We narrowed to 718 results for: STO

1. Addgene’s Viral Service - Why Virus? Why Now?

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   Blog Post

   ...people and organizations who helped us reach this milestone. Viral service supporters We would like to thank...

2. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   Blog Post

   ...differentiation. Furthermore, TALEN and ZFN require custom nucleases for each gene of interest. CRISPR-Cas9...

3. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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   Blog Post

   ...podcast segment As the primary cause for both the stomach flu and food-borne illnesses, noroviruses can infect...

4. Viral Vectors 101: The AAV Retrograde Serotype

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   Blog Post

   ...mechanisms by which retrograde AAV work are not yet understood, one thing is clear: this application opens a...

5. Viral Vectors 101: Viral Applications

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   Blog Post

   ...in your gene of interest, for example. Some customization is required, but many viral constructs have ...

6. Viral Vectors 101: Viruses as Biological Tools

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   Blog Post

   ...associations: common colds, COVID-19, norovirus (the stomach flu), and many more. Their infectious nature allows...

7. Plasmids 101: Methylation and Restriction Enzymes

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   Blog Post

   ...these strains should not be used for long term storage. Additional Resources Review of restriction modification...

8. Plasmids 101: Yeast Vectors

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   Blog Post

   ...promote replication.  Selection markers for yeast Historically, scientists have utilized auxotrophic selection...

9. Kiran Musunuru on the Newest TALEN Genome-Editing System

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   Blog Post

   ...modeling in stem cells. Addgene: Tell me about the history. How had investigators done this kind of work before...

10. CRISPR Guide

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    Guide

    ...insertions, or frameshift mutations leading to premature stop codons within the open reading frame (ORF) of the... cytosines in a gene’s promoter or by inducing histone acetylation or demethylation. The most common modifiers... green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence microscopy...gene by shifting the ORF and/or creating premature stop codons NHEJ N on- H omologous E nd J oining; A DNA...

11. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    Blog Post

    ...rhodopsins, are highly constrained and poorly understood. It’s hard to design new channelrhodopsins with...

12. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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    Blog Post

    ...at University of Texas Health Science Center at Houston. Prakash became interested in studying TS when ...

13. RNA Extraction Without A Kit

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    Blog Post

    ...sample(s) are resuspended in water or TE buffer, store them in a -80°C freezer to prevent RNA degradation...

14. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    Blog Post

    ...dimerize, bringing each terminus of Cre together to restore Cre activity. As soon as red light or PCB is no...

15. Antibodies 101: Selecting the Right Antibody

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    Blog Post

    ...be variations within an application. Take immunohistochemistry (IHC), for instance. While an antibody may...

16. Transferable Skills Guide: Problem Solving

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    Blog Post

    ...it is for helping your team reach an important milestone at work.   While I was in graduate school and ...

17. What's New in CRISPR - November 2021

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    Blog Post

    ... within the FAH gene was precisely corrected, restoring expression in the liver.  Read the paper in Nature...

18. Antibodies 101: Polyclonal Antibodies

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    Blog Post

    ...Fishman JB, Berg EA (2019) Antibody Purification and Storage. Cold Spring Harb Protoc 2019:pdb.top099101. https...

19. CRISPRainbow and Genome Visualization

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    Blog Post

    ...the 4D nucleome. Visualizing specific loci has historically been difficult, as techniques like fluorescent...

20. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    Blog Post

    ...ccdB accomplish this? Let’s start with a brief history of the gene and how molecular biologists have harnessed...