We narrowed to 621 results for: Emb

1. CRISPR 101: Making a Knock-In Cell Line

   Type

   Blog Post

   ...out your Cas9? It can be! Cas12a is a Cas family member which generates sticky ends upon cutting. Cas12a...

2. Cpf1: A New Tool for CRISPR Genome Editing

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   Blog Post

   ...mammalian cells. Like Cas9 nucleases, Cpf1 family members contain a RuvC-like endonuclease domain, but they...

3. Plasmids 101: Plasmid Incompatibility

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   ...resistant plasmids from Enterobacteriaceae family members within the mouse gut (Kamruzzaman et al., 2017)...

4. New CRISPR Tools: Cas7-11 and PASTE

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   ...sensitive to toxicity from RNA editing, such as mouse embryonic stem cells and glioblastoma cells. PASTE Development...

5. Antibodies 101: Immunoprecipitation

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   ...adjusted depending on the sample. It is important to remember that sample lysates will also contain proteases...

6. Viral Vectors 101: Pseudotyping

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   ...find tables and information on different viral membranes, tropism, and advantages and disadvantages: Table...

7. Deciphering the Mysteries of Behavior with Viral Vectors

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   Blog Post

   ...noradrenergic excitability upon acute stress." The EMBO journal 37.21 (2018). PubMed PMID: 30209240. PubMed...

8. Save Time with Transient Plant Leaf Transformations

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   Blog Post

   ...working vector system This allows you to quickly assemble vectors and test different ideas. We are a huge...

9. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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   Blog Post

   ...identified. While LY6A or other Ly6 gene family members are linked to viral susceptibility (mouse adenovirus...

10. Neuronal labeling with Spaghetti Monster

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    Blog Post

    ... Flying Spaghetti Monster; the smFP structure resembles images of the deity (see comparison image above...

11. Transferable Skills Guide: External Collaborations

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    ...feeling weird and started to be fun. I just had to remember to approach the relationship with the perspective...

12. Viral Production at Addgene

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    Blog Post

    ...filtration through a 0.45 µm polyethersulfone (PES) membrane. The preparations are then aliquoted, frozen, ...

13. Hot Plasmids Spring 2024

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    Blog Post

    ...nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane protein (Plasmid #141386-LV [+M], negative control...

14. AAV Titers: Where Do They Come From And What Do They Mean?

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    Blog Post

    ...Remember the game show “The $25,000 Pyramid” where one player tries to get the other to guess a category...

15. Transferable Skills Guide: Career Planning Resources

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    Blog Post

    ...clearly a good idea to be diligent in your research, remember that you will not be a graduate student or postdoc...

16. Career Insights: Technical Support Specialist

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    ...the larger biotech companies), and as far as I remember, it was the first time that I was absolutely convinced...

17. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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    Blog Post

    ...al. 2016. “Inhibitory Synapses Are Repeatedly Assembled and Removed at Persistent Sites In Vivo.” Neuron...

18. Healthcare Consulting: A Door to the Business of Life Sciences

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    Blog Post

    ... on the expertise of the senior leadership and members of their consulting staff. Who would enjoy healthcare...

19. Getting the Most from Your Lentiviral Transduction

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    Blog Post

    ...., Rubinstein, M. “LDL receptor and its family members serve as the cellular receptors for vesicular stomatitis...

20. Plasmids 101: Control Plasmids

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    ...difference and is it significant? Vaux DL, et al. EMBO Rep. Apr; 13(4): 291–296. (2012). PubMed Central...