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We narrowed to 1,025 results for: URE

Showing: 841 - 870 of 1025 results

  4. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...need to be able to replicate in Agrobacterium to ensure that they are maintained during co-cultivation ...plasmid can also be customized due to its modular structure. Two multiple cloning sites (MCS's) flank the ...

  6. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...Publication policy with two changes that will help us ensure that plasmids don’t get left behind in this status...Addgene! We'll continue to review our policies to make sure they meet our goals, maintain your trust, and meet...

  7. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ... a closer look at the rep and cap genes. Figure 1. The RepCap plasmid and its products. Created...265 . https://doi.org/10.1016/j.omtm.2019.01.008  Maurer AC, Weitzman MD (2020) Adeno-Associated Virus Genome...

  9. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...with the small solubility tag GB1. In SunTag nomenclature throughout the rest of this blog post, GFP refers...the GCN4 sequence to increase its alpha-helical structure and stability, creating v4 SunTag. Since the v4...

  11. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ... introduce genes into an organism’s genome (see figure) and to disrupt endogenous genes at the site of...antibiotic-resistance genes conducted in HeLa cells). In their Nature Genetics paper, the lab showed that efficiencies...

  12. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ...mechanisms for feedback (more on this below). Make sure to give positive feedback when it is merited. Surprisingly...unnecessarily big issue for you and for the employee. Make sure to focus on the action, not the person - intent ...

  15. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...new. Science involves success and, more often, failure. It’s useful for non-scientists to understand not...science and society 6)   Consider participating in a future SciFund Challenge. While the goal is ostensibly...

  16. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ... been difficult to modify in the past.   Figure 1: Identification and enrichment use of XMAS-TREE...splice acceptor and donor sites, and introducing premature stop codons.    New from the viral service By...

  17. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ... tool. They can search the database by certain features like serotype, promoter, or tool type and each...use these questions as a guide for designing your future injection experiments. Have your data or lab notebook...

  21. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...through biotin-avidin binding could provide a true measurement of high affinity (i.e. sub-nanomolar) protein-ligand...map and cartoon below). Including biotin in your culture and inducing protein expression with IPTG results...

  22. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...questions help us expand and improve our resources for future users. We’re excited to be distributing new types...fulfilled more than 140 orders for virus! We’ll be sure to keep you updated on our progress and would love...

  23. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...spatiotemporal resolution required for precise in vivo measurements of dopamine (113049, 113050) or norepinephrine...Looger’s lab at Janelia for neuroscientists to measure glutamate levels. iGluSnFR.A184S, a variant with...

  25. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...interspaced with linkers that can fold into a secondary structure that can bind specific proteins via aptamer domains...using RNA scaffolds to assemble proteins in vivo. Nature protocols. 7(10) 1797-1807 (2012). PubMed. ...

  27. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ... Julie from Novartis who was working with cell culture and who was extremely helpful since she was familiar...useful pieces of information from the workshop. Future workshops for Addgene Addgene strives to be a great...

  28. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...? Check Out Our Plasmids 101 eBook! One of the features that makes NEBuilder HiFi so accurate (apart from...incorporation of a 3’-5’ exonuclease in the reaction mixture. The exonuclease removes mismatched 3’ overhangs...

  29. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...modified Tango beta-arrestin recruitment assay to measure receptor activation for more than 300 GPCRs. In...peroxidase-catalyzed proximity labeling to quantifiably measure GPCR agonist response in vivo (Paek et al., 2017...
Showing: 841 - 870 of 1025 results