We narrowed to 618 results for: Set

1. Addgene Depositors Get More Citations

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   ...numbers. Note: this does drop some journals from our dataset, since not all journals have papers with plasmids...

2. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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   ...random and directed mutagenesis approach, the Anna Cereseto Lab created pX-evoCas9, which they showed also...

3. Viral Vectors 101: Inverted Terminal Repeats

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   ...Recombinant AAV vector with ITRs that flank the gene cassette, which contains a promoter, the gene of interest...

4. Viral Vectors 101: Viral Vector Elements

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   ...vectors Of the many viruses out there, only a small subset are commonly used as tools in the lab. We will ...

5. Viral Vectors 101: Viral Applications

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   ...be used to express proteins. Viral expression cassettes typically contain a promoter (inducible or constitutive...

6. Sharing is Caring: But How to Distribute Open Hardware?

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   ... tailored image processing and low-cost optical setups. References Joel T. Collins, Joe Knapper, Julian...

7. A Deep Dive into BioBuilder

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   ...High School Apprenticeship, preparing local (Massachusetts), economically disadvantaged students for paid...

8. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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   ...Transformation of Drosophila via PhiC31 Integrase-Mediated Cassette Exchange. Genetics. 173, 769–777 (2006). PubMed...

9. Screening for Successful Genome Editing with Digital PCR

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   ...Partitions that did not contain any target DNA at the onset of PCR are negative, whereas those that did are ...

10. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    ... vectors for use with their favorite selection cassette. They could also switch from FLAG to GFP or a ...

11. Plasmids 101: Yeast Vectors

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    ...markers. The MX series of antibiotic resistance cassettes is most commonly employed, with the KanMX being...

12. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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    ...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...

13. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    ...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...

14. The Future of Research Symposium Boston 2015

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    ...University School of Medicine and the University of Massachusetts Medical School. Graduate education programs ...

15. Plasmids 101: Cre-lox

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    ...either side (often called a “lox-stop-lox” or “LSL” cassette) upstream of a gene of interest will prevent gene...

16. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    ...the home cage and places it into the experimental setup, the mouse becomes stressed. It will likely move...

17. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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    ...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...

18. Viral Vectors 101: Calcium Sensors

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    ...and is a great FRET sensor option if you have the setup necessary for readout.   We’ve got a live...

19. Fluorescent Proteins 101: Introduction to FRET

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    ...complex. To help troubleshoot your experimental setup as a potential source of error in suboptimal or ...

20. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    ...and a plasmid that contains the Mobile CRISPRi cassette flanked by left and right Tn7 end sequences. Donor...