We narrowed to 710 results for: BASE;
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TypeBlog Post...Gγ). G proteins are divided into four subtypes, based on their Gα subunit: Gαs, Gαi/o, Gαq/11, and Gα12...
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Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9
TypeBlog Post...having effects on various biological disciplines, the base technology itself is constantly improving. Cas9 ... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog Post...References 1.Tang, Jonathan C.Y., et al. “A nanobody-based system using fluorescent proteins as scaffolds for... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog Post...recent models of C. elegans molecular biology are based on the standard worm genome. While C. elegans are... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog Post...search engine and sign up for Google Scholar alerts. Based on keywords that you provide, Google Scholar will... -
Caltech Systemic Capsids
TypeCollection...quality control, including AAV titering by probe-based droplet digital PCR, in vitro and (when possible... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog Post...Fluorescence fLuctuation INcrease by Contact (FLINC)-based biosensors measure fluctuations of TagRFP-T fluorescence... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog Post...The Sternberg lab in 2016 created cGAL, a GAL-4 based bipartite expression system that can regulate gene... is curated and added to other databases including wormbase and flybase. microPublication is run and maintained...pages and curated databases for scientists including Wormbook, Wormatlas and Wormbase. And scientists have...institute developed a 3’UTR database specifically for C. elegans. This database provides detailed information...coding mRNAs, and annotations extracted from other databases (Mangone et al., 2008). OpenWorm OpenWorm is ...published there as well. Worm Development Dynamics Database (WDDD) The WDDD aims to acquire quantitative data...Koji, et al. "WDDD: worm developmental dynamics database." Nucleic acids research 41.D1 (2012): D732-D737... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog Post...used to restrict expression to a single cell type based on the overlap in cell types both promoters expressed...Institute of Molecular and Clinical Ophthalmology Basel recently took a different approach to enhance specificity...targeting the same cell class in humans (R=0.67). A database of the microscopy images of all AAVs that reproducibly... -
The Materials Science of Optogenetics Experiments
TypeBlog Post...side of the optogenetics coin is materials science-based. Light is delivered to your opsin through a small... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog Post...a final list of questions for the actual event, based on these interviews. The idea is to ask questions... -
Tips for Using BLAST to Verify Plasmids
TypeBlog Post...you can extract practically every single reliable base pair from your results. This option is not as fast...sequence databases from the dropdown menu. Typically, we use the default nucleotide database “Nucleotide...plasmids with us or other entries in NCBI’s numerous databases. As our repository has grown over the years (we... explanation of the different BLAST programs, databases, and elements of the BLAST search pages. At Addgene...known reference sequence or to a BLAST sequence database. If you know the expected nucleotide sequence,...identity between your sequence and the reference/database sequence is ≥ 95%. [Our QC process would be trouble-free...blastx checks all six possible frames against the database. Another benefit is that a frameshift mutation... -
Deisseroth INTRSECT Collection
TypeCollection...virus (AAV)-borne payloads to be expressed in cells based on a doubly-specified combination of genetic and... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog Post...to help you learn more about fly biology and fly-based experimental systems. Some resources will direct...book for newbies on how to work with flies. Flybase.org is absolutely essential. You can look up information...many fly stocks that are available to everyone. Flybase lists what stocks are available at Bloomington,... -
Rabies and Neuronal Tracing
TypeBlog Post... target cell surface receptors (Callaway 2008). Based on this, cell type specificity of the starter cell... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog Post...PMID: 27381852. Choi, Kyoung-Hee, et al. "A Tn7-based broad-range bacterial cloning and expression system... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog Post...vector delivery is often used, the CRISPR approach based on HDR has the advantage of being a potentially ... -
Antibodies 101: Flow Compensation
TypeBlog Post...populations. Figure 2: Compensation example based on experimental sample. Flow plots showing a sample... -
Plasmids 101: Degron Tags
TypeBlog Post...in-frame to the protein of interest for plasmid-based expression systems. Degron tags are one of many ... -
Hot Plasmids February 2024
TypeBlog Post...neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing...