We narrowed to 48 results for: Mak

...Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J,...sequences. (1) The CRISPR array is transcribed to make the pre-CRISPR RNA (pre-crRNA). (2) The pre-crRNA...PAM sequences and number/types of Cas proteins. Makarova et al. ’s classification defines 5 types and 16...ability to precisely target CRISPR to a given locus makes it especially amenable to genetic screens. Pooled...targeting in AT-rich genomes. Cpf1's small size also makes it suitable for multiplexing and use in AAV. Scientists...a specific flanking sequence on the target RNA, making it a very flexible system. Other Applications Scientists...have also used the targeting capability of Cas9 to make other types of modifications at specific loci. Activate...

...Flp AND Vcre No References Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee SY, He M, Tucciarone J, Selimbeyoglu...Hafner G, Witte M, Guy J, Subhashini N, Fenno LE, Ramakrishnan C, Kim YS, Deisseroth K, Callaway EM, Oberhuber...Poulin JF, Caronia G, Hofer C, Cui Q, Helm B, Ramakrishnan C, Chan CS, Dombeck DA, Deisseroth K, Awatramani...Limoges A, Brockway E, Müller K, Fenno L, Kim YS, Ramakrishnan C, Andrási T, Deisseroth K, Holmes A, Hájos ...new window) Yu K, Ahrens S, Zhang X, Schiff H, Ramakrishnan C, Fenno L, Deisseroth K, Zhao F, Luo MH, Gong... M, Johansson Y, Fuzik J, Fürth D, Fenno LE, Ramakrishnan C, Silberberg G, Deisseroth K, Carlén M, Meletis...Burnham N, Cristiano C, Dorrier CE, Tipton GJ, Ramakrishnan C, Kozicz T, Deisseroth K, Thiele TE, McElligott...

...generating gRNAs makes CRISPR one of the most scalable genome editing technologies, making CRISPR perfect... Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. ...very close proximity to the region to be edited, making the PAM sequence (NGG) limiting. This PAM is abundant...Anti-CRISPR Small Precision Edits CRISPR can be used to make small, very precise edits of a handful of bases,...RNA-binding protein to stabilize pegRNAs Twin PE — two PEs make complementary edits to opposite strands Prime editing...the ability to target almost any genomic locus, makes CRISPR an ideal genome engineering system for large-scale...large-scale, forward genetic screening. CRISPR can make highly specific, permanent genetic modifications...

...is the rarest codon in Escherichia coli , which makes it the most commonly used choice. Other options,...ncAA) in a protein of interest. Applications By making small changes in selected amino acids within a ...working off of a previously established protocol, make sure to match the growth medium, ncAA concentration...orthogonal tRNA. Therefore many controls must be used to make sure that these conditions are true. Always express...protein with and without the ncAA in the media to make sure that the full length protein is only made when...and References GCE4All Research Center GCE4All: Making Genetic Code Expansion Accessible Using Phosphoserine...

... CRISPR/Cas Toolkit Kanemaki Lab Auxin-Inducible Degron Tagging Masato Kanemaki's lab has developed a ...alternative DSB repair pathway, instead of HDR, which makes it easy to construct a donor vector, because MMEJ...

...and improving the orthogonal translation system to make phosphorylated proteins with one or more phosphorylation...expressing recombinant phosphoproteins. These attributes make rEcoli XpS (Bacterial Strain #192872) and pSerOTS-C1...Study Protein Phosphorylation Read about GCE4All: Making Genetic Code Expansion Accessible...

...cell, you will need to tag it with something that makes it easy to see under the microscope. The plasmids...Fluorescent Dyes and Ligands GFP Fusion Proteins — Making the Right Connection Photoactivatable Fluorescent...to FRET Bimolecular Fluorescence Complementation Makes Visualizing Protein–Protein Interactions Easy Split...

...expression systems. Addgene partnered with Ginkgo to make these plasmids available to scientists in academic...Codon-optimized expression (recode) Truncated for making pseudovirus (Ctrunc) ID Plasmid Description Mutations...

...plasmids and backbones with high cloning potential. By making these plasmids available as cgDNA, we hope to aid...note that this does not guarantee that Addgene will make cloning grade DNA preps of this plasmid available...

...protocols for how to use your viral prep and how to make your own preps. Recipient Instructions What to do...purified DNA from the original plasmid that was used to make the virus. In order for Addgene to provide viral...

...engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML, Thibodeau-Beganny...

...purified DNA from the original plasmid that was used to make the virus. For more details, see our FAQ on What... vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our ...

...plasmids. Animals - Antibodies produced in animals make use of the animal’s immune system. The antigen of...Overview of application Collect and lyse samples to make proteins available. Tissue samples may need additional...Ready to put your new knowledge to use? Addgene now makes ready-to-use recombinant antibodies made from plasmids...

... expression. Retroviral vectors are popular for making stable cell lines or clinical use. Adenoviral Vector...BioRender.com. Wild-type viruses have one goal, which is to make more viruses. The viral genome contains only the...

...finger nucleases (ZFNs) are effective tools for making targeted mutations in zebrafish. With support ...engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML et al....

...perform comprehensive quality control processes to make sure it is ready for your experiments. Shipping ...experience, from methods to troubleshooting insights, you make research more reproducible for scientists everywhere...

... 18 TAL effector repeats. The kit can be used to make arrays from 12-31 repeats. Perhaps one of the most...TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt...

...spike protein constructs for making VSV pseudo virus. Addgene blog Scientists Map the...opens in a new window) Kogiso, H., Raveau, M., Yamakawa, K., Saito, D., Ikeuchi, Y., Okazaki, T., Asano...

...of target sites that flox your genes of interest makes FLEx switch a powerful experimental tool. For more...transfection efficiency, selecting desired clones, and making transgenic mice. ID Plasmid Description Gene/Insert...

...Renilla luciferase have short protein half-lives, making them useful as transcriptional reporters, but are...a secreted version of the protein is available, making it ideal for a variety of applications. To learn...