We narrowed to 712 results for: STO
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TypeBlog Post...these strains should not be used for long term storage. Additional Resources Review of restriction modification...
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Plasmids 101: Yeast Vectors
TypeBlog Post...promote replication. Selection markers for yeast Historically, scientists have utilized auxotrophic selection... -
Kiran Musunuru on the Newest TALEN Genome-Editing System
TypeBlog Post...modeling in stem cells. Addgene: Tell me about the history. How had investigators done this kind of work before... -
Gamma-Retroviral Vector Guide
TypeGuide...VSV-G due to wide infectivity VSV-G (vesicular stomatitis virus G protein) is the most common envelope ...supernatant containing the viral particles is removed and stored or centrifuged to concentrate. Crude or concentrated... The viral envelope gene; typically vesicular stomatitis virus G glycoprotein (VSV-G), an envelope protein... -
RNA Extraction Without A Kit
TypeBlog Post...sample(s) are resuspended in water or TE buffer, store them in a -80°C freezer to prevent RNA degradation... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog Post...dimerize, bringing each terminus of Cre together to restore Cre activity. As soon as red light or PCB is no... -
Transferable Skills Guide: Cross-team Communication
TypeBlog Post...service,” did he mean it the same way as Bryan, our customer support manager? When Gizela, the aforementioned... -
Viral Vectors 101: An Introduction to AAV
TypeBlog Post...-based method for the titration of AAV-2 vector stocks."Molecular Therapy 6.2 (2002): 272. PubMed PMID... -
All in a Twist: dsRNA
TypeBlog Post... some exist as dsRNA. Still others use dsDNA to store their genetic information. All of these viruses ... -
Antibodies 101: Selecting the Right Antibody
TypeBlog Post...be variations within an application. Take immunohistochemistry (IHC), for instance. While an antibody may... -
Transferable Skills Guide: Problem Solving
TypeBlog Post...it is for helping your team reach an important milestone at work. While I was in graduate school and ... -
What's New in CRISPR - November 2021
TypeBlog Post... within the FAH gene was precisely corrected, restoring expression in the liver. Read the paper in Nature... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog Post...rhodopsins, are highly constrained and poorly understood. It’s hard to design new channelrhodopsins with... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog Post...at University of Texas Health Science Center at Houston. Prakash became interested in studying TS when ... -
Antibodies 101: Polyclonal Antibodies
TypeBlog Post...Fishman JB, Berg EA (2019) Antibody Purification and Storage. Cold Spring Harb Protoc 2019:pdb.top099101. https... -
Visualizing Protein Turnover In Situ
TypeBlog Post...science instructor at The Innovation Institute in Boston. When Eugenia is not by the microscope she likes... -
CRISPRainbow and Genome Visualization
TypeBlog Post...the 4D nucleome. Visualizing specific loci has historically been difficult, as techniques like fluorescent... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog Post...ccdB accomplish this? Let’s start with a brief history of the gene and how molecular biologists have harnessed... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog Post...blogger Londa Schiebinger, PhD, Hinds Professor of History of Science, Stanford University. Sex and gender... -
CRISPR Guide
TypeGuide...insertions, or frameshift mutations leading to premature stop codons within the open reading frame (ORF) of the... cytosines in a gene’s promoter or by inducing histone acetylation or demethylation. The most common modifiers... green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence microscopy...gene by shifting the ORF and/or creating premature stop codons NHEJ N on- H omologous E nd J oining; A DNA...