Addgene

We narrowed to 651 results for: SIM;

Showing: 561 - 580 of 651 results

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Hot Plasmids and Viral Preps - March 2021

Type

Blog Post

...system from Jiazhang Lian’s lab that enables simultaneous gene activation, repression, and editing with...
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

Type

Blog Post

...activity, commonly via light-activated ion channels. Similarly, chemogenetic tools are designed channels that...
Engaging with science and society at pgEd

Type

Blog Post

...and how you can set yourself on a path towards a similar position. How pgEd broadens the conversation about...
Design Tips for Prime Editing

Type

Blog Post

...Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt...
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

Type

Blog Post

... of the transcribed ncRNA (Jiang et al. 2014). Simple knock-out experiments are trickier when it comes...
Antibodies 101: Stripping and Reprobing Western Blots

Type

Blog Post

...first round of detection are actually gone. First, simply repeat the imaging step to check if any secondary...
5 Tips for Troubleshooting Viral Transductions

Type

Blog Post

...your virus experiments. References 1. Anthony, Simon J., et al. "A strategy to estimate unknown viral...
Tips for Titering Your Lentiviral Preps

Type

Blog Post

... parallel, this approach should be considered. Similar to the p24 assay, titering via measurement of viral...
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

Type

Blog Post

...long hypothesized that life started with RNA and a simple cell membrane. His recent work shows that they ...
Hot Plasmids February 2024

Type

Blog Post

...mStayGold (Ando et al., 2023). This variant has similar photostability and brightness compared to the original...
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

Type

Blog Post

...after receiving her degree she was faced with a similar conundrum – she had the skills, training, and motivation...
10 Basic tips for mammalian cell culture

Type

Blog Post

...identified. Even if some mammalian cells lines have similar morphologies, being familiar with these morphologies...
xCas9: Engineering a CRISPR Variant with PAM Flexibility

Type

Blog Post

...xCas9 3.7. Base editor variants with xCas9 are similarly flexible. Like the xCas9 3.7 nuclease, xCas9 3.7...
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

Type

Blog Post

... that can bind effectively to protein targets, similar to how antibodies do. The structure of the common...
Cloning Mammalian Cells with the Agarose Method

Type

Blog Post

...procedure (illustrated in the YouTube video below) is simple: After obtaining antibiotic-resistant colonies,...
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

Type

Blog Post

...examine your images using subjective judgement by simply “looking at them”. Modern cameras and image analysis...
Fluorescent Proteins 101: Fluorescent Protein Timers

Type

Blog Post

...protein or protein fusion. This one protein method simplifies cloning and makes your experimental setup less...
The Scientific Conference Poster Session: Tips for Success

Type

Blog Post

...difficulty answering questions. Or perhaps you’re simply attending, but uncertain how to capitalize on your...
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

Type

Blog Post

... design to analyzing results. To use this tool, simply follow these steps: Create a new experiment in...
Of Myc and Men

Type

Blog Post

...for the foreseeable future. These newer findings simply add to our understanding of how this antibody works...

Showing: 561 - 580 of 651 results

Results per page:

Hot Plasmids and Viral Preps - March 2021

Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

Engaging with science and society at pgEd

Design Tips for Prime Editing

CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

Antibodies 101: Stripping and Reprobing Western Blots

5 Tips for Troubleshooting Viral Transductions

Tips for Titering Your Lentiviral Preps

Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

Hot Plasmids February 2024

Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

10 Basic tips for mammalian cell culture

xCas9: Engineering a CRISPR Variant with PAM Flexibility

No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

Cloning Mammalian Cells with the Agarose Method

Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

Fluorescent Proteins 101: Fluorescent Protein Timers

The Scientific Conference Poster Session: Tips for Success

CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

Of Myc and Men

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