We narrowed to 731 results for: Sis;

1. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

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   Blog Post

   ...fluorophore, or APEX2), allowing for quantitative analysis using different detection methods. Since the anti-GFP...

2. Viral Vectors 101: Transductions

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   Blog Post

   ...non-integrating. Read on to find out more! Molecular basis of transduction Transductions utilize the ‘normal...

3. Hot Plasmids and Viral Preps - September 2021

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   Blog Post

   ...selection marker, Thy1.1, in addition to puromycin resistance.  The MYC-CRISPR library targets E-boxes genome-wide...

4. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

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   Blog Post

   ...The lab saw that the B.1.351 variant was very resistant to vaccine serum responses. This result was similar...

5. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

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   Blog Post

   ...neuroscience research. More broadly, advances in gene synthesis, affinity reagent screening, and next-generation...

6. What the HEK?

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   Blog Post

   ...many immature neuron-like qualities, which is inconsistent with kidney cells (Shaw et al.). Since then,...

7. Donations from Addgene to Yield Answers for Rare Disease Researchers

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   Blog Post

   ...AHC suffer from recurrent attacks of unilateral paralysis (hemiplegia) that are often painful and accompanied...

8. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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   Blog Post

   ...selection cassette containing genes for hygromycin resistance and the Roller movement phenotype. To make these...

9. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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   Blog Post

   ...control The CRISPR interference (CRISPRi) model consists of a catalytically dead Cas9 protein (dCas9) that...

10. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

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    Blog Post

    ...became aggressive. If we can understand the neural basis of violence or aggression, it might do a lot to ...

11. Kit Free RNA Extraction

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    Protocol

    ...general steps of this protocol include homogenization/lysis of cells or tissues, extraction of RNA, precipitation...isolation will depend on how effective your cell lysis protocol is. While simple homogenization is effective...

12. Celebrate National Book Month with Addgene

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    Blog Post

    ...plants, among others. I particularly enjoyed the emphasis that microbes are neither bad or good - they're...

13. The Crispr Babies Saga Shows the Need for Action, Not More Delays

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    Blog Post

    ...report is that when it became clear that this assistive reproductive technique was feasible, three separate...

14. 25 Work From Home Tips for Scientists

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    Blog Post

    ...communities are looking for bench scientists to assist in labs that are testing for COVID-19. You can ...

15. FPbase: A new community-editable fluorescent protein database

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    Blog Post

    ...lineages, 2-photon spectra, more elaborate spectrum analysis tools, and a better way to store and represent...

16. Revamp Your Lab Meetings With Creative Virtual Collaboration

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    Blog Post

    ...industry”. To date, aside from doing science, he has assisted many researchers in gaining the confidence to ...

17. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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    Blog Post

    ...., Vitriol, E., & Hahn, K. (2008). Chromophore-assisted laser inactivation in cell biology. Trends in ...

18. Pushing the Limits of DNA Assembly

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    Blog Post

    ...HiFi can simplify multi-position site-directed mutagenesis by assembling multiple fragments containing the...

19. Visualizing Translation at the Single Molecule Level

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    Blog Post

    ...References 1. Ingolia, Nicholas T., et al. “Genome-wide analysis in vivo of translation with nucleotide resolution...

20. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

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    Blog Post

    ...sample. The second sensor is a fusion protein consisting of sfGFP fused to a His-tagged ABC transporter...