Plasmids 101: Screens vs. Selections
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Blog Post
...raising or lowering the culture temperature to exacerbate a mutant phenotype, very similar to enhancer/...
We narrowed to 730 results for: ACE
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Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog Post... the current global pandemic in which scientists race to find vaccines, therapeutics, and other treatments... -
Fluorescent Proteins 101: Luciferases
TypeBlog Post...lower-energy photon; no chemical reaction takes place. Figure 2: Simplified chemical reaction catalyzed... -
Viral Vectors 101: Types of viruses
TypeBlog Post...are roomy: some adenoviruses pack up to ~35 kb of space for cargo! Adenovirus is an effective option if ... -
Viral Vectors 101: Viral Applications
TypeBlog Post...human transcription factors, barcoded libraries to trace cell lineage, and so much more! Libraries allow ... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog Post...process is fairly straightforward. Basically, you place your gene of interest in a plasmid and get the vector... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog Post...the upregulation of the immunosuppressive cell surface receptor PD-1. This immunosuppressive environment... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog Post...Learn About our Cancer Colleciton Pages Use the ClonTracer Library for Lineage Tracing Learn About Cancer... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog Post...Usually Destroyed Yes, Cpf1 cleaves 5' of the protospacer Multiplexing CRISPR-Cas9 options before Cpf1... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog Post...will help scientists Good science isn’t the only facet of a project this big. Maintaining our excellent... -
Plasmids 101: Inducible Promoters
TypeBlog Post...and allowing reporter gene transcription to take place. Addgene also has popular light-regulated two component... -
A Novice's Perspective on Neuroscience - SfN2017
TypeBlog Post...technologies that will have impacts on many different facets of life. It also seems as if many of the experiments... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog Post...Distributing hardware via globally established makerspace networks and training users via workshops, seminars... -
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog Post... and not a false negative. Therefore, they will place special emphasis on positive controls and good sample... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog Post... a much lower cost. Read on to find out how to replace your SDM method with REPLACR (Recombineering of... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog Post...last one of those gene regulatory proteins and the places in the genome where they can be found more quickly... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...the protein’s C terminus. With that new design in place, Konermann immediately saw an order of magnitude... -
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur
TypeBlog Post...to Find a Job in Biotech and Resume/CV tips - LabSpaces Blog Find a Buisness Plan Competition Good Relations... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog Post...Editor at F1000Research. With many changes taking place in the world of science publishing, and an ever ... -
Plasmids 101: Yeast Vectors
TypeBlog Post...homologous recombination to facilitate simple gene replacement/mutation. Yeast expression plasmids used in the...