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We narrowed to 1,020 results for: LEA

Showing: 561 - 580 of 1020 results

  1. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    ...and longer genome search times. Using a machine learning approach, the lab proposed multiple sets of Cas9...editing of A•T to G•C in genomic DNA without DNA cleavage. Nature, 551(7681), 464–471. https://doi.org/10.1038...base in genomic DNA without double-stranded DNA cleavage. Nature, 533(7603), 420–424. https://doi.org/10.1038... variants via scalable engineering and machine learning. Nature, 643(8071), 539–550. https://doi.org/10.1038...

  2. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...intuitive, qualitative interpretation of this gel is misleading. We can see why on the S-shaped curve. On the...on the band intensity axis. This effect causes misleading qualitative conclusions, and it’s also problematic...approaches Cartoons are nice and all, but we’ll leave you with this real-world comparison of different...of western blot normalization. May your blots be clear and your detection range be linear! Resources and...

  3. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...degraded by uracil DNA glycosylase (UDG) and Exonuclease III (ExoIII) (step 4). UDG recognizes uracils...from newly synthesized DNA. Lack of both enzymes leads to a buildup of uracil and the insertion of uracil...limitation is sequencing depth and cost. You need at least 100-200 reads per variant, so the more codons you...to Generate Specific Mutations in Your Plasmids Learn How You Can Use CRISPR Pooled Libraries to Generate...

  4. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...problems. This can lead to a lack of trust in the mentor and this training is clearly not equitable to ...assess the current state of research became more clear throughout the various sessions of the symposium...first couple of sessions of the symposium it was clear that, even though postdoc associations across different...

  5. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...strain-specific tropism.” For example, AAV-PHP.B doesn’t lead to enhanced transgene expression, compared to AAV9...mice use LY6A to cross the blood-brain barrier, leading to strain-specific tropism (See S3 table (Huang... can visit our Caltech Systemic Capsids page to learn more about that collection. Table 1: Systemic capsid...systemic AAV experiments and Addgene’s AAV Data Hub to learn more about the capsid you’re interested in. Then...Fish, K. N., & Pfenning, A. R. (2022). Machine learning sequence prioritization for cell type-specific...Troiano, E. C., Moghadaszadeh, B., Peacker, B. L., Leacock, K. A., Horwitz, N., Beggs, A. H., Wagers, A. J...

  6. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...expression, but causality and mechanisms remain unclear. Today, targeted epigenetic modification at specific...came epigenome-engineering tools - zinc finger nucleases and TALENs fused to epigenetic modifiers enabled...Christopher Newgard’s Lab has fused LSD1 to the smaller nuclease-dead Staphylococcus aureus Cas9 (Sa-Cas) and deposited...comments! Find Plasmids for Epigenetic Modification   Leah Schwiesow contributed to updating this article. ...

  7. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...trafficking and their detection can suffer from photobleaching and cellular autofluorescence (Tsien, 1998)...plates or media, which can obscure weak signals; photobleaching from the excitation light, causing the signal...excitation generating reactive oxygen species that can lead to phototoxicity, perturb biological processes, ... functions as an ion channel, mutations of which lead to cystic fibrosis, one of the most prevalent inherited...

  8. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...multimer present in your sample. In these instances, please note that the supercoiled dimer band can sometimes... presence of a multimer in your sample remains unclear after visualizing it on a gel, one of the other...consistent with the size of a supercoiled plasmid dimer. Please note that it is common to see other plasmid confirmations...Ausubel FM. Recombination between bacterial plasmids leading to the formation of plasmid multimers. Cell. 1976...

  9. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...reducing agent, typically containing thiols, to cleave disulfide bonds. Load the samples, one sample per...conditions from those used by the vendor then it may lead to nonspecific binding.  Whenever possible, include...in making your own knockout line for validation, please view this tutorial from Stuart Orkin's and Daniel...protein causes a thicker band, while less protein leads to a thinner band. It is important to note, however...

  10. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ... and the two HindIII digested ends will anneal leaving the 5’ end of your gene just downstream of the ...require you to visualize your DNA using UV light – please note that UV light can damage DNA and that proper... colonies on the backbone + insert plate (or at least more colonies than the backbone alone plate). Unsuccessful...Blog  Additional Resources on the Addgene Blog: Learn How to Verify Your Plasmid Try Out Golden Gate or...

  11. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...Want to learn more about genome editing with CRISPRs? Check-out Addgene's CRISPR Resources Learn about...selective cassette minimizes the sequence added, leaving a 34 bp “scar”, but currently requires additional... editing and conditionally manipulate proteins. Learn more at his webpage or follow him on Twitter.   ...

  12. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    ...prevents both the endogenous RecBCD and SbcCD nucleases from digesting linear DNA introduced into the ...E. coli. Exo: Exo is a 5’→3’ dsDNA-dependent exonuclease.  Exo will degrade linear dsDNA starting from...with these promoters (lacI, araC, cI857) to limit leaky expression of the Red system.  Using a plasmid to...the red genes needed for recombineering, but also leads to expression of the int and xis genes which are...

  13. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...epifluorescence microscopy wasn’t able to give a clear view of cellular movement in real time. Two-photon...microscopy has uncovered host-pathogen interactions leading to the understanding of effector function in infected...Weninger. "Dendritic cell behaviour in vivo: lessons learned from intravital two-photon microscopy." Immunology... Get Tips on Choosing Your Fluorescent Protein Learn Some of the Pitfalls of Working With Fluorescent...

  14. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...that introducing a Leu28Cys mutation in eDHFR does lead to covalent binding of the TMP-tag ligand (Gallagher...a different event where CP appears briefly then leaves. Images reused from (Ulrichs et al., 2023) under... if you are comfortable with it or can find a colleague to help you, synthesizing your own ligands can... D., Karassina, N., Zimprich, C., Wood, M. G., Learish, R., Ohana, R. F., Urh, M., Simpson, D., Mendez...

  15. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...such as fluorescent microscopy must be used. To learn more about the most suitable fluorescent microscopy...structure of the protein. In some cases this can lead to changes or a complete loss of protein function...more autofluorescence and nonspecific staining leading to false positives. Numerous dyes can be used for...Check out other methods using fluorescent proteins Learn more about antibodies Resources on Addgene.org ...

  16. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    ... the pPSU2 plasmids are available from Addgene. Learn How to Analyze a Restriction Digest References ...Baby Gel Box Find 5 Smart Phone Apps for Lab Life Learn About the Evolution of Lab Techniques Resources...Kit-Free Alkaline Lysis Plasmid Miniprep Protocol Learn How to Analyze Your Restriction Digests Make Your...

  17. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    ... company, Science with Tom. In this podcast, we learn more about Tom and pick his brain for advice on ...importance in understanding biological history. 4. Please Don't Kill My Hive Beautifully filmed video about...generation science standards and communicate science. Learn more about Tom on his youtube channel and at sciencewithtom.com...

  18. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    ...forward with these efforts, you’ll find new ways to learn about science careers, lab protocols, and, of course...read through each one, plug in some headphones to learn more about the latest and greatest plasmids through...Plasmid webpage. We hope you enjoy this new way to learn about plasmid technologies. If you have any comments...

  19. Our New Antibody Mascot is....

    Type
    Blog Post
    ...journey is at an end! Their name is...(drum roll, please!)... Abi! Thank you so much to everyone who submitted...Which name best fits our new immunoglobulin friend? Please help us decide by voting for your favorite on this...Original Blugene and Aavery would like your attention, please, as they introduce their new friend…  Fig...

  20. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    ...Ready-to-Use CRISPR Lentivirus Learn about Other Members of the Addgene Leadership Team ....     Additional Resources on the Addgene Blog Learn More About Alex's Work with Cas9 Activators Michael...
Showing: 561 - 580 of 1020 results