...force to control protein expression in mammalian cells: easy to use biological tools are abundant! In this...tried-and-true protein expression control tools as well as explore some new innovations. Whether you want...tag prior to introduction of the construct into cells. However, if you would like tunable control of an...

...enters in the middle of the action. If the paper is well-written, the abstract should give you a sense of... a few different ways to automate the process as well. Sign up for eTOC (electronic table of contents)...searching and sorting your own digital library as well as some integration with internet-wide literature...

...generated beautiful figures. And, you can’t wait to tell your story. But, before you can show off your work...the most common poster size, but this can vary as well.  Sections of your poster In almost all scientific...there are some particular styles recognized to work well. It is important that your readers can navigate ...

... PAM constraints just won’t cut it anymore. Say hello to PAM-free and PAM-flexible nucleases! In this ... for off-targets, secondary structures, etc., as well as the presence of a Protospacer Adjacent Motif ...Precise and efficient scarless genome editing in stem cells using CORRECT. Nat Protoc 12, 329–354 (2017). DOI...

...advice is always "don't do it unless you can do it well" – even the smallest program. I don't mean have ...have all been there. A good panel discussion is a well choreographed performance with room for fortuitous..., but the organizers and moderator should have a well-planned approach to making the event interesting...

...held your favorite protein in the palm of our hand? Well, actually – have you ever held a model of your favorite... the real thing. Nevertheless, the models are compelling, and I encourage you to hold one. Why do we ...protein might represent one aspect of the protein very well, it will almost certainly fail to convey other aspects...

...It is a well known fact if a multicopy variant of a gene exists, there must be assays that researchers...the genome that are difficult to accurately read (Bell, 2018.) Even though it does not require a standard...Reference Molecular Cloning Techniques References Bell AD, Usher CL, McCarroll SA. Analyzing Copy Number...

...visible whether expressed in E. coli, in mammalian cells, or in purified form, and didn’t need any added ...like quantum chemistry and protein biophysics, as well as inventors and biomedical engineers, join efforts...References Duret, G., Polali, S., Anderson, E. D., Bell, A. M., Tzouanas, C. N., Avants, B. W., & Robinson...

... by Kevin Esvelt, a Wyss Technology Development Fellow at the Wyss Institute and Harvard Medical School...with appropriate flanking homology into a germline cell can create an RNA-guided gene drive. In principle... experiments2,3. While that didn't work quite as well as anticipated4, greater awareness will certainly...

...new pipette tip for each step and mix the samples well by vortexing or inversion. Created with BioRender.com...values and the predicate values and measures how well the data points fit the trendline. R2 ranges from...change pipette tips regularly, and mix samples very well.  Use duplicates or triplicates to increase the ...

...guidelines here. A detailed transformation protocol, as well as protocols for growing Arabidopsis are also available...incubated at stationary phase for too long, the cells in your culture will no longer be viable. The time...graduate student at Stanford University. She studies cell fate maintenance in the stomatal lineage and has...

...attend. Societies, such as the American Society for Cell Biology (ASCB) or the American Chemical Society ...looking for work in today’s job market should have a well-groomed LinkedIn page. Here you can search for jobs...someone professionally that you do not know very well; always ask for information and establish a connection...

... the 926 video abstracts identified. The journal Cell had the largest average view count per video abstract... on sharing your research through video. Liz Haswell and Eric Hamilton, biology professors at the University.../wesharescience.com/pin/7472 Article: http://www.cell.com/current-biology/abstract/S0960-9822(16)30873...

...others may feel they can share opposing opinions as well. I am sure we have all experienced a meeting where...stop you from speaking up.  If your ideas are not well received, understand that people can be resistant... as many solutions as you can in that timeframe. Tell yourself they don’t have to be perfect or even good...

... . Steps of an IP: 1) Protein extracts from cells or tissues contain a complex mix of proteins seen...common immobilization methods utilizes bacterial cell wall proteins, Protein A and Protein G, which bind...does not. Common negative controls include knockout cell lines or tissues that do not express the protein...

...But ask any filmmaker or videographer and they’ll tell you, a lot of the fun is building within the constraints...Some came in even lower.  Learn: While this works well for social media videos, we need as much detail ...stopping recording).  Test: It was unbelievable how well this worked! Interviewees found it easy to drop ...

...established theory. Realizing that textbooks don’t tell us everything there is to know is a strange and ...be the first person to make a discovery. I can’t tell anyone else what the “correct” motivation to do ...recommend talking to other undergraduate researchers, as well as graduate students (your teaching assistants),...

...to provide researchers with a collection of IPI’s well-validated and ready-to-use recombinant antibodies...of Addgene’s recombinant antibody collection, as well as access to IPI educational resources developed...

...developed and widely adopted in the past few years, as well as refreshing the existing content. Access the CRISPR...more flexible requirements and enhanced editing, as well as alternatives to Cas9. All of this is accompanied...

...microbiology resources Stem cell models for disease and open science: Interview with Darrell Kotton Find additional...

...them with your coworkers, labmates, and friends as well!   Addgene is sharing so much more than plasmids...via GIPHY   Or maybe your experiment went so well you need to express yourself. We have something ...

...History Rap Battle A great example of science story telling following the unfortunate story of Rosalind Franklin...the African American woman whose cervical cancer cells became a mainstain of biological study without her...

...Sleigh bells, please! It's time to announce the winners of the 2022 #DeckTheLab contest!  and it's.....y34Tv8VCLT— Borris Aldonza (보리스) 🇰🇷🇵🇭 (@PrecursorCell) December 12, 2022          View this post...

..., a Clinical Fellow in Pathology at Masachusetts General Hospital and Postdoctoral Fellow in the Church...

...co-founder of Addgene. Melina received her Ph.D. in Cell Biology from Harvard University, where she studied...Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization ...

...selection markers (for use in other cell types, e.g. eukaryotic cells). pBACKBONE-XXXXX Tip: For a catalog...

...publication, you'll find that information in the report as well.    Figure 3: An antibody data report, showing ...report, you’ll find a results section, which will tell you if the antibody worked under the conditions ...

... sure that the world will go on without my whole-cell extract from 2009. Join a newsletter to stay up-...environment, but most likely safer for researchers, as well. Additional resources on the Addgene blog Learn...

...include popular envelope and packaging plasmids as well as transfer plasmids. Download the orange lentivirus...instead, don’t fret; we have backgrounds for you as well! If you use FRET, you might be interested in our...

...happens if you do scroll down? You’ll find Blugene, as well as information about Addgene, our mission, links...moment to let us know what you think, through the yellow popup at the bottom of the page. Happy surfing!...