We narrowed to 730 results for: SON

...restriction enzyme, ligation independent, Gateway, Gibson, etc). The cloning method is ultimately chosen ...

...off-target effects.   New from the viral service By Jason Nasse We regularly add new viral aliquots from our...

...post was co-authored by Susanna Stroik and Rachel Leeson. Here at Addgene, we like to help share useful ...

...data about a disease or therapeutic Side-by-side comparison of the same experiment from different cell lines...

...practical understanding of how to apply it. The personal interaction of critiquing others’ manuscripts ...

...months down the line!) and to visit scientists in person to figure out ways we can make Addgene better and...

...found myself extremely grateful to my colleague Jason for choosing Simon Singh's "The Code Book" for this...

... Lianna Swanson...

...has multiple benchmarks. Think about what your personal goals are and set milestones along the way so ...

...467-477. Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR.  Nature. 2013 Jan; 493, 429–432. Cong L,...

...mismatches or indels. Empirical tests indicated that a reasonable rule is to require at least 96% sequence identity...

... Lianna Swanson...

...shift won’t happen overnight, but there are a few reasons why you might choose NgAgo over CRISPR proteins...

...to add overhangs to your insert of interest for Gibson assembly. The steps following primer design and...

...Plasmid Serotype Name 124603 AAV9 pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 124650 AAV9 pAAV-CamKIIa-C1V1...

...she will start her own research group at Boyce Tompson Institute.  References Baldwin, Melinda. "Credibility...

...Resources on Addgene.org Learn about the Michael Davidson fluorescent protein collection Check out Addgene...

...introduced at ~2 copies per cell per color, following a Poisson distribution. To overcome the limited expression...

...purification of tTALE-eGFP and tTALE-mCherry. In comparison to intercalating fluorescent dyes which can cause...

...strands that are packaged at the same frequency, we reasoned that, following DNA extraction, the [+] and [-...