We narrowed to 643 results for: ATO
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TypeBlog Post.... The pDonor plasmid pRE57-Ter with multiple terminators can serve to interrupt genes (or gene clusters...
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Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog Post...several years ago – recently collaborated with Senator Barbara Boxer to introduce the Alan Reinstein Ban... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post...paid. Institutional salary policies are one indicatator of how salaries may changes due to the FLSA. ... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express... -
Plasmids 101: Control Plasmids
TypeBlog Post...hypothesis: proper controls! For every experiment, an investigator needs a standard against which the results can... -
Lab Safety for Biosafety Levels One and Two
TypeProtocol... PPE protocols . Working in a BSL-2 laboratory requires laboratory glasses in addition to coat and gloves... eat, drink, chew gum, or apply makeup in the laboratory. Before you start your experiment, make sure ...Only mechanical pipetting should be done in the laboratory. Don’t mouth pipette! There may be some times...officer. BSL-2 Guidelines Remember, the BSL-1 laboratory guidelines above are expected to be followed ...not be worn outside of the BSL-2 area. BSL-2 laboratories must be clearly marked as “BSL-2.” The names...names and contact information of the laboratory manager should be clearly visible in the room and on the ...vaccination or titer prior to starting work in the laboratory. For some biohazardous waste, an autoclave or... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog Post...et al. "Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...10% expression in primary human T cells and hematopoietic stem and progenitor cells (HSPC)s to ~40% expression... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog Post... combine pairs of mutations together to test combinatory effects. One-pot saturation mutagenesis The ... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...25831507 PubMed Central PMCID: PMC4394297. 16. Iwano, Satoshi, et al. "Single-cell bioluminescence imaging of... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog Post...lab (user, date, time, action) are required for regulatory purposes in many labs. Besides this being of ... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog Post..., and enzymes for cancer, autoimmune, and/or inflammatory therapeutic applications. Antibody fragments... -
Filming Science Videos in the Age of Social Distancing
TypeBlog Post... their mask. But while the camera is here, the operator is in a different room. Design: The previous... -
Hot Plasmids: Summer 2024
TypeBlog Post...dCas9, zinc finger proteins, and transcription activator-like effectors (TALEs), with fifteen total variants... -
Antibodies 101: Beyond Surface Labeling
TypeBlog Post.... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity... -
Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog Post.... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog Post...Spatial Proximity." Journal of Investigative Dermatology 137.12 (2017): e197-e203. PubMed PMID: 29169465... -
Site Directed Mutagenesis by PCR
TypeBlog Post...introduce or remove restriction sites), in mapping of regulatory elements (to mutate promoters/enhancers in reporter... -
Evolution of Lab Techniques
TypeBlog Post...DNA technologies that are now commonplace in laboratories. As scientific technologies rapidly progress... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog Post...loop (as might be anticipated to occur within a terminator sequence), since this would directly compete ...