We narrowed to 819 results for: ust

...affect binding the lysis buffer may need to be adjusted depending on the sample. It is important to remember...make this approach appealing, but the disadvantages must be carefully considered before using. For example...

...are 500–800 bp smaller than previous editors, but just as effective and with no additional off-target edits...on-target delivery of genetic cargo  By Alyssa NeuhausTo improve delivery and reduce off-target effects...

...control by Addgene before being distributed to customers. Details about our production protocols, titering...Addgenies and most recently updated by Ina Ersing in August 2023. Resources and references Resources at the...

...coding sequence of interest (CDS1, top). Overhangs must match the Level 0 acceptor (bottom) for CDS1 parts...the learning and planning, and your MoClo kit has just arrived! MoClo kits from Addgene will most likely...

...today (Fagone et al., 2012). PCR-based methods are robust, easy, fast, and convenient. However, these methods... starting material in the PCR because the sample must fall within the linear region of the standard curve...

...drug resistance status, it is a very common, well-trusted method that is frequently used in the clinical ...final report and recommendations." London: Wellcome Trust & HM Government (2016). 2. CDC Information Pages...

...plasmids used in the validation assay (above) and it is just as effective (Mashiko et al., 2014). The plasmid...needed when dealing with RNA (e.g. face mask, and a dust free and specially cleaned work bench). Once the...

...plants, most bacteria and some fungi, thus mammals must obtain the vitamin through external sources. Humans...biotin and avidin, macromolecules of interest first must be biotinylated. Scientists have developed several...

... difficult to predict which one will work as illustrated in figure 3 (Mastop et al., 2018). Unbiased, ...Jakobus, et al. "A New generation of FRET sensors for robust measurement of Gαi1, Gαi2 and Gαi3 activation kinetics...

...been following Addgene on #souptwitter or if you just enjoy a warm meal on a winter day, you should be...(Alam et al., 2017). Yellow fluorescent aptamers Just as vegetables come in a variety of colors, so do...

...By Justin Ng and Alyssa Cecchetelli Science is a constantly evolving and demanding field, requiring a...experiments! References: Blount, Z. D. (2015). The unexhausted potential of E. coli. ELife, 4. https://doi.org...

...video from iBiology). IHC and ICC are essentially just more general terms that can also refer to assays...fluorophores. It is common for labs to keep a few trusted secondary antibodies on hand to mix and match with...

...complementary to their target sequence. However, you can’t just use the mature sequence in a plasmid and call it...common issue with many gene silencing methods and just needs to be taken into account when designing experiments...

...candidate in Neuroscience at Harvard University. Just as computers, cell phones, and cars become more ...throughout the amplification process, rather than just at the end as in standard PCR. This allows the researcher...

...sites (like, indeed.com, or salary.com). Search industry and academic job titles in the regions where the...to proceed with a used or new item. To further illustrate this point, if your research is dependent on ...

... as tightly packed and interwoven as the brain, robust single-cell labeling is necessary to distinguish...anemone mKate2.) They then successfully generated custom antibodies to each of these proteins and confirmed...

...genetic tools that consist of two parts, both of which must be present for a gene of interest to be expressed...Liqun Luo’s lab developed the Q system from a gene cluster in the fungus Neurospora crassa. The QUAS promoter...

...metastatic capacity of cancer cells. This is certainly just the beginning for MitoTRACER, as it is a diverse...advantages. While most red GECIs have been derived from just a few parent fluorescent proteins, PinkyCaMP is ...

...special: Strong expression: A CMV promoter drives robust gene expression in mammalian cells. Flexible tagging... Antibody Data Hub. Perhaps these antibodies are just the tools you need to push the field forward! Browse...

...it off, allowing the cell to be excited over a sustained period of time (Gong et al., 2020). Light-gated...Bendifallah, I., Zhou, F., Zeitzschel, N., Ahlbeck, J., Augustin, S., Sauter, K., Papagiakoumou, E., Gottschalk...