We narrowed to 756 results for: ELP

1. CRISPR 101: RNA Editing with Cas13

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   Blog Post

   ...in the guide across from these off-target edits helped to minimize local off-target editing by RESCUE....

2. Capturing a Moment with Marc Zimmer’s “The State of Science”

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   ...Fabienne Furt, and Quintin Marcelino for their helpful discussion of this book....

3. Transferable Skills Guide: Identifying Your Transferable Skills

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   ...approaches you with glowing feedback from a client you helped. Maybe you awkwardly say thanks and move on with...

4. Uncovering Ancestral Bias in CRISPR Research

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   ...to be aware of and actively use tools designed to help reduce or eliminate bias, just like the ones made...

5. An Introduction to Adenovirus

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   ... deleted. These genes’ functions are rescued by helper plasmids used during production and/or by genes...

6. Viral Vectors 101: The Retroviral Lifecycle

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   ... and of the normal, replicative life cycle — can help you understand these seemingly odd choices and lay...

7. Course-Based Undergraduate Research Experiences (CUREs)

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   ... R1 institutions to community colleges, and has helped provide original research experiences for thousands...

8. Simplify Cloning with in vivo Assembly

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   ...sequence manager, such as SnapGene, can be very helpful to confirm primer design.  Use minimal template...

9. CRISPR 101: Making a Knock-In Cell Line

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   ...about Addgene’s knock-in plasmids for reagents to help you knock your knock-in experiment out of the park...

10. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    Blog Post

    ...Brett Mensh, a wordsmith soldier of fortune, for helpful comments. Many thanks to our guest blogger, Luke...

11. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    ...between labs, initial titration experiments might be helpful to achieve optimal results. It is important to ...

12. Cpf1: A New Tool for CRISPR Genome Editing

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    Blog Post

    ...Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells, which are difficult...

13. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post

    ...constructs like plasmids often include GFP as a marker to help identify which cells have successfully taken up ...

14. CRISPR 101: Any Base Transversion Editors

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    ...AAG to improve editing efficiency, but it didn’t help: it seemed that the endogenous enzyme is not efficient...

15. Antibodies 101: The Four ELISAs and When to Use Them

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    ...target is not expressed. The negative control will help you determine if there is non-specific binding or...

16. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    ...models, tissue banks, Rett databases, and other helpful tools.  References: Amir RE, Van den Veyver IB...

17. To Codon Optimize or Not: That is the Question

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    Blog Post

    ...such as IDT, Genewiz and GenScript, offer tools to help with these decisions.  So when do you want to codon...

18. Hot Plasmids - August 2020

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    ...piggyBac vector integrates into genomic DNA with the help of the piggyBa transposase. Image from Hazelbaker...

19. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    ...created an array of base editing reporters that can help you do just that. Monitoring APOBEC and Cas9-mediated...

20. Plasmids 101: Plasmid Incompatibility

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    ...Shintani et al., 2015). Now, bioinformatics tools help researchers determine how compatible their plasmids...