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Showing: 281 - 290 of 532 results
  1. Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis

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    Blog Post
    ...startups, and real applications of SynBio. Chapter 3: Synthetic biology as the engineering of biology at...synthetic yeast chromosome. Listen to learn more about Dr. Ellis, synthetic biology, synthetic chromosomes...Additional Resources from the Addgene Blog Learn about the Synthetic Biology Education Nonprofit BioBuilder...BioBuilder Plasmids 101: Golden Gate Cloning Learn About Working at a SynBio Startup in Our Interview with...
  2. Addgene's Website Update

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    Blog Post
    ... without scrolling up and down the page.  Figure 3: The Education & Tools menu Further down What happens...straight from the new search bar, but the menu just above it can help you quickly and easily navigate to educational...down? You’ll find Blugene, as well as information about Addgene, our mission, links to our deposit, request...
  3. Grad School Advice Part 1: Picking a Lab and a Project

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    Blog Post
    ...:02 : Niroshi and Ben introduce themselves 2:03 - 3:22 : How did you end up becoming a graduate student...graduate school community to help keep you excited about coming into lab even when things get tough in the...
  4. An Introduction to Adenovirus

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    Blog Post
    ...transfer plasmid with the E1 and E3 (Early 1 and Early 3) genes deleted. These genes’ functions are rescued...Degeneration in Tissue Culture. Proc Soc Exp Biol Med. 84(3), 570–573. https://doi.org/10.3181/00379727-84-20714...this introduction gives a sense of what AdVs are about – and we hope that it inspires further use of existing...
  5. QC Sequencing Technologies at Addgene

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    Blog Post
    ...from the synthesis-based sequencing methods (Figure 3). It uses (aptly named) “nanopores” that are attached...based on these characteristic drops. Figure 3: Overview of Nanopore sequencing technology. Created...sequencing was considered the gold standard until about 2008 when a new, higher-throughput sequencing-by-synthesis...
  6. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

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    Blog Post
    ...Originally published Mar 3, 2016 and last updated Apr 13, 2021 by Will Arnold. Although CRISPR systems...a subsequent curing protocol that requires only 2-3 hours incubation. E. coli and T. citrea scarless editing...the QCascade DNA-targeting module with the crRNA, (3) three transposase proteins, and (4) the donor DNA...CRISPR Featured Topic Page Learn more about Cpf1 Find out about CRISPR Multiplexing Resources on Addgene.org...coli and other bacterial species. Read on to learn about the tools available for bacteria and some of the...that can be used in this way. As described in the above CRISPRi section, the Marraffini Lab published a ...as a collection of organisms, their genes, and metabolic processes, occupying the gut. Many human diseases...
  7. Delivery Methods for Generating iPSCs

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    Blog Post
    ...228, no. 2 (2012): 267-75. PubMed PMID: 22767332.  3. Kim, Dohoon, Chun-Hyung Kim, Jung-Il Moon, Young-...PubMed PMID: 19337237. PubMed Central PMCID: 2677165. 3. Jia, Fangjun, Kitchener D. Wilson, Ning Sun, Deepak... deriving human iPS cells." Nature Methods 7, no. 3 (2010): 197-99. PubMed PMID: 20139967. PubMed Central...methods do you have experience using? Let us know about your iPSCs experiences in the comments below! References...Additional Resources on the Addgene Blog Learn about Methods to Reprogram Peripheral Blood Cells Check...
  8. A Conference By Postdocs For Postdocs: Future of Research

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    Blog Post
    ...on the future of the scientific endeavor October 2-3, 2014 Boston University Jacob Sleeper Auditorium, ...doing science in some form? There is a lot of talk about the state of scientist training in the US and around...
Showing: 281 - 290 of 532 results