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  1. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...interest allowing for insertion in the correct orientation. These plasmids use a novel synthetic RPBSA promoter... exciting tool for producing specific genetic mutations and gene disruptions without the use of viral ...

  2. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ...compare with your own perception? Tell me your interpretation of my feedback to you. What are We Learning...Steven Pinker. As a manager you must be able to state your observations truthfully and in clear terms....

  3. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...the introduction of a mutation into the genome, how can cells with that mutation be selected/screened?...

  4. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ... This tag has been used successfully for immunoprecipitation, western blot, and immunofluorescence with...has the flexibility of also being used for immunoprecipitation, purification, and western blot as monoclonal...

  7. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...extract.    Graphical Abstract: Schematic representation of cell-free system, capable of producing fluorescent...modify up to 60% of the disease-causing point mutations. They allow single-nucleotide modifications to...

  9. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...the protein folded, changing it from its native state to a less folded, or denatured, one. This enables... protein of interest while it’s in a transition state or specific configuration. So how do you ensure ...

  11. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...cleaving and rejoining supercoiled DNA ends to facilitate replication. The TOPO technique specifically ...Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis to Easily Generate Insertion and Deletions in...

  14. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...under CC BY from Mattei et.al., 2022.   Limitations of the in silico approach The in silico approach... verification. All approaches have their own limitations. Using methods together can inform decisions ...

  15. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...makes it easier to spot even minor weakness or limitations in a piece of research (Drummond & Fischhoff,... the National Academy of Sciences of the United States of America, 114(36), 9587-9592. doi:10.1073/pnas...

  16. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...translated in a specific compartment. Despite these limitations, these techniques represent powerful tools for...translation in various cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide...

  18. The Power Behind NGS Plasmid Validation: seqWell

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    Blog Post
    ...below. Normalization is a fundamental and often understated problem in multiplex NGS library preparation....seqWell, Inc. He is a sequencing technologist and computational biologist with a passion for solving critical...

  19. How to Lead a Great Meeting

    Type
    Blog Post
    ... the purpose of the meeting: Begin meetings by stating “The purpose of this meeting is to _________." ...ambassador” from each affected team to be the representative in the meeting and then communicate outcomes...

  20. Components of CRISPR/Cas9

    Type
    Blog Post
    ...LbCpf1 RR variant 5' TYCV AsCpf1 RVR variant 5' TATV Campylobacter jejuni (CJ) 3' NNNNRYAC  Neisseria... as a scaffold linking the crRNA to Cas9 and facilitates processing of mature crRNAs from pre-crRNAs derived...
Showing: 561 - 580 of 836 results