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We narrowed to 943 results for: Arch

Showing: 561 - 580 of 943 results
  1. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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    Blog Post
    ...efficiency of homology directed repair, with researchers hypothesizing that they can efficiently integrate...bp sequence around the desired insertion site to search for potential gRNAs. As with other homology-directed... under a Creative Commons 4.0 license. The research groups responsible for Easi-CRISPR have also recently...
  2. Hot Plasmids: Fall 2025

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    Blog Post
    ...decades since this transfer was first proposed, researchers have shown that these transplanted mitochondria...and altering the composition and dynamics. The researchers demonstrated this perturbation interrupted the...license.  In addition to chromoproteins, the researchers developed a version of the kit that uses melanin...
  3. Generating Mouse Models Using CRISPR/Cas9

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    Blog Post
    ...mouse genome. With these early technologies, a researcher would first introduce a mutation into a mouse...and rushed to test it. I still remember the day, March 16, 2014, that I got my first preliminary results...CRISPR-Cas9 is an acquired immune system in bacteria and archaea. As you know well if you’ve been following the ...
  4. Building Global Connections with the International Mentorship Program USA-EUROPE

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    Blog Post
    ...couldn’t be luckier with my mentor, since she does research at UK in the same field that I do: prostate cancer...highlights included remarks by the Spanish Secretary of Research, Development, and Innovation, D. Carmen Vela, ...    Rosario Fernandez-Godino is a Postdoctoral Researcher at MEEI-Harvard Medical School, where she studies...
  5. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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    Blog Post
    ... design of these constructs, allowing novice researchers to quickly and easily start genome editing (Schwartz...donors in cultured Drosophila cells. Nucleic Acids Research 42:e89–e89 . https://doi.org/10.1093/nar/gku289...CRISPR-targeted homologous recombination. Nucleic Acids Research 41:e193–e193 . https://doi.org/10.1093/nar/gkt805...
  6. How to Design Your gRNA for CRISPR Genome Editing

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    Blog Post
    ...CRISPR Plasmids for Your Research Find CRISPR Pooled Libraries for Your Research ... human stem cell genome editing. Nucleic Acids Research 41:9049–9061 . https://doi.org/10.1093/nar/gkt555...
  7. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    Blog Post
    ...high levels of spurious expression.  As such, researchers developed new tools called FLEx/DIO vectors where...expression levels over several weeks allows the researcher to determine when the optimal time to conduct...merely a new lot, be thoroughly tested in the researcher's system before beginning large scale experiments...
  8. Antibodies 101: Antibody Engineering and Directed Evolution

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    Blog Post
    ...antibodies means improving research. More specific antibodies support common research tools like western blots... Antibody Topics Antibody Data Repositories and Search Engines Antibodies 101: Producing Recombinant Antibodies...
  9. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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    Blog Post
    ...efficiency. Together, these factors prevent stem cell researchers from generating clonal knock-in lines, especially... allele. For N-terminal tagging, specifically searching for error-free knock-in at both alleles may be...for Regenerative Medicine (CRM), Edinburgh. His research interests include optimizing CRISPR/Cas9 genome-editing...
  10. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

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    Blog Post
    ...processes that are crucial in the world of biological research, medical therapy, and even drug development (Grimm...stable, and versatile imaging tools in biological research. This does make finding the right dye for your...complicated. We hope this post helps you in your search for the perfect way to light up your science. Simon...
  11. Live and Let Dye: Self-Labeling Protein Tags

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    Blog Post
    ...stability, or other properties is an active area of research. If size is a concern for your application, SNAP-tag...gene of interest with these tools. Conclusion Researchers and vendors continue to develop new ligands for...Proteins Inside Living Cells. Accounts of Chemical Research, 44(9), 784–792. https://doi.org/10.1021/ar200099f...
  12. CRISPR 101: Multiplex Expression of gRNAs

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    Blog Post
    ...Cpf1.   Figure 1: Multiplexing allows researchers to express multiple gRNA from a single construct...from a single lentiviral vector.” Nucleic Acids Research 42(19) (2014): e147. PubMed PMID: 25122746. PubMed... Our Guide Pages Find CRISPR Plasmids for Your Research Browse Our CRISPR Multiplexing Resources ...
  13. Selecting Your Plasmid Purification Kit

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    Blog Post
    ...during shipping. Of the kits reviewed here, NEB’s Monarch®, QIAGEN’s QIAwave, and Milllipore Sigma’s GenElute...in plasmid purification kits Kit Series NEB Monarch QIAGEN QIAwave Millipore Sigma GenElute Reduced...recycled materials Sustainable forestry certification Starch-based compostable bags for kit components Recyclable...
  14. 2022 Depositor Week

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    Blog Post
    ...say thank you to all the many scientists and researchers who deposit with us!  This year alone, we had...Depositors Hall of Fame  Thursday: 8 Great Depositor Research Stories   Friday: Specialized Depositor Shout-...
  15. Introducing the Addgene Antibodies 101 eBook!

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    Blog Post
    ...looking to use antibody-based applications in their research.  Like all our eBooks, the Antibodies 101 eBook...For example, if you’re very new to antibodies as research tools, you may find “Chapter 1: What is an Antibody...
  16. 15 Hot Plasmids from 2017

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    Blog Post
    ...undoubtedly constitute a valuable tool for the research community. You can find the AAV vectors for infecting...imaging of two biosensors simultaneously   Researchers in the labs of Michael Lin and Ryohei Yasuda ...sodium/potassium pump ATP1A1 in addition to a researcher’s locus of interest. NHEJ- or HDR-induced mutations...
  17. Overwhelmed? Take a Break With Our 5 Favorite Science Comics

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    Blog Post
    ...evidence that laughter really can be good for you. A search on PubMed for the word “laughter” turns up a few...evidence. (What can I say, we are scientists). Researchers in Taiwan showed that a laughter program significantly...
  18. Twenty Years of Sharing Science!

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    Blog Post
    [email protected]! Fun fact! Did you know you can search for plasmids from a publication by pasting the ...the paper's title into the Addgene website search bar?   We'll be celebrating all year long, so keep an ...
  19. The Codon Guide to Synthetic Biology

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    Blog Post
    ...I spent about seven years working in research labs, and then pivoted to writing full-time in mid-2020...synthetic biology. The guide is a curated list of research papers, blogs, free courses, YouTube channels,...
Showing: 561 - 580 of 943 results