...neuroscience research. More broadly, advances in gene synthesis, affinity reagent screening, and next-generation...

...designed a calcineurin transcriptional reporter consisting of a quickly-degraded destabilized GFP variant...

...Central PMCID: PMC427010. Porter, R. (1959). The hydrolysis of rabbit y-globulin and antibodies with crystalline...

...AHC suffer from recurrent attacks of unilateral paralysis (hemiplegia) that are often painful and accompanied...

...selection cassette containing genes for hygromycin resistance and the Roller movement phenotype. To make these...

...control The CRISPR interference (CRISPRi) model consists of a catalytically dead Cas9 protein (dCas9) that...

...became aggressive. If we can understand the neural basis of violence or aggression, it might do a lot to ...

...lentiviral vectors deliver mammalian antibiotic resistance (e.g., puromycin, blasticidin), which enables...not all lentiviral vectors deliver antibiotic resistance. This protocol was established using 293T cells...transgene expression. As polyclonal populations of resistant cells start proliferating and the individual wells...

...molecular cloning consist of: Leader Sequence: Extra base pairs on the 5' end of the primer assist with restriction...selecting restriction sites, you should use a DNA analysis tool, such as Addgene’s Sequence Analyzer , to...

...3D models of the human lymphatic system. PBMCs consist of various immune cells, and can be used as a whole...

...the strain tractable for peroxisomal studies on biogenesis, degradation, and proliferation. Genome manipulation...

...HiFi can simplify multi-position site-directed mutagenesis by assembling multiple fragments containing the...

...References 1. Ingolia, Nicholas T., et al. “Genome-wide analysis in vivo of translation with nucleotide resolution...

...sample. The second sensor is a fusion protein consisting of sfGFP fused to a His-tagged ABC transporter...

...technologies that permit large-scale creation and synthesis (“writing”) of longer pieces of synthetic DNA,...

...the break and 91bp on the PAM-proximal side, consistently supported higher frequencies (up to 60%!) of...

...historical notes CRISPR arrays in bacterial genomes consist of repeated elements separated by unique sequences...

...lineages, 2-photon spectra, more elaborate spectrum analysis tools, and a better way to store and represent...

...plants, among others. I particularly enjoyed the emphasis that microbes are neither bad or good - they're...

...industry”. To date, aside from doing science, he has assisted many researchers in gaining the confidence to ...