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We narrowed to 1,145 results for: Gene

Showing: 561 - 580 of 1145 results

  1. Typing CRISPR Systems

    Type
    Blog Post
    ...domain due to a gene that resembles cas10. All type IV subtypes are missing adaptation genes, Cas1 and Cas2...sequence similarity, phylogenetic analysis, neighborhood analysis of surrounding genes, and experimental ...straightforward as it seems. There is not a single gene that is shared across all CRISPR types; the fast...together because they contain a distinct Cas7-type gene. The exact mechanism of type IV systems is not known... and applications with the type I CRISPR system. Gene and Genome Editing, 3–4, 100013. https://doi.org...the array of CRISPR technologies. Learning the general principles of this classification system will help...A–C, with the beloved SpCas9 (from Streptococcus pyogenes) belonging in II-A. Cas9 was the first to be adapted...

  2. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...’s first genetic engineering company, Genentech, was founded.  This commercialization of gene editing ...before the discovery of genes themselves.  How has scientific progress in genetic editing impacted the world... DNA sequences is not ideal. For the early generation gene editors, new amino acid recognition sites had...followed by non-homologous end-joining that results in gene knockouts.   Star Trek: Into Darkness (2013) which...The major drawback of all the endonuclease-based gene editors (meganucleases, ZFNs, TALENs, and CRISPR-Cas...Gregor Mendel on the genetic inheritance (1866) and just when the Mendelian genetics was being revived by...Zinc-Finger Nucleases. Genetics 188:773–782 . https://doi.org/10.1534/genetics.111.131433 Doudna JA, Charpentier...

  3. iPSC Neurodegenerative Disease Initiative Plasmid Collection

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    Collection
    ...used to create cell lines with endogenously-tagged gene variants or to differentiate human iPSCs into other...Find plasmids generated by the iPSC Neurodegenerative Disease Initiative (iNDI) here. Depositor...Stem Cell Research Neurodegeneration Research Collection The iPSC Neurodegenerative Disease Initiative...plasmid collection at Addgene. Figure 1: Schematic of iNDI iPSC line generation, phenotyping, and distribution...Collections iNDI iPSC Neurodegenerative Disease Initiative (iNDI) Collection You May Also Like... CRISPR ...Initiative (iNDI) project aims to generate and characterize single nucleotide variant lines in an isogenic cellular...NIH. Reference Ramos et al. 2021. Tackling neurodegenerative diseases with genomic engineering: A new stem...

  4. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...tags for you to create fusion proteins with your gene of interest. Please visit Michael Davidson's Molecular...Empty Backbones FRET Subcellular localization Optogenetics The lab of Michael Davidson from Florida State...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  5. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    ...URL “http://n2t.net/addgene:87155” where the Addgene registered prefix and the Addgene ID # are directly...Daniel Bauer (Addgene plasmid # 87155 ; http://n2t.net/addgene:87155 ; RRID:Addgene_87155).” So when citing...Daniel Bauer (Addgene plasmid # 87155 ; http://n2t.net/addgene:87155 ; RRID:Addgene_87155)” Cite plasmid...through Addgene and find verified information about each plasmid item. Given a specific Addgene ID # listed...available through Addgene. Let’s take a look at lenti-Cas9-VQR-Blast, which has the Addgene ID #87155. The... as “RRID:Addgene_87155” and the following URL (https://scicrunch.org/resolver/RRID:Addgene_87155) will...contain the Addgene ID # and the information necessary to indicate that this ID is managed by Addgene. How to...

  6. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ... maintain the genes they carry among organisms belonging to many different phylogenetic groups (Jain and...that the plasmid is conjugative and carries the genes necessary for transfer initiation between bacterial...plasmids for DNA cloning in Gram-negative bacteria. Gene, 70(1), 191–197. https://doi.org/10.1016/0378-1119... S. (2011). Broad-host-range plasmid vectors for gene expression in bacteria. Methods in Molecular Biology...viscosus, Aerobacter aerogenes, Aeromonas hydrophila, Agrobacterium tumefaciens, Alcaligenes eutrophus, Azotobacter...salmonicida, Agrobacterium tumefaciens, A. rhizogenes, Alcaligenes eutrophus, Enterobacter sp., Erwinia amylovora... broad host-range plasmid RK2. Molecular & General Genetics: MGG, 152(3), 129–135. https://doi.org/10.1007...

  7. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...when trying to edit a gene using homology directed repair, since HDR-mediated gene editing is most efficient...formerly Cpf1), a nuclease that generates double strand breaks in target genes resulting in the formation ...always positioned correctly to target a particular gene. Furthermore, a target sequence may have high homology...plasmids from this publication at Addgene. Additional Resources on the Addgene Blog  Read more about Cpf1...sequences While PAM sequences for the commonly used S. pyogenes Cas9 (3'-NGG) are abundant throughout the human...circumvent this limitation: 1) the use of novel S. pyogenes Cas9 variants with varying PAM sequences, 2) the...Cas9 homologs derived from species other than S. pyogenes, and 3) the use of non-Cas9 enzymes. (For more...

  8. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ...edits of interest than solely transfected cells. “Gene On” (GO)- a functional reporter system to identify... (Katti et al., 2020). They named this reporter “Gene On” or GO. GO works by affecting protein translation...fluorescent proteins. Figure 4: Reporters in Gene On include a mScarlet reporter, luciferase reporter...and enrich for base-editing events in vivo. TLDR: Gene On (GO) from the Dow lab is a flexible reporter ...base-pair mCherry insertion. Editing of these motifs generates lesions that are subject to excision and ssDNA...This edit changes the histidine to a tyrosine generating a GFP variant (GFPY66) that has a shifted emission...hypothesis with GFP fluorescence. To do this they generated a silent GFP construct, that contains an ACG start...

  9. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    ...homology arm as a gBlocks® Gene Fragment from Integrated DNA technologies. Generally speaking, anyone using... an effort to track down every last one of those gene regulatory proteins and the places in the genome...together on several projects and this is one related to gene expression. One goal of the ENCODE project is to...with detailed protocols, go to www.addgene.org/crispr/tagging. Addgene: What motivated you to adapt CRISPR...tagging system, which they’ve now made available at Addgene. For more details on the plasmids that are currently...can now look at many more transcription factors. Addgene: How does the system work? Mendenhall: The system...antibodies. The results look almost identical. Addgene: What advantages does your system have in this ...

  10. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    ... to turn on genes at different times. It requires more complex synthetic networks. Addgene: What hurdles...on an engineering project including hundreds of genes and regulation and resource control - everything...gives us enough hardware to control hundreds of genes. We’ve created enough regulatory elements, CRISPRs...the scale of genetic engineering, with the ultimate objective of genome design.” It’s genetic engineering...larger genetic circuits that could conceivably be as large as natural regulatory networks. Addgene: What...things are easy with CRISPR. Addgene: You’ve made many deposits to Addgene over the years. Can you describe...toolbox for taking on very large genetic engineering projects. Addgene: How easy is it to do this now? ...

  11. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...the complexity of genetic circuits, allowing for tighter spatio-temporal control of gene expression within...world of possibilities in the engineering of complex gene networks. A comparable system for targeting specific...will make those new plasmids available through Addgene, and they hope others will do the same. “This isn...10.1186/1754-1611-8-7. PubMed.  Related Resources Addgene's Synthetic Biology Overview More Synbio Blog Posts...

  12. Depositor Collections

    Type
    Collection
    ... pLEG/pREG Modular Viral Vector System - Dankort Gene Vector Core Viral Vectors - Oka...Depositor Plasmid Collections Depositor Collections Addgene's repository contains 161,873 plasmids, which includes...Michael J. Fox Foundation Plasmid Resource iPSC Neurodegenerative Disease Initiative (iNDI) Collection Rett ...expression of recombinant phosphoproteins - Rinehart FreeGenes Project Open Enzyme Collection Screening Multiplexed...

  13. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...in neurons. (b) Genetic cargo can be customized to control transgene expression. Gene regulatory elements....     Figure 1: AAV toolkit for targeted gene delivery to the brain. The AAV (a) capsid, (b) cargo...Abbreviations: AAV, adeno-associated virus; GRE, gene regulatory element; NHP, non-human primate. ...LY6C1, with relatively even distribution of target gene expression (EGFP, in the depositing manuscript) ...Systemic AAV vectors for widespread and targeted gene delivery in rodents. Nature Protocols;14, 379–414...2017). Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous systems...Gradinaru, V. (2022). Engineered AAVs for non-invasive gene delivery to rodent and non-human primate nervous...

  14. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...looks like the genetic cargo in one plasmid (the transfer plasmid), the envelope (Env) gene on a separate...with those particles. Since the Env, Gag, and Pol genes are not encoded in the transfer plasmid, the component...Similarly, if the viral vector carries an shRNA to a gene essential for cellular function, this would also...Resources Resources on Addgene.org Addgene viral services Addgene Biosafety Guide Addgene Viral Plasmids ...? That’s okay! In this article we will discuss general viral safety considerations and best practices ...of what is being integrated by the virus. If an oncogene overexpression construct is being delivered, that... and the same holds true for types of viruses generated in the lab. The four most common types of viruses...

  15. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...Additional Resources on the Addgene Blog Treating Muscular Dystrophy with CRISPR Gene Editing Learn to Use ...Cas9 have also been used to create mutations in genes essential to HIV-1 replication. However, this approach...modification targeting either the LTRs or essential genes. Many of these escape mutations were located near...Genomes from Human T-lymphoid Cells by CRISPR/Cas9 Gene Editing. Sci Rep. 2016 Mar 4;6:22555. PubMed PMID...Screens Additional Resources onf Addgene.org Check out Addgene’s newly updated CRISPR Guide! Find CRISPR...Activation Mediators (SAM) system available from Addgene. This system uses the dCas9-VP64 fusion commonly...others and accelerate the pace of research. We at Addgene hope to see the problem of in vivo CRISPR delivery...

  16. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...new green glowing protein could be used to measure gene transcription, as well as to track protein localization... he was right! He began to study the A. victoria gene responsible for encoding GFP, and, in 1992, he reported...

  17. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type
    Blog Post
    ...Additional Resources from Addgene Learn about Retinal Gene Therapy from Connie Cepko Find Plasmids from the...treat disease through tissue repair, stem cells, gene therapy, and tissue engineering. How the multidisciplinary...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Darrell Kotton is the director of the Center for Regenerative Medicine at Boston University Medical School...source biology in his own lab, at the Center for Regenerative Medicine, and within the biomedical research...Along these lines, Darrell recently joined the Addgene Board of Directors where he hopes to help keep ... Kotton and the Boston University Center for Regenerative Medicine. Darrell describes his career goals...

  18. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...ultrasound to image bacteria in vivo: acoustic reporter genes’ on our blog. Creative approaches to presenting...Bourdeau, Raymond W., et al. "Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian...way to visualize them. You can also “hear” them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented...loads of inspiration for the current and the next generation of microbiologists. If you got inspired to produce...PubMed Central PMCID: PMC5920530.  Resources on the Addgene Blog Read Our Post on CRISPR Methods for Bacterial...Science Communication using Twitter  Resources on Addgene.org Check out Our Microbiology Resource Page CRISPR...bacteria Deposit Your Microbiology Plasmids with Addgene ...

  19. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...editing in mammalian systems. Mammalian ID Plasmid Gene/Insert PI Publication Do you have suggestions for...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  20. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...overexpressing a gene or protein after transfection). Future of FRET The first genetically encoded FRET biosensor...Resource page and can be used to create fusions with a gene of interest. These plasmids will be most useful ...designed to measure specific small biomolecules or gene activity are often intramolecular probes, as the...name a few.   Find Many FRET-Based Biosensors on Addgene's Biosensor Pages   Recent innovations have demonstrated...PubMed PMID: 26210398. Additional Resources on the Addgene Blog Tips for using FRET in Your Experiments Learn...FRET Based Biosensors Additional Resources on Addgene.org Find FRET Pairs Check Out Fluorescent Protein...
Showing: 561 - 580 of 1145 results