We narrowed to 931 results for: TIM

...desired edit to still occur if the cut site is sub-optimal? As with everything in biology, the answer is always...

... The Junwei Shi Lab addressed this problem by optimizing the Cas protein delivery with Peptide-Assisted...

...genome of an hiPS cell. While the majority of the time the genome will attempt to repair itself without...

...the MTA. Pro Tip! Download a new spreadsheet each time you want to submit new plasmids! We update the spreadsheet...

...allowing for rescued expression of GeneR.   Sometimes also referred to as DiO (Double-floxed inverse...

...contributes to variability in RNA half-life, and ultimately gene-expression. Polyadenylation is the post-...

...so far! Why do we need PAM flexibility? IDT estimates that there is an NGG PAM every 42 bases in the...

... Editing by John Doench CRISPR-Cas9: Tips for Optimizing sgRNA Activity by John Doench All CRISPR Posts...

... maintaining high brightness and fast maturation time (Figure 1). See the paper for more details on small...

...of course, only hypothetical at this point. More time and energy must be devoted to studying Cas3 and ...

...expressed in non-dividing cells for long periods of time. This reduces the chance of integration at random...

...before moving onto your experimental samples. With time and practice, you may be able to do your compensation...

...protospacer. The resulting double strand break (DSB) stimulates DNA repair mechanisms that can be exploited to...

...available through Addgene, with more being added all the time. Keep in mind that libraries are only as good as...

...appropriate immunizing reversal drive at the same time to mitigate the potential effects of an accidental...

...activate the immune system, and of the infection to sometimes result in cell death, has led to interest in using...

...antibodies from its serum, and meticulously screening, optimizing, and characterizing several anti-mouse and -rabbit...

...directed evolution across the full ADAR2DD to further optimize cytidine deaminase activity. While RESCUE can ...

...ancestry group. With the existing methodology, they estimate that the African ancestry-associated cancer dependencies...

...titration experiments might be helpful to achieve optimal results. It is important to note that this system...

... that a construct is functional before investing time and resources into making a stable cell line. When...

...episomal vector system generated by the group is an optimized mixture of three episomal plasmids (Table 1) encoding...

...by nonspecific complement mechanisms in serum. Sometimes this is due to the cells that the viral vectors...

...experiments. They’ve also helped researchers design and optimize cloning methodologies using counterselection. ...

...libraries Overview of Library To better define the optimal conditions for CRISPR knockout screens, the Bassik...

...peripheral nervous system (PNS) targeting AAV  Get 1st time AAV user tips from an AAV rookie  Learn about some...

...Gail Mandel and her team have shown for the first time that programmable RNA editing can repair a gene ...

...and Bagpipe. How does a gene get its name? Often times, a gene gets its name based on what happens to an...

...AIBS). Scientists at AIBS have allocated tremendous time and resources to generate highly specific tools ...

...vectors typically involves shuttle vectors, also sometimes called transfer vectors. The gene of interest ...