We narrowed to 660 results for: SIM;

1. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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   Blog Post

   ...based delivery techniques may hold the key. In the simplest form, translating gene therapies from conceptual...

2. 5 Tips for Troubleshooting Viral Transductions

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   Blog Post

   ...your virus experiments. References 1. Anthony, Simon J., et al. "A strategy to estimate unknown viral...

3. Tips for Titering Your Lentiviral Preps

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   Blog Post

   ... parallel, this approach should be considered. Similar to the p24 assay, titering via measurement of viral...

4. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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   Blog Post

   ...long hypothesized that life started with RNA and a simple cell membrane. His recent work shows that they ...

5. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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   Blog Post

   ...after receiving her degree she was faced with a similar conundrum – she had the skills, training, and motivation...

6. 10 Basic tips for mammalian cell culture

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   Blog Post

   ...identified. Even if some mammalian cells lines have similar morphologies, being familiar with these morphologies...

7. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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   Blog Post

   ...xCas9 3.7. Base editor variants with xCas9 are similarly flexible. Like the xCas9 3.7 nuclease, xCas9 3.7...

8. Cloning Mammalian Cells with the Agarose Method

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   Blog Post

   ...procedure (illustrated in the YouTube video below) is simple: After obtaining antibiotic-resistant colonies,...

9. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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   Blog Post

   ...examine your images using subjective judgement by simply “looking at them”. Modern cameras and image analysis...

10. Fluorescent Proteins 101: Fluorescent Protein Timers

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    Blog Post

    ...protein or protein fusion. This one protein method simplifies cloning and makes your experimental setup less...

11. The Scientific Conference Poster Session: Tips for Success

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    Blog Post

    ...difficulty answering questions. Or perhaps you’re simply attending, but uncertain how to capitalize on your...

12. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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    Blog Post

    ... design to analyzing results. To use this tool, simply follow these steps: Create a new experiment in...

13. Production of Virus in Insect Versus Mammalian Cells

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    Blog Post

    ...Processing of your baculovirus is straightforward — simply spin down the supernatant in a centrifuge to remove...

14. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    Blog Post

    ... that can bind effectively to protein targets, similar to how antibodies do. The structure of the common...

15. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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    Blog Post

    ... of different pegRNAs for each desired edit, it simply becomes impossible (or at least unreasonable) to...

16. Pooled Library Amplifications

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    Blog Post

    ...looking at protocols used for libraries that are similar in size and composition as examples. Table 1 provides...

17. Twenty Years of Addgene Sharing: CRISPR

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    Blog Post

    ... and requesting each year (Figure 2). We did a similar analysis with CRISPR pooled libraries, although...

18. Plasmids 101: Degron Tags

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    Blog Post

    ...which target E3 ligases to specific proteins by simultaneously binding both their target protein and an E3...

19. Of Myc and Men

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    Blog Post

    ...for the foreseeable future. These newer findings simply add to our understanding of how this antibody works...

20. Google Forums Round Up: First Impressions of NgAgo

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    Blog Post

    ...inexpensive and can be 5’-phosphorylated in house using a simple PNK reaction or can be purchased 5’-modified from...