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We narrowed to 938 results for: Kin

Showing: 281 - 290 of 938 results

  1. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...Mortensen, a PhD candidate at Northeastern University. Working with plants doesn’t always have to be a time-consuming...recently overcome this problem in C. roseus by working with young seedlings and optimizing several other...transient leaf transformation: Check if there are working protocols available for your species This will ... your tissue. Run a control with Agrobacterium lacking the GUS gene since plants can have endogenous GUS-like.... Successful infiltration can often be seen by sinking of the tissue (see Figure 3). This method is probably...GV3101(pMP90), which works well for us. Use a well working vector system This allows you to quickly assemble... you started. Once you have established a well working protocol in your lab, it can become a strong tool...

  2. How to Be an Excellent Trainee

    Type
    Blog Post
    ... you’re an oldbe trainee!) can be pretty nerve-wracking. So how can you be the best trainee possible? ...articles on building successful mentor relationships, asking for mentor support, and choosing your mentor. What...And I mean everything! The bare minimum should be taking notes on general protocol steps and other tips ...the moment….but you’ll probably forget and end up asking your mentor later if you don’t write it down. And... to your mentor. Pro tip! Consider physically walking through the protocol, by going to each space in...tell a trainee is understanding and things are ’clicking’ for them. It’s often easiest to train individuals..., What Now? Will You Be My Mentor? Finding and Asking for Mentoring Support   ...

  3. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...the clock for others to dive in. The real cost of making data accessible A more reasonable concern with ... the scientific community is thus not averse to making data available, but there remains concern about... by others. Other new data initiatives are also taking root. Nature Publishing Group recently launched...efficient searching and extraction of information. Looking a little further ahead, journals may move towards...Instead of providing a “dead end” figure or table, clicking on the published chart could reveal the underlying...reader as possible. Rather than adding to demands, making effective data sharing the norm should open up ...Canada where he studies protein-serine/threonine kinases implicated in human diseases. His spare time is...

  4. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...of naked DNA. Other investigators who had been working to develop high efficiency gene targeting approaches...the addition of gene-specific 1 kb homology arms flanking a FLOXed neoR gene. pAAV-SEPT-Acceptor, the preferred... CRISPR-based approaches for the generation of knockins via homologous recombination with a homologous...application - the creation of sequence-specific knockins - for which the AAV approach is most well suited...suited, since it routinely generates knockin efficiencies of 1-40% with G418-selected clones. However, there...approach requires G418 selection of infected clones, making it unsuitable for most in vivo applications.  Third... CRISPR-based approaches for the generation of knockins, it is not always ideal. Because of these limitations...

  5. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...epigenetic silencer Recombinant antibodies for marking astrocytes MagIC-Cryo-EM for sample enrichment...minimizes sample loss while also supporting imaging, making it easy to identify dozens or hundreds of particles...molar ratio) with 10 minutes of glutaraldehyde crosslinking: representative micrograph (C), particle orientation...could be overcome with methods such as chemical crosslinking or tilted-stage imaging. A truncated form of...compatible with the buffer additive MgCl2 and the crosslinking agent glutaraldehyde. Adding RvLEAMshort to ...j.cell.2021.03.025.    Recombinant antibodies for marking cell types in the nervous system By Ashley Waldron...filament expressed in astrocytes throughout the brain, making it a popular target for labeling these cells. Check...

  6. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ... pathway. Those can include cytokines and chemokines; enzymes like kinases, phosphatases, and ubiquitin...false positive signal. Dump gating When you are working with heterogeneous cell mixes, like bulk lympho... buffer solutions. Fixing causes protein cross-linking, killing the cell but preserving the cellular state...gating uses the same color for different markers, making it easy to gate on the negative cell population...investigating negative regulators of T cells and cytokine signaling in tumor immunity. When he's not in ...

  7. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...in vitro kinase reactions. Often, however, researchers lack knowledge of the regulatory kinase, access ...access to active kinase for the reaction, or have difficulties with kinase specificity in vitro. The establishment...phosphorylation sites. If knowledge of a candidate regulatory kinase is known, it’s possible to generate non-phosphorylated...phosphoserine into recombinant proteins, such as the kinase MEK1 (5). MEK1 is inactive without requisite dual...create a pSer/phosphomimetic dual mutant) elevated kinase activity over 70-fold over dual phosphomimetic ...

  8. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    ...every day, sharing ideas for the company, but also talking about sports, movies, and just about anything. ...cite lunch as one of their favorite parts about working at Addgene. We’re a small non-profit, so we can...Chef-style competitions Every few months, we’ll have a cooking competition featuring a selected ingredient. Anyone...cook-off last week, and the featured ingredient was pumpkin. Everyone gets to vote for their favorites, and...the stiff competition or says something about my cooking.   This is a creative (and delicious) team building...work better and keeps us smiling even when we’re packing the 400th plasmid that day.   As we head into Thanksgiving...

  9. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    ...Does this sound familiar? Well, when it comes to working with Addgene, we certainly hope not! One of our...email. You need help right at the moment you’re looking for it, or you may just prefer to troubleshoot ...icon, search for answers, and choose a result. By clicking on the result links themselves right from within...ll navigate to articles answering your questions making it easier than ever to get the support you need...some reason you can’t find the answer that you’re looking for, or you just have a general question or concern...valuable feedback. This is Addgene after all, and making your research easier always comes first. Resources...

  10. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...have an opportunity to improve my networking with mentors who are working in USA and Europe, something that...who migrated from their country some years ago looking for their career improvement) and bright students...world in flux. The professional development and networking resources offered by programs like IMP USA-Europe...Institute of Technology, Boston University, Johns Hopkins University, the Salk Institute, and the Karolinska...and contacts with Spanish laboratories to start working on projects together. List of discussion topics...Addgene Read Other Career Blog Posts Read the Networking for Scientists Series Read our Blog Post about...
Showing: 281 - 290 of 938 results