We narrowed to 753 results for: ELP
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TypeBlog Post... R1 institutions to community colleges, and has helped provide original research experiences for thousands...
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CRISPR 101: Making a Knock-In Cell Line
TypeBlog Post...about Addgene’s knock-in plasmids for reagents to help you knock your knock-in experiment out of the park... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog Post...to be aware of and actively use tools designed to help reduce or eliminate bias, just like the ones made... -
CRISPR 101: RNA Editing with Cas13
TypeBlog Post...in the guide across from these off-target edits helped to minimize local off-target editing by RESCUE.... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog Post...Fabienne Furt, and Quintin Marcelino for their helpful discussion of this book.... -
Simplify Cloning with in vivo Assembly
TypeBlog Post...sequence manager, such as SnapGene, can be very helpful to confirm primer design. Use minimal template... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog Post...approaches you with glowing feedback from a client you helped. Maybe you awkwardly say thanks and move on with... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog Post...Brett Mensh, a wordsmith soldier of fortune, for helpful comments. Many thanks to our guest blogger, Luke... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog Post...between labs, initial titration experiments might be helpful to achieve optimal results. It is important to ... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog Post...Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells, which are difficult... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog Post...constructs like plasmids often include GFP as a marker to help identify which cells have successfully taken up ... -
CRISPR 101: Any Base Transversion Editors
TypeBlog Post...AAG to improve editing efficiency, but it didn’t help: it seemed that the endogenous enzyme is not efficient... -
Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog Post...target is not expressed. The negative control will help you determine if there is non-specific binding or... -
Viral Production at Addgene
TypeBlog Post...serotype-specific cap genes, and a plasmid encoding adenoviral helper sequences. AAV preparations are purified by iodixanol... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog Post...have any questions, don’t hesitate to email us at help@addgene.org. Happy screening! This blog was written... -
Antibodies 101: Immunoprecipitation
TypeBlog Post...overview of the IP method. If you found this blog helpful be sure to check out Addgene’s blog for other antibody... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog Post...problematic, however. Well designed aptamers can help reduce such background, thereby improving traditional... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog Post...option in such instances is palliative care, which helps patients manage the pain and discomfort caused by... -
Hot Plasmids - August 2020
TypeBlog Post...piggyBac vector integrates into genomic DNA with the help of the piggyBa transposase. Image from Hazelbaker... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog Post...created an array of base editing reporters that can help you do just that. Monitoring APOBEC and Cas9-mediated...