We narrowed to 761 results for: Rest

1. General Transfection

   Type

   Protocol

   ...transfect mammalian cells with your plasmid of interest....Transfections allow for transient expression of a gene of interest in a target cell line and can be useful for short...

2. Working 9-5: an Addgene Co-op

   Type

   Blog Post

   ...description for this co-op, I was immediately interested in applying. This had stood out to me because...

3. Scientific Sharing in the Time of COVID-19: Databases and Resources

   Type

   Blog Post

   ...investigate COVID-19, and once universities reopen and restart other research, scientific sharing will remain ...

4. Supporting Rare Disease Research with BeHeard 2017

   Type

   Blog Post

   ...small molecules for use in the clinic and thereby restore motor neuron function in spinal muscular atrophy...

5. pSiM24: Simplifying Plant Genetic Engineering

   Type

   Blog Post

   ...also replaced the promoter regulating the gene of interest with a constitutive, robust M24 promoter. Originally...

6. Cre-ating New Methods for Site-specific Recombination in Drosophila

   Type

   Blog Post

   ... and you don't work in Drosophila, you may be interested to know that Rubin's team found that two of these...

7. Future of Research Conference - Remarkable Opening Session

   Type

   Blog Post

   ...action Each of the speakers in the panel had interesting words of experience and encouragement.  Dr. Sibby...

8. London Calling - Addgene’s New Europe Office in London

   Type

   Blog Post

   ...discoveries.   10 Years of Plasmid Sharing: Interested in more statistics from Addgene's 10 years of...

9. 9 Tips to Achieve Success in Academia

   Type

   Blog Post

   ... university? Ellis recommends that a student interested in leaving academia should present their research...

10. We're Updating Our Hold for Publication Policy

    Type

    Blog Post

    ... deposit due to experimental problems, legal restrictions, or any other reason. But this way, as soon ...

11. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type

    Blog Post

    ...suboptimal for HDR. At the AAVS1 locus, the two nearest gRNAs had cleavage sites 12 nt and 13 nt away from...

12. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type

    Blog Post

    ...collapse E. coli re-express arg1 gas vesicles and restore their ultrasound signal. Using acoustic reporter...

13. Antibodies 101: Fc Effector Functions

    Type

    Blog Post

    ... and resources References Clynes RA, Towers TL, Presta LG, Ravetch JV. Inhibitory Fc receptors modulate...

14. Cellosaurus: A Cell Line Information Database

    Type

    Blog Post

    ...find cells lines similar to your STR profiles of interest. You can filter CLASTR results by minimal number...

15. Hot Plasmids - October 2022

    Type

    Blog Post

    ... that can be incorporated into your protein of interest and recognized by reliable antibodies. It is derived...

16. Bringing Sustainable Practices to the Lab: Recycling

    Type

    Blog Post

    ...does cost money, but your institute may still be interested as it offsets the cost of regular waste services...

17. Year of the Camelid: Antibody Style

    Type

    Blog Post

    ...generate new sdAbs. There has also been some interesting work done developing transgenic mice that express...

18. Hot Plasmids and Viral Preps - July 2021

    Type

    Blog Post

    ...generate organoid cultures with a modification of interest. While the authors optimized the workflow for ...

19. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type

    Blog Post

    ...deactivation Prolonged activation Targeting Further restricted to illuminated region Impacts all cells expressing...

20. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type

    Blog Post

    ... tools used in modern molecular biology like restriction enzymes and polymerases. In the situation where...