We narrowed to 738 results for: Sis;

1. Management for Scientists: Giving Feedback

   Type

   Blog Post

   ...Feedback is help and it should result in authentic assistance for the recipient to become more effective and...

2. Choosing Your Perfect Empty Backbone

   Type

   Blog Post

   ...with the proper selection marker or antibiotic resistance. Next, consider the expression level of your ...

3. Advice for Choosing a Research Project

   Type

   Blog Post

   ...to use to reach these milestones? Can you get assistance from people who have the required expertise to...

4. The Stingy Scientist: How the Baby Gel Box Was Born

   Type

   Blog Post

   ...before the advent of techniques like PCR and DNA synthesis. Most molecular biologists bought the pricey stuff...

5. Plasmid Cloning by PCR (with Protocols)

   Type

   Protocol

   ...molecular cloning consist of: Leader Sequence: Extra base pairs on the 5' end of the primer assist with restriction...selecting restriction sites, you should use a DNA analysis tool, such as Addgene’s Sequence Analyzer , to...

6. Viral Vectors 101: Transductions

   Type

   Blog Post

   ...non-integrating. Read on to find out more! Molecular basis of transduction Transductions utilize the ‘normal...

7. What the HEK?

   Type

   Blog Post

   ...many immature neuron-like qualities, which is inconsistent with kidney cells (Shaw et al.). Since then,...

8. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

   Type

   Blog Post

   ...fluorophore, or APEX2), allowing for quantitative analysis using different detection methods. Since the anti-GFP...

9. Hot Plasmids and Viral Preps - September 2021

   Type

   Blog Post

   ...selection marker, Thy1.1, in addition to puromycin resistance.  The MYC-CRISPR library targets E-boxes genome-wide...

10. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type

    Blog Post

    ...The lab saw that the B.1.351 variant was very resistant to vaccine serum responses. This result was similar...

11. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type

    Blog Post

    ...neuroscience research. More broadly, advances in gene synthesis, affinity reagent screening, and next-generation...

12. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type

    Blog Post

    ...designed a calcineurin transcriptional reporter consisting of a quickly-degraded destabilized GFP variant...

13. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type

    Blog Post

    ...Central PMCID: PMC427010. Porter, R. (1959). The hydrolysis of rabbit y-globulin and antibodies with crystalline...

14. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type

    Blog Post

    ...AHC suffer from recurrent attacks of unilateral paralysis (hemiplegia) that are often painful and accompanied...

15. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type

    Blog Post

    ...selection cassette containing genes for hygromycin resistance and the Roller movement phenotype. To make these...

16. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type

    Blog Post

    ...control The CRISPR interference (CRISPRi) model consists of a catalytically dead Cas9 protein (dCas9) that...

17. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type

    Blog Post

    ...became aggressive. If we can understand the neural basis of violence or aggression, it might do a lot to ...

18. Kit Free RNA Extraction

    Type

    Protocol

    ...general steps of this protocol include homogenization/lysis of cells or tissues, extraction of RNA, precipitation...isolation will depend on how effective your cell lysis protocol is. While simple homogenization is effective...

19. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type

    Blog Post

    ...3D models of the human lymphatic system. PBMCs consist of various immune cells, and can be used as a whole...

20. K. phaffii: Rising to the Occasion

    Type

    Blog Post

    ...the strain tractable for peroxisomal studies on biogenesis, degradation, and proliferation. Genome manipulation...