We narrowed to 997 results for: lars;

... are particularly good models for building chromatin interaction networks since they are a large cluster...biotinylated by BirA. From here, the CAPTURE method is similar to other 3C methods: crosslinking captures chromatin...Additionally, CAPTURE 3C-based sequencing requires a large number of cells (~5x107). Together, these two requirements...well-characterized beta globin locus. The two methods had similar rates of specificity for the DNA sequence targeted...interactions identified with the multiplexed CAPTURE 2.0 largely replicated interactions previously identified with..., like those that occur during development and cellular differentiation. Using a cell line model, the ... for spatial genome organization deciphering. Molecular Cytogenetics, 11. https://doi.org/10.1186/s13039...

...at the molecular level, at the cellular level there is a universal theme - uncontrolled cellular proliferation... no other disease receives as many billions of dollars in funding or as much attention collectively from...causes of this uncontrolled proliferation, in particular the roles of proto-oncogenes, tumor suppressors...the accelerator of a vehicle being stuck down. Similarly, a mutation that turns a tumor suppressor “off...universally-dividing behavior of cancer cells, attacking the cellular machinery necessary for division. This approach...research has focused on picking apart the complex cellular pathways involved in the development of different...

...Find basic molecular biology protocols Find plasmid cloning protocols Check out our molecular biology reference...one-pot” reaction to form complex, multi-insert modular assemblies that enable biosynthetic pathway engineering...destination vector receives the assembly after similar Type IIS restriction enzyme cutting results in ...performed additional efficiency measurements using similarly sized Lambda amplicon inserts to control for any.... To compensate for this, researchers can plate larger volumes of the outgrowth containing the transformants...assembly. The fidelity data can be applied to derive similar high-fidelity overhangs for any Golden Gate assembly...England Biolabs, Inc.         Eric Cantor is a molecular biologist and a Development Group Leader overseeing...

...single base pairs, to chromosomal inversions and large deletions. Many of these methods rely on Cas9 or...Although seemingly ubiquitous in nature, they are largely underutilized in research settings — mostly a consequence...is not as well understood but likely occurs in similar regions. This combination of chewing up one stand...stand and cutting the template strand causes large deletions of up to hundreds or even thousands of base...proteins naturally bound to the DNA. Cas3 or Cas9? Large genomic deletions can be achieved using Cas9, but...but Cas3 may be a better option. To get large deletions using Cas9, two or more gRNAs must be used along...Cascade-Cas3 complex in nucleotide unwinding steps. Molecular Cell, 70(3), 385-394.e3. https://doi.org/10.1016...

...and Biosensors: Throwing color lights on molecular and cellular processes” Curr. Protein. Pept. Sci. 9(...discovery, the family of FPs just keeps getting larger with hundreds of variants available. Read on to.... Therefore, these can be used as “timers” for cellular processes following their activation. The four... called Slow-FT, Medium-FT, Fast-FT, and mK-GO. Large Stokes Shift (LSS): Stokes shift (named after George...split FP. This technique is also is also called Bimolecular Fluorescence Complementation (BiFC). 8 points...The maturation of the chromophore on many FPs (particularly those derived from GFP) requires oxygen. Therefore...interested in everything related to gene expression, particularly at the RNA level. He maintains the greenfluorescentblog.wordpress.com...

... activated at a similar spectrum as HR and also results in membrane hyperpolarization but unlike HR, this...Simon! Derek P. Simon received his PhD in Cellular and Molecular Biology from the University of Michigan...neuron in a specific brain region can result in a particular neurotransmitter release or post-synaptic current...the kinetics of the opsin variant, and other particularities that best suit your specific experiment. Consult...cation channel, activation of which results in depolarization and subsequent firing of an action potential...results in efflux of chloride ions, subsequent hyperpolarization, and inhibition of action potential generation...crossed with an appropriate mouse line expressing a particular cre, viral expression of your opsin is no longer...

...the most popular and useful viral vectors in the lab. Advantages of lentivirus include a large genetic ...genetic cargo (8 kb vs 4.5 kb), and they remain popular for targeting and tracing cells in the brain (Figure...vectors in the lab Lentiviral vectors are incredibly popular in the lab - the Trono lab’s lentiviral packaging...the clinic Since lentiviral vectors can deliver a large amount of DNA (~8 kb) with a relatively low immune...lentiviral vectors for applications that require the large genetic capacity of a lentiviral vector but for ...PubMed PMID: 23322669. 6. Genetic treatment of a molecular disorder: gene therapy approaches to sickle cell...Dorazio R, Mulder GJ, Polack A, Bank A, Soulier J, Larghero J, Kabbara N, Dalle B, Gourmel B, Socie G, Chrétien...

...minigenomes are incapable of producing infectious virus (similar to lentiviral vectors) because they do not encode...components of the Zaire ebola virus minigenome system Similar to other RNA viruses, EBOV brings its own RNA-dependent...which is formed by a complex of two proteins: the large (L) catalytic protein and its cofactor VP35. VP30...transcribed (and the viral mRNAs are translated) by cellular machinery. NP encapsidates the negative sense,...necessary control elements for each would be relatively large. Cloning the viral genes in this plasmid would be...polyadenylate the mRNA. These mRNAs are translated by cellular host machinery (as viral mRNAs would be in the...processes in extreme detail. The mechanism of a particular observation can be easily studied as each component...

...the entire proteome of a particular cell type, or a subset based on a cellular compartment or growth stage...Obrdlik, P., El-Bakkoury, M., Hamacher, T., Cappellaro, C., Vilarino, C., Fleischer, C., Ellerbrok, H., Kamuzinzi... and are still used extensively to screen for molecular interactions in the cell, including protein-protein...activities (1). Researchers also discovered the modular format of some transcriptional activators: that... yeast two-hybrid (Y2H) system as a robust and popular lab tool. In the classic two hybrid system (also...two-hybrid systems are a powerful tool for screening molecular interactions, accuracy and reproducibility are...

...one (admittedly large) class of common fusion protein tags. Biochemists and molecular biologists who need...challenges, researchers have amassed a considerable molecular tool box of tags and fusion proteins to aid in... interest to overexpress a protein. Energy and cellular resources are being spent to make something the...small sequence of amino acids, is basically a molecular leash for your protein. If you’re working with...; has inherent enterokinase cleavage site GST Large Protein 26 Purification and Stability Good for purification..., flanked by hexahistidine motifs (6xHis) MBP Large Protein  40 Solubility and Purification Can improve...Solubility and Affinity Abundance of charged and polar residues improves solubility; good for antibody-...