...recent models of C. elegans molecular biology are based on the standard worm genome. While C. elegans are...

...search engine and sign up for Google Scholar alerts. Based on keywords that you provide, Google Scholar will...

...used to restrict expression to a single cell type based on the overlap in cell types both promoters expressed...Institute of Molecular and Clinical Ophthalmology Basel recently took a different approach to enhance specificity...targeting the same cell class in humans (R=0.67).  A database of the microscopy images of all AAVs that reproducibly...

...Fluorescence fLuctuation INcrease by Contact (FLINC)-based biosensors measure fluctuations of TagRFP-T fluorescence...

...The Sternberg lab in 2016 created cGAL, a GAL-4 based bipartite expression system that can regulate gene... is curated and added to other databases including wormbase and flybase. microPublication is run and maintained...pages and curated databases for scientists including Wormbook, Wormatlas and Wormbase. And scientists have...institute developed a 3’UTR database specifically for C. elegans. This database provides detailed information...coding mRNAs, and annotations extracted from other databases (Mangone et al., 2008).  OpenWorm OpenWorm is ...published there as well.   Worm Development Dynamics Database (WDDD) The WDDD aims to acquire quantitative data...Koji, et al. "WDDD: worm developmental dynamics database." Nucleic acids research 41.D1 (2012): D732-D737...

...side of the optogenetics coin is materials science-based. Light is delivered to your opsin through a small...

...a final list of questions for the actual event, based on these interviews. The idea is to ask questions...

...you can extract practically every single reliable base pair from your results. This option is not as fast...sequence databases from the dropdown menu. Typically, we use the default nucleotide database “Nucleotide...plasmids with us or other entries in NCBI’s numerous databases. As our repository has grown over the years (we... explanation of the different BLAST programs, databases, and elements of the BLAST search pages. At Addgene...known reference sequence or to a BLAST sequence database. If you know the expected nucleotide sequence,...identity between your sequence and the reference/database sequence is ≥ 95%. [Our QC process would be trouble-free...blastx checks all six possible frames against the database. Another benefit is that a frameshift mutation...

...quality control, including AAV titering by probe-based droplet digital PCR, in vitro and (when possible...

... to determine the concentration of your samples based on your sample’s HC + LC area. Example calculation...

...vector delivery is often used, the CRISPR approach based on HDR has the advantage of being a potentially ...

...journal clubs have become popular, including those based on Twitter. At my former academic department, we...

...acceptor for fluorescence lifetime imaging microscopy-based Förster resonance energy transfer (FLIM-FRET) in...

... target cell surface receptors (Callaway 2008). Based on this, cell type specificity of the starter cell...

...clozapine-N-oxide, clozapine, and compound 21 in DREADD-based chemogenetics in mice. Scientific Reports 9: https...

...Antibodies Plasmids 101: Peptide Tagging Plasmid-based Recombinant Antibody Resources on Addgene.org  ...

...PMID: 27381852. Choi, Kyoung-Hee, et al. "A Tn7-based broad-range bacterial cloning and expression system...

...manager: Unlike academia, promotions in industry are based on people’s ability to manage others and work as...

...populations.   Figure 2: Compensation example based on experimental sample. Flow plots showing a sample...

...in-frame to the protein of interest for plasmid-based expression systems. Degron tags are one of many ...