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We narrowed to 700 results for: BASE;

Showing: 561 - 580 of 700 results
  1. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...you can extract practically every single reliable base pair from your results. This option is not as fast...sequence databases from the dropdown menu. Typically, we use the default nucleotide database “Nucleotide...plasmids with us or other entries in NCBI’s numerous databases. As our repository has grown over the years (we... explanation of the different BLAST programs, databases, and elements of the BLAST search pages. At Addgene...known reference sequence or to a BLAST sequence database. If you know the expected nucleotide sequence,...identity between your sequence and the reference/database sequence is ≥ 95%. [Our QC process would be trouble-free...blastx checks all six possible frames against the database. Another benefit is that a frameshift mutation...
  2. Rabies and Neuronal Tracing

    Type
    Blog Post
    ... target cell surface receptors (Callaway 2008). Based on this, cell type specificity of the starter cell...
  3. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ...to help you learn more about fly biology and fly-based experimental systems. Some resources will direct...book for newbies on how to work with flies. Flybase.org is absolutely essential. You can look up information...many fly stocks that are available to everyone. Flybase lists what stocks are available at Bloomington,...
  4. Plasmids 101: Degron Tags

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    Blog Post
    ...in-frame to the protein of interest for plasmid-based expression systems. Degron tags are one of many ...
  5. Hot Plasmids February 2024

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    Blog Post
    ...neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing...
  6. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...clozapine-N-oxide, clozapine, and compound 21 in DREADD-based chemogenetics in mice. Scientific Reports 9: https...
  7. Of Myc and Men

    Type
    Blog Post
    ...Antibodies Plasmids 101: Peptide Tagging Plasmid-based Recombinant Antibody Resources on Addgene.org  ...
  8. Academic vs. Industry Postdocs

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    Blog Post
    ...manager: Unlike academia, promotions in industry are based on people’s ability to manage others and work as...
  9. Anatomy of a Plasmid Page at Addgene

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    Blog Post
    ...the Plasmid Map: We auto-generate a plasmid map based off of the plasmid sequence and feature information... create their new plasmid.  The Addgene Vector Database has more information about common vector backbones...
Showing: 561 - 580 of 700 results