We narrowed to 797 results for: Tec
-
TypeBlog Post...the Associate Director and Head of Gene Editing Technology at Beam Therapeutics, used a previously developed...activity and therapeutic application. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0491-6 Additional...
-
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post...Partnering with seqWell, we are using the plexWell technology to easily and quickly create Illumina sequencing...tags, fusion proteins, and selectable markers by detecting common features using Snapgene. If we find information... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog Post...10.1021/acssynbio.9b00395 O’Brian, SP, et al., Biotechnol J. 2014. https://pubmed.ncbi.nlm.nih.gov/24390935...as time-lapse imaging or with high light power detection of molecules. Lee, J. et al., Science Advances... -
6 Tips for Grant Writing
TypeBlog Post...network of your peers. Furthermore, using new technologies to solve a problem can allow you to approach...different labs during your career; you learn new techniques and interact with new people. If you are looking... -
Transferable Skills Guide: Managing a Team
TypeBlog Post...to avoid mistakes due to miscommunication and to detect trouble. I’m not comfortable with awkward silences...someone more experienced to guide you - is valuable. Protect your team Make you team feel safe. Your team members... -
Degrading DNA with Cascade-Cas3
TypeBlog Post...Yoshimi, K., & Mashimo, T. (2022). Genome editing technology and applications with the type I CRISPR system...for genome engineering in human cells. Nature Biotechnology, 37(12), 1471–1477. https://doi.org/10.1038/... -
Magnetic Control of Proteins: More than a Dream
TypeBlog Post...Lots of scientists work to invent novel techniques or to engineer improved tools for familiar applications...expressing good ol’ EGFP — showed a slight but detectable change in fluorescence when a small magnet was... -
3D Printing Meets CRISPR Cas9
TypeBlog Post...physical models of proteins using 3D printing technology. The tagline for our company is “…where molecules...effective this model was in explaining CRISPR technology to 4th graders, check out Jennifer Doudna’s new... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog Post...amounts of protein for any such disease. This technology therefore represents a potential platform that...CRISPRs plasmid tools, find more plasmids, CRISPR technology guides, FAQs, and CRISPR resources on Addgene's... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog Post...your plasmid prep We'll discuss the following techniques to increase plasmid yield. Keep in mind that ...that you might need to use multiple techniques for a particularly stubborn prep. Check the bacterial strain... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post...expressing GFP from a given promoter, and the technique can be adapted to other transgenes. It’s important...dominance over HDR in this species. As CRISPR technology continues to develop, it’s become clear that ... -
Genome-wide Screening Using CRISPR
TypeBlog Post...advantage of CRISPR/Cas9 over previous genome editing techniques is its simplicity and versatility. CRISPR/Cas9...you'd like to brush up on the various CRISPR technologies, check out Addgene's CRISPR Guide. Figure... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog Post...the entirety of a cell. That's a big long-term technical challenge. We have a strategy that organizes how... into actionable signals. Let's say I wanted to detect every primary metabolite in a cell. The only way... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog Post...best teachers and mentors pass on more than just technical knowledge, shaping their trainees’ very way of.... The MCC develops and makes freely available technical and pedagogical resources, offers mentoring for... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog Post...knock-ins in mammalian cells. J Biotechnol., 16(4): 387-390 (2020). 10.1016/j.jbiotec.2019.11.010 Wright, D. W... -
CRISPR 101: RNA Editing with Cas13
TypeBlog Post...CRISPR-C2c2 enable guide-RNA processing and RNA detection." Nature 538(7624) (2016):270-273. PubMed PMID...Gootenberg, Jonathan S., et al. "Nucleic acid detection with CRISPR-Cas13a/C2c2." Science (2017): eaam9321... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog Post...them that their findings may be the result of a technical artifact that introduced an ancestry bias into... a reduction in the number of the dependencies detected. Given this new information, Misek notes that ... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog Post...CRISPR-Cas9–mediated gene inactivation." Nature biotechnology 32.12 (2014): 1262. PubMed PMID: 25184501. PubMed... off-target effects of CRISPR-Cas9." Nature biotechnology 34.2 (2016): 184. PubMed PMID: 26780180. PubMed... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog Post...Sanger sequencing-based analysis tools are unable to detect or analyze complex edits, such as those generated...sample, ICE includes visual representations of all detected edit types in the sample. The visualization helps... -
AAV Titers: Where Do They Come From And What Do They Mean?
TypeBlog Post...microscopy of a viral vector solution. Since this technique is intensive and requires an electron microscope...with titrating by qPCR are addressed with ddPCR technology (Hindson et al., 2011, Hindson et al., 2013),...