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We narrowed to 818 results for: cell

Showing: 281 - 290 of 818 results

  1. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...co-expression among cellular markers You’ll also need to know if markers are co-expressed in your cells when designing...immunologists, allowing for the rapid analysis of cell populations within a heterogenous tissue type, such...methods, the identification and isolation of rare cell types. In recent years, full spectrum flow cytometry...Spectral flow allows for autofluorescence extraction Cells produce a low level of fluorescence called autofluorescence...single color control. Reference controls: Beads or cells? Compensation beads are a convenient tool to obtain...you prepare reference controls of both beads and cells for a single fluorophore. Although compensation ...Additionally, it is critical to include unstained beads or cells within your reference group to ensure proper unmixing...

  2. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    ... Ba/F3 and MCF10A cellular models. Ba/F3 and MCF10A are growth factor dependent cell lines and any mutations...attempting to characterize mutations in a pooled cell population, as mutations with strong phenotypic ...functional groups, based on how each one impacted cell viability (see figure). Most of the mutations were...to be “neutral,” having no significant effect on cell viability compared to a wild-type control. Approximately... the mutations, however, significantly increased cell viability, and these “activators” may be the direct...annotation of somatic mutations in cancer." Cancer Cell 33.3 (2018): 450-462. PubMed PMID: 29533785. PubMed...

  3. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...fluorescence images of C2C12 cells stably expressing pTRE-Tight-MitoTimer and rtTA. The cells were pulsed with 2µg... Robinson et al., Dev Cell 2010 Feb 16;18(2):324-31. Cheeseman et al., J Cell Sci 2013 May 1;126(Pt ...neighboring cells in imaging applications and the mapping of neuronal projections to their associated cell bodies... targeted to subcellular regions or protein complexes for proteomic mapping in live cells. Lam et al...been used by several groups to monitor protein and cell ageing, as the spectrum shift occurs during Timer...(pTRE-Tight-MitoTimer) to show its usefulness in cell culture to report changes in mitochondrial turnover...rapamycin-binding domains, such as FKBP and FRB. The cell does not appear to mind extraneous proteins bound...

  4. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...activity. These cells, presumably the cell you want, cannot break down X-gal and are white. Cells that don’t...killing off a portion of the cells like in a selection. Instead, all cells survive and you will need to... the plasmid you’re looking for out of the many cells that don’t. One way to begin the search is by using...using selection strategies, where only cells that have gained or lost a specific gene survive (ex: antibiotic... plates containing X-gal allows you to identify cells that took up the vector and distinguish between ...

  5. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...with) the cells’ own natural translation machinery; these are often incorporated into the cells via plasmid...published, followed soon by GCE done in mammalian cells (Shandell et al., 2021).   How it works If you’...is usually added to the media for uptake by the cells. Second, you’ll need tRNAs and tRNA synthetases ...biosynthetic pathway for the ncAA integrated into the cell’s genome, instead of incorporation via plasmid or...Theoretically, if you put all these together, the cell will produce a protein with a site specifically ...techniques are straightforward, the systems – the cells growing and producing the proteins – are not, and...component of the experiment system (including media, cell type, and growth temperature) may need to be individually...

  6. Hot Plasmids - February 2022

    Type
    Blog Post
    ... including live-cell imaging, single-molecule tracking, immunolabeling of fixed cells, Western blotting...possibilities in both cell engineering and gene therapy.  Xiaoshu X, et al. Molecular Cell. 2021. https://doi.org...well-folded scFv scaffold to improve its stability in cells, and use it in a variety of applications. This set...frankenbodies to amplify the signal.  Liu et al. Genes Cells. 2021. https://doi.org/10.1111/gtc.12893, Zhao et...Cas12a, creates challenges for in vivo delivery and cell engineering applications. CasMINI, a new miniature...endogenous genes. It is highly specific in mammalian cells without significant off-target effects, yet small...Anti-FLAG Frankenbodies for modular imaging of cellular targets Optimized genetic screening technique ...

  7. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    ...through programming cells. In the context of therapeutics, if you want to program a cell to identify and ...translational activity a cell has and control how much it taxes the resources of a cell. The objective is to... to the regulatory networks within E. coli host cells. Image from Nielson and Voigt (2014). ...and target a malignant cell, then you’d need the ability to turn on genes at different times. It requires...being used. One thing is that Cas9 is toxic. When cells carry CRISPR circuitry, it can slow their growth...chemicals as sophisticated as those that living cells and animals make. That will be an outcome of genetic...within the wood. There are different types of cellulose fibers, pattern, shape. All of that is controlled...

  8. Working with Nuclear Receptors

    Type
    Blog Post
    ... that are diffused or actively transported from cells, traverse the body through the bloodstream, and ...corepressors depending upon a context defined by ligand, cell type, and promoter, organizing the expression of... coactivator proteins individually contribute to cell regulation in a coherent manner. Because coregulators...that coactivators may be integrators of multiple cell signaling systems. Activation of membrane receptors...sense the impact of the total environment on the cell. The NURSA Consortium Now in its third phase of ...purification proteomic profiling of coregulators in HeLa cells was the first comprehensive analysis of the interactome...executioners of transcriptional regulation. Molecular Cell 27, 691-700. (PubMed) McKenna, N. J.and O'Malley...

  9. AAV Molecular Tools

    Type
    Collection
    ...mapping 5 Kim Tools for Cell Ablation These AAV encode tools that can be used for cell ablation. ID Name Expression...Transactivators Affinity Purification Neurophysiology Cell Ablation Genome Engineering Labeling Overexpression...diphtheria toxin receptor fused to GFP for studying cell ablation. 2 Jessell , Azim 45580 pAAV-flex-taCasp3...expression of designer pro-taCasp3 and TEVp for studying cell ablation. 1, 5 Shah , Wells Genome Engineering Tools...Tools These AAV encode tools that can be used for cell or protein labeling. ID Name Expression System Activity...

  10. Retrovirus Plasmids

    Type
    Collection
    ...reverse transcriptase and integrase. Once in a target cell, the virus uses the reverse transcriptase in tow...γ-retroviruses are that they can only transduce dividing cells (this is because they are only able to enter the...of Large T antigen for creation of immortalized cells (see Weinberg Lab page for resistance variants) ...Expression of hTERT for creation of immortalized cells; additional plasmids include Puro resistance and...inducible expression of GFP T2A Cre fusion in mammalian cells Foijer 47916 pRXTN CMV/MSV pRXTN is a modified version...MoMSV Stable expression of luciferase in mammalian cells Wong Return to Top Do you have suggestions for other...
Showing: 281 - 290 of 818 results