We narrowed to 958 results for: Cre

...characterized, it turns out this protein has a few more secrets - during a collaboration, members of Maureen Hanson...necessary to observe a signal. Red signal intensity increases with GFP concentration and saturates around 30...likely to be most useful for labs that already have created a transgenic GFP-containing organism,” says Hanson...

...holds yearly planning meetings with each trainee to create a supportive, growth-oriented lab culture. The ...perfectionists,” and we don’t often give ourselves credit for what we have accomplished. Starting with a ...meetings make the lab run more smoothly. With a concrete plan in hand, trainees are motivated to complete...

...that your protein might not fold well, you should screen a few different vectors to see which gives you ...subtilis and the yeast Pichia pastoris. The improved secretory capacities of these microbes make them useful ...peptide (SP) that routes the protein through the secretory pathway to improve folding and disulfide bond ...

... today. Although I wish I could take all of the credit, the success of the company is attributed to the... of an interactive game that Addgene employees created and designed for the Hall of Human Life at the ...Addgene means to them. Ken mentioned Addgene’s incredible growth and our determination to keep materials...

...Use software such as Evernote or Google Tasks to create lists of your upcoming tasks, set deadlines, and... computer or smartphone. Evernote allows you to create notes directly from webpage clippings, photographs...attachments. Organize your notes into notebooks, create checklists that link to other notes, and use the...

... interacting partners and perform whole animal screens for small molecules that block the neurodegenerative...their expression in specific cells. With a more concrete understanding of the molecular biology behind ...and pathways to target in future small molecule screens. He plans to work with a partner at IRCCS Besta...

...response to the cell cycle or other stimuli.   To create a colorful Cas9 toolbox, Ma et al. turned to SpCas9...was fused to a different fluorescent protein to create three colors. The specificity of these orthologs...nucleome a specific priority, as researchers work to increase our understanding of chromatin organization. This...

...your project's potential. Widening your search creates fertile ground for serendipitous encounters that...research and PhD studies can enrich the depth and creativity of your work, while offering mental health benefits...but also cultivate a mindset that is adaptable, creative, and poised for groundbreaking discoveries. So...

...The last trick GeoCas9 has up its sleeve is its increased longevity in human serum. Harrington et al suspected...shown that a cocaine esterase engineered to have increased thermostability also had a longer lifetime in ...Harrington, Lucas, et al. "A thermostable Cas9 with increased lifetime in human plasma." bioRxiv (2017): 138867...

...plasmid analysis tool including editing, annotating, creating maps, and more. This tool is maintained by M. ...University of Utah.   Figure 1: A plasmid map created in SnapGene of Blue Flame plasmid pET21a-BirA. ...repository for Cas enzymes.  Open repository of CRISPR screens Cellosaurus: A knowledge resource for cell lines...

... the connection between LEGOs and science more concrete to help further unlock the potential contained...process One major challenge when we started was creating a LEGO stage that resembles a real laboratory.... your experiments to think outside the box, be creative, and put your inner child to work. If you have...

...provide advice. The society organizes numerous recreational and educational events throughout the year, ...iGEM alumni-industry networking to promote the creation of start ups and biotech spin offs. The Synthetic...Inspiring future scientists At Addgene, we’re incredibly glad to see these biological societies engaging...

...Was I jaded by my time in academia? Did I run screaming from the lab like the cartoon on the right?!? ...’t have to mean abandoning it or running away screaming, but instead can involve walking alongside it ...it and giving it a high five. While screaming can be therapeutic and I’ve certainly done my fair share ...

... these meetings in relatively isolated resorts creates a sense of camaraderie with fellow attendees from...depositor, discussed some of the many challenges of creating biological circuits, and I can only say this science...opportunity to share their cutting edge science in increasingly important field.  Interested in Addgene's ...

...single-strand DNA incorporation (ssDI). "Easi-CRISPR for creating knock-in and conditional knockout mouse models...Protoc. 13(1) (2018):195-215. PubMed PMID: 29266098 Creating knock-in mouse models with CRISPR has been challenging...efficiency. Easi-CRISPR uses a long ssDNA template to increase efficiency of insertion and gene replacement. ...

...we found that adding a TEV linker dramatically increased accessibility of the AviTag and HisTag, which ...fusion protein in bacteria. Our initial attempt to create a plasmid based on the biotin ligase BirA backbone... inserting BirA into a pMal plasmid (NEB) thus creating the pMal-T-Avi-His/BirA plasmid now available ...

...primary antibody and endogenous IgGs in the tissue. Created with biorender.com. Strategies for avoiding...Some labs have found that you can significantly decrease background staining by mixing your primary and...you add your primary and secondary antibodies. Created with biorender.com. Proper tissue processing...

...Recombinant antibodies are plasmid-based and are created synthetically or derived from hybridoma antibody...antibodies against the new Fc region. In addition to increasing multiplexing capabilities this process is frequently...Although isotype conversion is routinely used to increase the utility of recombinant antibody tools, the...

...the community, increasing the impact of the work, and as a collateral benefit, increasing the impact of...Tompson Institute.  References Baldwin, Melinda. "Credibility, peer review, and Nature, 1945–1990." Notes and...

...individual cells. Each cell is labeled with 3-4 tags to increase the diversity of the CellTag library. The fixed...detected over a 10-week period. Their expression decreased over time but they were not silenced.    Find ...retroviral overexpression cocktail yielded a three-fold increase of fully reprogrammed cells based on single-cell...