We narrowed to 613 results for: Set

1. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

   Type

   Blog Post

   ...random and directed mutagenesis approach, the Anna Cereseto Lab created pX-evoCas9, which they showed also...

2. A Deep Dive into BioBuilder

   Type

   Blog Post

   ...High School Apprenticeship, preparing local (Massachusetts), economically disadvantaged students for paid...

3. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

   Type

   Blog Post

   ...Transformation of Drosophila via PhiC31 Integrase-Mediated Cassette Exchange. Genetics. 173, 769–777 (2006). PubMed...

4. Screening for Successful Genome Editing with Digital PCR

   Type

   Blog Post

   ...Partitions that did not contain any target DNA at the onset of PCR are negative, whereas those that did are ...

5. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

   Type

   Blog Post

   ... vectors for use with their favorite selection cassette. They could also switch from FLAG to GFP or a ...

6. Plasmids 101: Yeast Vectors

   Type

   Blog Post

   ...markers. The MX series of antibiotic resistance cassettes is most commonly employed, with the KanMX being...

7. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

   Type

   Blog Post

   ...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...

8. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

   Type

   Blog Post

   ...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...

9. The Future of Research Symposium Boston 2015

   Type

   Blog Post

   ...University School of Medicine and the University of Massachusetts Medical School. Graduate education programs ...

10. Plasmids 101: Cre-lox

    Type

    Blog Post

    ...either side (often called a “lox-stop-lox” or “LSL” cassette) upstream of a gene of interest will prevent gene...

11. Viral Vectors 101: Calcium Sensors

    Type

    Blog Post

    ...and is a great FRET sensor option if you have the setup necessary for readout.   We’ve got a live...

12. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type

    Blog Post

    ...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...

13. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type

    Blog Post

    ...the home cage and places it into the experimental setup, the mouse becomes stressed. It will likely move...

14. Fluorescent Proteins 101: Introduction to FRET

    Type

    Blog Post

    ...complex. To help troubleshoot your experimental setup as a potential source of error in suboptimal or ...

15. Plan Your Experiment

    Type

    Guide

    ...to NHEJ knockout. Base editors can make a limited set of mutations. Repress or Interfere (CRISPRi) Reduce...

16. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type

    Blog Post

    ...and a plasmid that contains the Mobile CRISPRi cassette flanked by left and right Tn7 end sequences. Donor...

17. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type

    Blog Post

    ...after completing her postdoctoral training from Massachusetts General Hospital and Harvard Medical School....

18. Plasmids 101: Protein Expression

    Type

    Blog Post

    ...amounts of protein in bacterial cells: the pET, pRSET, Gateway pDEST, and pBAD vectors for example. Protein...

19. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type

    Blog Post

    ...enough to carry the Cas9 gene and a gRNA expression cassette in one single viral particle. The earliest explorations...

20. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type

    Blog Post

    ...As we pointed out last year2, delivering a DNA cassette encoding the cas9 gene and single guide RNAs (...