all, so how do you know which ones to try?  ...immune complexes are smaller than a primary:secondaries:conjugates complex. If you are trying to visualize....  The 5 pillars include genetic strategies that test expression in knock-out or knock-down down tissues...

  8. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...with the desired resolution of prime editing intermediates. Building upon the PE2 and PE3 systems of their...introduce epitope tags and RNA transcription start sites. The lab further demonstrated that PRIME-del can...RT), which induces two double-strand breaks and deletes the intervening sequence. As with other prime editing...

  9. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    ...the idea of general interfaces and the freedom to test out lots of combinations, to end up finding something... which are useful for connecting proteins in the test tube or in cells. There are also SNAP-tag and HaloTag...template VirD2 HUH-tag For creating DNA-protein conjugates rHUH HUH-tag (RNA) Covalent protein tag for...

  10. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ... cross-talk with the other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric...individual colors to 6. A gRNA with all three hairpins generates white light. All 6 gRNAs can be expressed in a...separate vector. Figure 2: CRISPRainbow generates 6 unique colors and a white composite. gRNAs with...

  11. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ...a new legal minimum of $47,476 per year, under updates to the Fair Labor Standards Act (FLSA). This was...22nd a preliminary injunction against the FLSA updates was granted nationwide. Again, we at Future of ...getting paid, which we will be analyzing for future updates. But suffice it to say, salaries are showing surprisingly...

  12. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...keep track of how you met the connection, as these notes are saved in the messaging stream. Unfortunately...LinkedIn is not the platform for politics, family updates, or click bait of any kind. For more on inappropriate...However, do comment on other people’s news and updates. Share your own good ideas, events and favorite...

  13. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...antibiotic in your plate might not work. Check the plates! Streak on them an E. coli  strain known to be ...growing fine, it is probably time to prepare new plates with a fresh stock of antibiotic. Many small colonies... different temperature (30°C) for the growth on plates and in liquid culture. If you don’t get any positive...

  15. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...caveolin 1, also known as CAV 1. Usually, caveolin 1 promotes the cell life cycle progression and enhances tumor... TAK 1, an enzyme that controls apoptosis and regulates transcription. Without TKA1, cells proliferate... 2004). In fact, Sanford H. Barsky and his team tested the influence myoepithelial cells had on the conversion...

  16. Working with Nuclear Receptors

    Type
    Blog Post
    ...and disease states, their circadian regulation, as well as in important physiological states such as adipogenesis...therapeutic indices, including hypertension, cancer, diabetes, cardiovascular disease, cholesterol gallstone...

  17. Lentiviral Vector Guide

    Type
    Guide
    ...incompetent by using four plasmids instead of three and eliminates the requirement for Tat. Always includes deletion... transfer plasmids may have limited restriction sites or may only be compatible with certain cloning methods...target species. Although chromatin availability facilitates integration, it does not explain the lentiviral...more accurately predict and understand integration sites have led to the development of multiple genome-wide...al. (2020) for examples). Pseudotyping Tropism dictates which types of host cells the lentiviral vector...glycoprotein — transduce airway epithelium and cardiomyocytes in utero Rabies glycoprotein — transduce neural...for genome-wide mapping of lentiviral integration sites. Molecular Therapy — Methods & Clinical Development...

  18. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ... high complexity Golden Gate Assemblies. Image courtesy of New England Biolabs.   DAD Tools The three...input a sequence, SplitSet finds optimal fusion sites to split it into multiple fragments. You can define...sequences or set a narrow search window for a site—or sites—that must be used. Each of the tools works with ...

  19. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ... experiments is cutting efficiency so that the greatest effect possible will be achieved. On the other... an NG PAM.   Cas PAM sequence  Additional notes SpCas9 NGG - Cas12a TTTV Great for AT-rich...coverage in the human genome. SpRY Cas9 has PAM sites covering the majority of the genome! Want to...

  20. Hot Plasmids February 2024

    Type
    Blog Post
    ...co-incubate it with an “assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral... in rodents have not been widely successful in primates. The authors used a multispecies approach to generate...endothelial-specific tropism in rodents and broad tropism in primates. Nature Communications, 14(1), 3345. https://doi.org...
Showing: 541 - 560 of 962 results