...used CRISPR/Cas9 extensively for the purpose of making sequence-specific changes in human induced pluripotent...alleles had unwanted insertions/deletions, therefore making those alleles useless for our studies. If one now...

...easy, but the risk of PCR-introduced mutations can make alternative approaches more favorable if you want...restriction sites. Alternatively, if you want to make your protein non-functional, you may simply introduce...

... unmethylated DNA, and more. Many mutations that make these features possible are present in most commercial...commercial strains, especially mutations that make major improvements such as those that increase plasmid...

...exquisite for the so-called base editor Cas9, which makes DNA changes in the absence of dsDNA breaks (Rees... modification of interest, it would be unwise to make conclusions on the basis of the activity of a single...

... disease states, and targeting the epigenome may make certain processes, like cellular reprogramming of... likely cell type- and context-dependent, it may make sense to try multiple CRISPR tools when setting ...

...plasmids encoding antibodies from Addgene that you can make on your own.  To narrow your search, first pay attention...knocked down or knocked out. If you are interested in making your own knockout line for validation, please view...

...scenarios below for a CRISPR experiment which involves making specific knock-out edits in a gene(s) of interest...off-target frequency of 5%. Your experiment involves making a large sgRNA library to thousands of genes and...

...recombination-mediated genetic engineering.  It can be used to make an assortment of modifications: insertion and deletion...with 4-5 silent changes in the wobble codons, i.e. make changes to the third base pair of the adjacent 4...

...the parental animal, are rescued progeny, which makes screening extremely rapid. This stringent selection...knock-ins were occurring 24 hours post-injection, making screening for knock-ins much more efficient. Pure...

...switch from a dark state to a red fluorescent state make them useful for 2-color imaging with green PA-FPs...fluorescence usually means more photons are being emitted, making it easier to capture a clear image above background...

...during chemical synthesis of these molecules. To make large amounts of pure product in an affordable way...constitutive and inducible promoters, linkers, and tags makes EcoFlex a great option for those interested in recombinant...

...immunoglobulin (Ig) heavy chains and two Ig light chains make up each antibody molecule. The Fc region and part...epitopes on the protein. However, each animal will make antibodies against different protein epitopes and...

...controls needed to pressure-test your FRET assay to make sure it is working? This article addresses these...FRET experiments. However, remember that, if you make an FP fusion, you are inserting about 20-25 kDa ...

...ability to leverage the prestige of the employer makes EB-1Bs a very attractive option.    Your Expert ...a career search. Doing these simple things will make it easier for you to find and organize all of the...

... plateau stage after exponential amplification, making it difficult to properly analyze your data.  To...understanding how the analyses work - you do need to make sure the assumptions in your dataset match the assumptions...

... no fear - plasmids from Addgene depositors are making it easier than ever to use CRISPR  in E. coli and...the solution to this inefficiency? Use CRISPR to make it a selectable process! As NHEJ is ineffective ...

...The ease and flexibility of the LACE technology makes this system widely accessible for many potential...interest within the INT location. One plasmid to make note of is the INT construct bearing the "Bunch ...previous studies, and provide new insights into the make-up of multiprotein complexes like the EPC1 complex...

... While helpful from a biological standpoint, it makes delivering AAVs to the brain difficult. Traditionally... References Asencor, A. I., Dvoryanchikov, G., Makhoul, V., Tsoulfas, P., & Chaudhari, N. (2022). Selectively...

...vectors for many experimental purposes. To help you make sense of all the viral vector information that's...

... on the Addgene blog Learn more about Blugene's make-over Get a how-to guide on the new Addgene search...