We narrowed to 835 results for: ANG

...college student searching couch cushions for spare change, desperate to extract a few more photons from a...improved brightness, photostability, and spectral range. Nevertheless, these fluorophores were designed ...

... Percent of Neurons Transduced   Dorsal Root Ganglion (DRG) AAV.9 (parental) 46 +/- 0.7% AAV.PHP.S...figure 8 in Chan et al).  References 1. Chan, KY, Jang MJ, Yoo BB, et al. Engineered AAVs for efficient...

...Gateway-compatible bait Y2H plasmid pEZY202  from Yu-Zhu Zhang’s lab) is an example of the former. In this system...While this may be difficult to resolve for a wide range of prey proteins, there are now a number of two-...

... staggered fashion, creating a 5 nucleotide 5’ overhang 18-23 bases away from the PAM. With this information... Other CRISPR Blog Posts Additional Resources Zhang lab Cpf1 blog post on Benchling ...

... been converted to an alanine (D10A or H840A), changing its double-strand cut to a single-stranded nick...for your target gene.  Validated gRNAs are a great bang for your buck – they have been used successfully...

...and blue triangles. 2) An antibody immobilized on a bead binds specifically to the blue triangle protein...

...has no doubt changed drastically over the past few decades. But no matter how much it changes, it will still...

...website.   Bibliography  Ersing, I., Rego, M., Wang, C., Zhang, Y., DeMaio, K. H., Tillgren, M., Fava, A.,...

...Danio rerio) have been used since the 1930’s in a range of biological studies, including investigations ...fluorescently labeled, allowing researchers to observe changes to these tissues under different experimental conditions...

...AAV). This issue was recently circumvented by the Yang Lab with the development of a Suntag-p65-HSF1 (SPH...as well as individual neuronal populations.  The Yang Lab’s AAV plasmids used to express Cre under neuron-specific...

...mitotic cells as well as necrotic or dead cells (Jiang and White-Cooper, 2003). Why call a gene required... 139.7 (1997): 1805-1819. PubMed PMID: 9412474. Jiang, Jianqiao, and Helen White-Cooper. "Transcriptional...

... had to overcome some hesitation to ‘approach strangers’ and learn how to communicate outside my scientific...message and to keep it short. Soon, discussing with strangers stopped feeling weird and started to be fun. I...

...addition, it can be advantageous to speak multiple languages - that makes communication with customers from...requirements (and maybe also the proposed salary range) should help. In smaller companies, technical support...

...digital PCR (ddPCR) titration protocol (modified from Wang et al., 2018). Briefly, 293T cells are transduced...Article 2. https://doi.org/10.1089/hgtb.2013.131 Wang, Y., Bergelson, S., & Feschenko, M. (2018). Determination...

...watched each month, in 39 countries, and in over 54 languages. While most scientific videos were for entertainment...researchers for whom English is a second or third language (making publishing their research all the more...

... of replication (p15A) worked best under a wide range of inducer concentrations (0.1-1% L-arabinose) without...confluent well of HEK cells in a 6-well dish and change the media every day for three days. GVs should ...

...supporting datasets must be logically annotated and arranged, as in an actual publication and not require interaction...take interest in how it is released, we will be in danger of having to deliver on well-meaning but impractical...

...lamps are the best for working in this spectral range. You can also check that no filters and/or cold ...tell us about cellular events via  fluorescence changes occurring due to protein synthesis, degradation...

... Choosing the promoter Mature siRNAs typically range from 19–22 nucleotides and are 100% complementary... Taxman, D. J., Moore, C. B., Guthrie, E. H., & Huang, M. T. (2010). Short hairpin RNA (SHRNA): Design...

...for researchers looking to generate long-lasting changes in cells. However, for some applications, integration...construct has the D64V mutation, a single amino acid change (from aspartic acid to valine at position 64) which...