... without scrolling up and down the page.  Figure 3: The Education & Tools menu Further down What happens...straight from the new search bar, but the menu just above it can help you quickly and easily navigate to educational...down? You’ll find Blugene, as well as information about Addgene, our mission, links to our deposit, request...

...:02 : Niroshi and Ben introduce themselves 2:03 - 3:22 : How did you end up becoming a graduate student...graduate school community to help keep you excited about coming into lab even when things get tough in the...

...transfer plasmid with the E1 and E3 (Early 1 and Early 3) genes deleted. These genes’ functions are rescued...Degeneration in Tissue Culture. Proc Soc Exp Biol Med. 84(3), 570–573. https://doi.org/10.3181/00379727-84-20714...this introduction gives a sense of what AdVs are about – and we hope that it inspires further use of existing...

...Originally published Mar 3, 2016 and last updated Apr 13, 2021 by Will Arnold. Although CRISPR systems...a subsequent curing protocol that requires only 2-3 hours incubation. E. coli and T. citrea scarless editing...the QCascade DNA-targeting module with the crRNA, (3) three transposase proteins, and (4) the donor DNA...CRISPR Featured Topic Page Learn more about Cpf1 Find out about CRISPR Multiplexing Resources on Addgene.org...coli and other bacterial species. Read on to learn about the tools available for bacteria and some of the...that can be used in this way. As described in the above CRISPRi section, the Marraffini Lab published a ...as a collection of organisms, their genes, and metabolic processes, occupying the gut. Many human diseases...

...228, no. 2 (2012): 267-75. PubMed PMID: 22767332.  3. Kim, Dohoon, Chun-Hyung Kim, Jung-Il Moon, Young-...PubMed PMID: 19337237. PubMed Central PMCID: 2677165. 3. Jia, Fangjun, Kitchener D. Wilson, Ning Sun, Deepak... deriving human iPS cells." Nature Methods 7, no. 3 (2010): 197-99. PubMed PMID: 20139967. PubMed Central...methods do you have experience using? Let us know about your iPSCs experiences in the comments below! References...Additional Resources on the Addgene Blog Learn about Methods to Reprogram Peripheral Blood Cells Check...

...primarily show a protein’s presence or absence. Figure 3: A western blot detecting proteins using the indirect...are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments, each recognizing...cells isolated from animals immunized as described above are used to create hybridomas, which produce large...different epitope, bound by short peptide linkers. Diabodies can be used for assembling protein nanostructures... Includes: Flow cytometry Read on to learn more about the applications common to each category, including...controls, and any special considerations to think about. For step-by-step instructions of many of these ...protein of interest, as described in the IP section above. Reverse the crosslink, isolate DNA, and perform...

...on the future of the scientific endeavor October 2-3, 2014 Boston University Jacob Sleeper Auditorium, ...doing science in some form? There is a lot of talk about the state of scientist training in the US and around...

...individual, sharp and evenly spaced peaks …like these…. 3. Expect to get 500-700 bases of clean reliable DNA...and troubleshooting your DNA sequencing results Above (top right corner of this post) is an example of...

...are 24 variants of the cas14 gene that cluster into 3 subgroups (cas14a-c). All these variants share the...Additional resources on the Addgene Blog Learn more about DETECTR Browse all of the CRISPR blog posts Download...

...Method with High Throughput Capability." PLoS ONE. 3(11): e3647 (5 November 2008).  ...protein,” he says. “There is nothing you can do about it but be gentle. If it no longer works, just trash...